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Dopamine-functionalized cyclodextrins: modification of reduced graphene oxide based electrodes and sensing of folic acid in human serum

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Abstract

A mandatory step in any sensor fabrication is the introduction of analyte-specific recognition elements to the transducer surface. In this study, the possibility to anchor β-cyclodextrin-modified dopamine to a reduced graphene oxide based electrochemical transducer for the sensitive and selective sensing of folic acid is demonstrated. The sensor displays good electrocatalytic activity toward the oxidation of folic acid. The strong affinity of the surface-confined β-cyclodextrin for folic acid, together with favorable electron transfer characteristics, resulted in a sensor with a detection limit of 1 nM for folic acid and a linear response up to 10 μM. Testing of the sensor on serum samples from healthy individuals and patients diagnosed with folic acid deficiency validated the sensing capability.

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Acknowledgements

Financial support from the Centre National de la Recherche Scientifique, the University of Lille, the Hauts-de-France region, the CPER “Photonics for Society,” and the Agence Nationale de la Recherche through the FLAG-ERA JTC 2015-Graphtivity project is acknowledged. We are grateful to the Walloon Region for financial support in the frame of the "BATWAL" project - "Programme d'Excellence". Work at UCLouvain has been carried out within the project LUMINOPTEX (avec le soutien du Fonds Européen de Développement Régional / met steun van het Europees Fonds voor Regionale Ontwikkeling - INTERREG V France-Wallonie-Vlaanderen). The work was supported by the Belgian F.R.S. - FNRS in the frame of the research conventions no. R 50.02.16.F and no. T.0106.16.

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Authors and Affiliations

  1. Department of Chemistry, Ayatollah Amoli Branch, Islamic Azad University, Amol, 46178-678, Iran

    Fereshteh Chekin

  2. Université de Lille, CNRS, Centrale Lille, ISEN, Université de Valenciennes, UMR 8520-IEMN, 59000, Lille, France

    Fereshteh Chekin, Vladyslav Mishyn, Alexandre Barras, Rabah Boukherroub & Sabine Szunerits

  3. Unité des Matériaux et Transformations (UMR 8207), Equipe Ingénierie des Systèmes Polymères, Université de Lille, 59655, Villeneuve d’Ascq Cedex, France

    Joel Lyskawa & Patrice Woisel

  4. Institute of Information and Communication Technologies, Electronics and Applied Mathematics, Université catholique de Louvain, 1348, Louvain-la-Neuve, Belgium

    Ran Ye & Sorin Melinte

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  1. Fereshteh Chekin
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Chekin, F., Mishyn, V., Barras, A. et al. Dopamine-functionalized cyclodextrins: modification of reduced graphene oxide based electrodes and sensing of folic acid in human serum. Anal Bioanal Chem 411, 5149–5157 (2019). https://doi.org/10.1007/s00216-019-01892-1

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