Congener-specific method for the determination of ortho and non-ortho polychlorinated biphenyls, polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans in foods by carbon-column fractionation and gas chromatography-isotope dilution mass spectrometry

Abstract

 The chromatographic behavior of ortho and non-ortho polychlorinated biphenyls (PCBs) on supported carbon columns has been investigated and the structure-affinity relationship of activated carbon towards PCB molecules established. Optimisation of the parameters controlling the elution of PCB congeners through the carbon column led to the development of a solvent scheme for the efficient separation of (i) ortho substituted PCBs, (ii) non-ortho substituted PCBs and (iii) polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) in three separate fractions. A method for the extraction, clean-up, fractionation and determination of ortho and non-ortho substituted PCBs by GC-isotope dilution MS was developed and validated by analysis of a certified reference material. Possible losses of PCBs during freeze-drying and interferences of aliphatic hydrocarbons during mass spectrometric determination of ortho substituted PCBs are also discussed.

Author information

Affiliations

Authors

Additional information

Received: 23 June 1995/Revised: 9 May 1996/Accepted: 25 May 1996

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Krokos, F., Creaser, C., Wright, C. et al. Congener-specific method for the determination of ortho and non-ortho polychlorinated biphenyls, polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans in foods by carbon-column fractionation and gas chromatography-isotope dilution mass spectrometry. Fresenius J Anal Chem 357, 732–742 (1997). https://doi.org/10.1007/s002160050240

Download citation

Keywords

  • Fractionation
  • Activate Carbon
  • PCBs
  • Biphenyl
  • Certified Reference Material