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Selective, sensitive, and fast determination of S-layer proteins by a molecularly imprinted photonic polymer coated film and a fiber-optic spectrometer

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Abstract

A newly developed molecularly imprinted photonic polymer (MIPP) film, which was prepared by colloidal crystal templating and surface molecular imprinting, was used for selective capture of S-layer protein (SLP) from a complex Lactobacillus acidophilus sample. The colloidal crystal templates were formed by a dipping process followed by chemical binding of the imprinted template SLP molecules. A sandwich structure consisting of two glass slides was formed after the SLP–silica layer had been covered with a poly(methyl methacrylate) glass slide. After polymerization of the SLP–silica layer with the preprepared polymerization solution, hydrofluoric acid and acetic phosphate buffer solutions removed the silica particles and SLP molecules, respectively. The MIPP film obtained exhibited a three-dimensional, highly ordered and interconnected macroporous structure (pore size greater than 200 nm), which is specifically accessible to SLP molecules. The adsorbed SLP molecules were simply and straightforwardly detected by a fiber-optic spectrometer. The redshift of the Bragg diffraction peak of the MIPP film was linearly related to the number of SLP molecules that had been harvested in the film. The detection limit of the SLP–MMIP–fiber-optic spectrometer method for SLP was 1 ng mL-1. The MIPP sensor was successfully applied to detect SLP molecules in a crudely extracted Lactobacillus acidophilus sample. Our results prove the applicability of the SLP–MIPP film for fast and real-time measurement of SLP.

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Acknowledgements

This work was supported by Natural Science Funding of China (31671869, 31471598, 31571852, and 31601487), Natural Science Funding of Jiangsu (BK20151544), and Natural Science Funding of Zhejiang (LQ16C200002).

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Authors and Affiliations

  1. State Key Laboratory for Quality and Safety of Agro-products, Ningbo University, Ningbo, Zhejiang, 315211, China

    Daodong Pan

  2. School of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210023, Jiangsu, China

    Daodong Pan, Mingyue Xun, Jianlin Li & Yuxing Guo

  3. Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Ningbo University, Ningbo, Zhejiang, 315800, China

    Daodong Pan, Hangzhen Lan & Zhen Wu

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  1. Daodong Pan
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Correspondence to Daodong Pan.

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Pan, D., Xun, M., Lan, H. et al. Selective, sensitive, and fast determination of S-layer proteins by a molecularly imprinted photonic polymer coated film and a fiber-optic spectrometer. Anal Bioanal Chem 411, 7737–7745 (2019). https://doi.org/10.1007/s00216-019-02109-1

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