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Fresenius' Journal of Analytical Chemistry

, Volume 365, Issue 8, pp 682–684 | Cite as

Determination of catecholamines as their N-hydroxy-succinimidyl-3-indolylacetate derivatives by pre-column derivatization HPLC separation and fluorescent detection

  • Hong Wang
  • Hong Jin
  • Hua-shan Zhang
Short communication

Abstract

N-Hydroxysuccinimidyl-3-indolylacetate (SIIA) is a new fluorescent derivatizing reagent with an indole ring and ¶an N-hydroxysuccinimide ester functionality. It can react with catecholamines under mild conditions to form corresponding amides, which have strong fluorescence at λexem = 301 nm/¶365 nm. This paper covers the RP-HPLC separation and fluorescent determination of derivatized catecholamines with SIIA. In a mobile phase of methanol-water (36/64, v/v) containing H3cit-Na2HPO4 buffer (pH = 4.00, 10 mmol/L), the derivatives of norepinephrine (NE), epinephrine (E) and dopamine (DA) were eluted within 15 min on a C18 column. The detection limits were 0.043, 0.13 and 0.18 pmol, respectively, when the ratio of signal to noise (S/N) was 3. The excessive reagent is rapidly hydrolyzed to 3-indolylacetic acid (IA) that can be easily separated from derivatives.

Keywords

Dopamine Epinephrine Catecholamine Indole Strong Fluorescence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Hong Wang
    • 1
  • Hong Jin
    • 1
  • Hua-shan Zhang
    • 1
  1. 1.Department of Chemistry,Wuhan University, Wuhan 430072, ChinaCN

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