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Analytical strategy for studying the formation and stability of multilayered films containing gold nanoparticles

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Abstract

The design of layer-by-layer (LbL) polyelectrolyte films including nanoparticles is a growing field of innovation in a wide range of biomedical applications. Gold nanoparticles (AuNPs) are very attractive for further biomolecule coupling to induce a pharmacological effect. Nanostructured LbL films coupled with such metallic species show properties that depend on the conditions of construction, i.e. the polymer nature and dissolution buffer. Tripartite LbL films (polycation, AuNP, and polyanion) were evaluated using two different polycationic polymers (poly(allylamine hydrochloride) (PAH), poly(ethylene imine) (PEI)) and various medium conditions (salts, i.e. phosphate, Tris or Tris-NaCl buffers, and concentration). AuNP incorporation and film stability were analysed by visible spectrophotometry, capillary zone electrophoresis, a quartz crystal microbalance, and high-performance liquid chromatography. The ideal compromise between AuNP loading and film stability was obtained using PAH prepared in Tris-NaCl buffer (0.01–0.15 M). This condition allowed the formation of a LbL film that was more stable than the film with PEI and provided an AuNP quantity that was 4.8 times greater than that of the PAH-PBS-built film. In conclusion, this work presents an analytical strategy for the characterization of nanostructured multilayer films and optimization of LbL films enriched with AuNPs to design biomedical device coatings.

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References

  1. Acharya G, Lee CH, Lee Y. Optimization of cardiovascular stent against restenosis: factorial design-based statistical analysis of polymer coating conditions. Moretti C, editor. PLoS One. 2012;7(8):e43100.

    Article  CAS  Google Scholar 

  2. Kim B-S, Smith RC, Poon Z, Hammond PT. MAD (multiagent delivery) nanolayer: delivering multiple therapeutics from hierarchically assembled surface coatings †. Langmuir. 2009 Dec 15;25(24):14086–92.

    Article  CAS  Google Scholar 

  3. Pavlukhina S, Sukhishvili S. Polymer assemblies for controlled delivery of bioactive molecules from surfaces. Adv Drug Deliv Rev. 2011;63(9):822–36.

    Article  CAS  Google Scholar 

  4. Garza JM, Schaaf P, Muller S, Ball V, Stoltz J-F, Voegel J-C, et al. Multicompartment films made of alternate polyelectrolyte multilayers of exponential and linear growth. Langmuir. 2004;20(17):7298–302.

    Article  CAS  Google Scholar 

  5. Pallotta A, Clarot I, Sobocinski J, Fattal E, Boudier A. Nanotechnologies for medical devices: potentialities and risks. ACS Appl Bio Mater. 2019;2(1):1–13.

    Article  CAS  Google Scholar 

  6. Diamanti E, Andreozzi P, Anguiano R, Yate L, Gregurec D, Politakos N, et al. The effect of top-layer chemistry on the formation of supported lipid bilayers on polyelectrolyte multilayers: primary versus quaternary amines. Phys Chem Chem Phys. 2016;18(47):32396–405.

    Article  CAS  Google Scholar 

  7. Diamanti E, Andreozzi P, Kirby C, Anguiano R, Yate L, Heinz H, et al. Study of the impact of polyanions on the formation of lipid bilayers on top of polyelectrolyte multilayers with poly(allylamine hydrochloride) as the top layer. J Phys Chem B. 2017;121(5):1158–67.

    Article  CAS  Google Scholar 

  8. Wang Y, Guo X, Pan R, Han D, Chen T, Geng Z, et al. Electrodeposition of chitosan/gelatin/nanosilver: a new method for constructing biopolymer/nanoparticle composite films with conductivity and antibacterial activity. Mater Sci Eng C. 2015;53:222–8.

    Article  CAS  Google Scholar 

  9. Liu S, Liu S, Liu X, Zhao J, Cui W, Fan C. Antibacterial antiadhesion membranes from silver-nanoparticle-doped electrospun poly( L -lactide) nanofibers. J Appl Polym Sci. 2013;129(6):3459–65.

    Article  CAS  Google Scholar 

  10. Liu Y, Chen W, Kim H-I. Antibacterial activity of pH-sensitive genipin cross-linked chitosan/poly(ethylene glycol)/silver nanocomposites. Polym Adv Technol. 2012;23(1):8–14.

    Article  CAS  Google Scholar 

  11. Kim C-Y, Xu L, Lee E-H, Choa Y-H. Magnetic silicone composites with uniform nanoparticle dispersion as a biomedical stent coating for hyperthermia. Adv Polym Technol. 2013;32(S1):E714–23.

    Article  CAS  Google Scholar 

  12. Li W-T, Wang M-H, Li Y-J, Sun Y, Li J-C. Linker-free layer-by-layer self-assembly of gold nanoparticle multilayer films for direct electron transfer of horseradish peroxidase and H2O2 detection. Electrochim Acta. 2011;56(20):6919–24.

