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Fresenius' Journal of Analytical Chemistry

, Volume 365, Issue 8, pp 658–662 | Cite as

Oriented antibody immobilization for atrazine determination by a flow-through fluoroimmunosensor

  • E. Turiel
  • P. Fernández
  • C. Pérez-Conde
  • A. M. Gutiérrez
  • C. Cámara
Original paper

Abstract

An atrazine flow-through fluoroimmunosensor was developed, based on an oriented antibody covalently bound to Protein-A (Prot-A) immobilized on Controlled Pore Glass (CPG). Atrazine was detected “in-situ” by placing the immobilized antibody in the optical path of the flow cell. Immobilization of 30 μg of polyclonal anti-atrazine antibody on 0.5 g of Prot-A-CPG provided the highest sensitivity. The effect of several solvents on the covalently immobilized antibodies regeneration was evaluated, the optimum conditions being achieved by pumping 5% acetonitrile (pH = 3) at 0.15 mL/min for 100 s. The detection limit of the immunosensor was 0.7 μg/L and the reproducibility was 2% and 4% for 5 μg/L and 40 μg/L, respectively, in the optimum working concentration range (0.7–50 μg/L). This device allowed 12 samples per hour to be analyzed and had a life-time of 200 assays. Simazine and desisopropylatrazine (DIA) were not cross-reactive, desethylatrazine (DEA) has a cross-reactivity of 8% and propazine and prometryn of 44% and 27%, respectively. The immunosensor was applied to the determination of atrazine in tap and ground water samples spiked at the ¶10 and 30 μg/L concentration level.

Keywords

Acetonitrile Immobilization Ground Water Atrazine Optical Path 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • E. Turiel
    • 1
  • P. Fernández
    • 1
  • C. Pérez-Conde
    • 1
  • A. M. Gutiérrez
    • 1
  • C. Cámara
    • 1
  1. 1.Departamento de Química Analítica, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, E-28040 Madrid, SpainES

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