Fresenius' Journal of Analytical Chemistry

, Volume 365, Issue 8, pp 654–657 | Cite as

Kinetic spectrophotometric determination of sulfide, using in-cuvette mixing and titration techniques with computerized data acquisition

  • B. Haghighi
  • A. Safavi
Original paper


In-cuvette mixing and titration techniques have been used for the kinetic determination of sulfide based on its inhibition effect on the oxidation of indigo carmine with hexacyanoferrate(III) in the presence of silver. The reaction was monitored spectrophotometrically by measuring the decrease in absorbance of indigo carmine at 612 nm. Both initial rate and fixed-time methods were applied to the in-cuvette technique. Using in-cuvette mixing, sulfide up to 1800 ng was determined and detection limit and relative standard deviation for the determination of 120 ng of sulfide were calculated as 23.0 ng and 1.59%, respectively. On the basis of the titration technique, the upper limit of determination was 25 μg of sulfide and detection limit and average relative standard deviation for the determination of 1 μg of sulfide were 0.025 μg and 4.95%, respectively. The effect of foreign ions on the sulfide determination was studied. The proposed methods were applied to the determination of sulfide in water.


Sulfide Titration Detection Limit Data Acquisition Relative Standard Deviation 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • B. Haghighi
    • 1
  • A. Safavi
    • 2
  1. 1.Department of Chemistry, Institute for Advanced Studies in Basic Sciences, Zanjan, Iran, 45195-159 e-mail:
  2. 2.Department of Chemistry, College of Sciences, Shiraz University, Shiraz, Iran, 71454 e-mail:

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