    Article  CAS  Google Scholar 

  13. Tian S, Liu J, Zhu T, Knoll W. Polyaniline/gold nanoparticle multilayer films: assembly, properties, and biological applications. Chem Mater. 2004;16(21):4103–8.

    Article  CAS  Google Scholar 

  14. Pallotta A, Parent M, Clarot I, Luo M, Borr V, Dan P, et al. Blood compatibility of multilayered polyelectrolyte films containing immobilized gold nanoparticles. Part Part Syst Charact. 2017;34(1):1600184.

    Article  Google Scholar 

  15. Mertz D, Vogt C, Hemmerlé J, Debry C, Voegel J-C, Schaaf P, et al. Tailored design of mechanically sensitive biocatalytic assemblies based on polyelectrolyte multilayers. J Mater Chem. 2011;21(23):8324.

    Article  CAS  Google Scholar 

  16. Yoshida K, Sato K, Ono T, Dairaku T, Kashiwagi Y. Preparation of Nafion/polycation layer-by-layer films for adsorption and release of insulin. Polymers. 2018;10(8):812.

    Article  Google Scholar 

  17. Decher G. Fuzzy nanoassemblies: toward layered polymeric multicomposites. Science. 1997;277(5330):1232–7.

    Article  CAS  Google Scholar 

  18. Pallotta A, Philippe V, Boudier A, Leroy P, Clarot I. Highly sensitive and simple liquid chromatography assay with ion-pairing extraction and visible detection for quantification of gold from nanoparticles. Talanta. 2018;179:307–11.

    Article  CAS  Google Scholar 

  19. Tournebize J, Boudier A, Sapin-Minet A, Maincent P, Leroy P, Schneider R. Role of gold nanoparticles capping density on stability and surface reactivity to design drug delivery platforms. ACS Appl Mater Interfaces. 2012;4(11):5790–9.

    Article  CAS  Google Scholar 

  20. Pallotta A, Boudier A, Leroy P, Clarot I. Characterization and stability of gold nanoparticles depending on their surface chemistry: contribution of capillary zone electrophoresis to a quality control. J Chromatogr A. 2016;1461:179–84.

    Article  CAS  Google Scholar 

  21. Beurton J, Clarot I, Stein J, Creusot B, Marcic C, Marchioni E, et al. Long-lasting and controlled antioxidant property of immobilized gold nanoparticles for intelligent packaging. Colloids Surf B: Biointerfaces. 2019;176:439–48.

    Article  CAS  Google Scholar 

  22. Suh J, Paik HJ, Hwang BK. Ionization of poly(ethylenimine) and poly(allylamine) at various pH’s. Bioorg Chem. 1994;22(3):318–27.

    Article  CAS  Google Scholar 

  23. Kim K, Ryoo H, Lee YM, Shin KS. Adsorption characteristics of Au nanoparticles onto poly(4-vinylpyridine) surface revealed by QCM, AFM, UV/vis, and Raman scattering spectroscopy. J Colloid Interface Sci. 2010;342(2):479–84.

    Article  CAS  Google Scholar 

  24. Deligöz H, Tieke B. QCM-D study of layer-by-layer assembly of polyelectrolyte blend films and their drug loading-release behavior. Colloids Surf A Physicochem Eng Asp. 2014;441:725–36.

    Article  Google Scholar 

  25. Gu Y, Weinheimer EK, Ji X, Wiener CG, Zacharia NS. Response of swelling behavior of weak branched poly(ethylene imine)/poly(acrylic acid) polyelectrolyte multilayers to thermal treatment. Langmuir. 2016;32(24):6020–7.

    Article  CAS  Google Scholar 

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Acknowledgements

The authors would like to thank Aurélien Renaudin and Valentin Philippe from Université de Lorraine for their help with CZE and HPLC and Bernard Senger from INSERM U1121 for his help with the interpretation of the QCM data. The authors also acknowledge “Region Grand Est” for financial support.

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Authors and Affiliations

  1. Université de Lorraine, CITHEFOR, 54000, Nancy, France

    Arnaud Pallotta, Igor Clarot, Jordan Beurton, Benjamin Creusot, Thomas Chaigneau & Ariane Boudier

  2. Institut National de la Santé et de la Recherche Médicale, INSERM Unité 1121, 11 rue Humann, 67085, Strasbourg Cedex, France

    Jordan Beurton, Annie Tu & Philippe Lavalle

  3. Faculté de Chirurgie Dentaire, Université de Strasbourg, 8 rue Sainte Elisabeth, 67000, Strasbourg, France

    Annie Tu & Philippe Lavalle

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  1. Arnaud Pallotta
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  2. Igor Clarot
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  3. Jordan Beurton
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  8. Ariane Boudier
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Correspondence to Ariane Boudier.

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Pallotta, A., Clarot, I., Beurton, J. et al. Analytical strategy for studying the formation and stability of multilayered films containing gold nanoparticles. Anal Bioanal Chem 413, 1473–1483 (2021). https://doi.org/10.1007/s00216-020-03113-6

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  • DOI: https://doi.org/10.1007/s00216-020-03113-6

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