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A new SE-HPLC method for simultaneous quantification of proteins and main phenolic compounds from sunflower meal aqueous extracts

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Abstract

The aim of this research was to develop a method for simultaneous quantification of proteins and main polyphenolic compounds extracted from oleaginous meal by aqueous media. Size exclusion chromatography with a Biosep column (exclusion range from 1 to 300 kDa) and acetonitrile/water/formic acid (10:89.9:0.1 v/v) eluent at 0.6 mL min−1 yielded the most efficient separation of sunflower proteins and chlorogenic acid monoisomers (3-caffeoylquinic acid, 5-caffeoylquinic acid, and 4-caffeoylquinic acid). After a study of the stability of the extract components, the incorporation of a stabilization buffer (0.5 mol L−1 tris(hydroxymethyl)aminomethane-hydrochloric acid/1.0 mol L−1 sodium chloride at pH 7) was proposed to avoid polyphenol-protein interactions and/or isomeric transformation. The use of 214 nm as the wavelength for protein quantification was also included to minimize the effect of interference from polyphenol-protein interactions on the quantification. Under the used experimental conditions, the protein and chlorogenic acid monoisomer signals remained stable during 300 min at 20 °C (95–125% of the starting value). The developed method was validated and parameters such as specificity, sensitivity, precision, and accuracy were determined. The results from size exclusion chromatography correlated well with the results of protein determination by the reference Kjeldahl method. The proposed method was successfully applied for rapeseed extract analysis making simultaneous quantification of proteins and major rapeseed polyphenols (sinapine and sinapic acid) possible.

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References

  1. Gonzalez-Perez S. Sunflower proteins: overview of their physicochemical, structural and functional properties. J Sci Food Agric. 2007;87:2173–91.

    Article  CAS  Google Scholar 

  2. Wildermuth S. Chlorogenic acid oxidation and its reaction with sunflower proteins to form green-colored complexes. Compr Rev Food Sci Food Saf. 2016;15:829–43.

    Article  CAS  Google Scholar 

  3. Pickardt C. Optimisation of mild-acidic protein extraction from defatted sunflower (Helianthus annuus L.) meal. Food Hydrocoll. 2009;23:1966–73.

    Article  CAS  Google Scholar 

  4. Bongartz V. Evidence for the formation of benzacridine derivatives in alkaline-treated sunflower meal and model solutions. Molecules. 2016;21:91.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Ivanova P. Functional properties of proteins isolated from industrially produced sunflower meal. Int J Food Sci. 2014;3:203–12.

    Google Scholar 

  6. Pickardt C. Pilot plant preparation of light-coloured protein isolates from de-oiled sunflower (Helianthus annuus L.) press cake by mild-acidic protein extraction and polyphenol adsorption. Food Hydrocoll. 2015;44:208–19.

    Article  CAS  Google Scholar 

  7. Gueguen J. Plant proteins: context and potentialities for human food. Cahiers de nutrition et de dietetique. 2016;51:177–85.

    Article  Google Scholar 

  8. Aider M. Canola proteins: composition, extraction, functional properties, bioactivity, applications as a food ingredient and allergenicity - a practical and critical review. Trends Food Sci Technol. 2011;22:21–39.

    Article  CAS  Google Scholar 

  9. Tan SH. Canola proteins for human consumption: extraction, profile, and functional properties. J Food Sci. 2011;76:16–28.

    Article  CAS  Google Scholar 

  10. Gonzalez-Perez S. Isolation and characterization of undenatured chlorogenic acid free sunflower (Helianthus annuus) proteins. J Agric Food Chem. 2002;50:1713–9.

    Article  CAS  PubMed  Google Scholar 

  11. Ozdal T. A review on protein-phenolic interactions and associated changes. Food Res Int. 2013;51:954–70.

    Article  CAS  Google Scholar 

  12. Sapan CV. Colorimetric protein assay techniques. Anal Biochem. 1999;29:99–108.

    CAS  Google Scholar 

  13. Reichelt WN. Bioprocess monitoring: minimizing sample matrix effects for total protein quantification with bicinchoninic acid assay. Anal Biochem. 2016;43:1271–80.

    CAS  Google Scholar 

  14. Winters AL. Modification of the Lowry assay to measure proteins and phenols in covalently bound complexes. Anal Biochem. 2005;346:43–8.

    Article  CAS  PubMed  Google Scholar 

  15. Weisz GM. Identification and quantification of phenolic compounds from sunflower kernels and shells by HPLC-DAD/ESI-MSn. LWT Food Chem. 2009;115:758–65.

    Article  CAS  Google Scholar 

  16. Pedrosa M. Determination of caffeic and chlorogenic acids and their derivatives in different sunflower seed. J Sci Food Agric. 2000;80:459–64.

    Article  CAS  Google Scholar 

  17. Li J. Identification and quantification of phenolic compounds in rapeseed originated lecithin and antioxidant activity evaluation. LWT Food Sci Technol. 2016;73:397–405.

    Article  CAS  Google Scholar 

  18. ICH I. Q2 (R1): Validation of analytical procedures: text and methodology. In: International Conference on Harmonization, Geneva. 2005.

  19. Clifford MN. Chlorogenic acids and other cinnamates - nature, occurrence, dietary burden, absorption and metabolism. J Sci Food Agric. 2000;80:1033–43.

    Article  CAS  Google Scholar 

  20. AOCS International. Official Method 991.20. Nitrogen (total) in milk. Official methods of analysis, 19th edn. Gaithersburg: AOCS International; 1995.

  21. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227:680–5.

    Article  CAS  Google Scholar 

  22. Friedman M. Effect of pH on the stability of plant phenolic compounds. J Agric Food Chem. 2000;48:2101–10.

    Article  CAS  PubMed  Google Scholar 

  23. Xue M. Stability and degradation of caffeoylquinic acids under different storage conditions studied by high-performance liquid chromatography with photo diode array detection and electrospray ionization mass spectrometry. Molecules. 2016;21:948.

    Article  CAS  PubMed Central  Google Scholar 

  24. Yusaku N. Degradation kinetics of chlorogenic acid at various pH values and effects of ascorbic acid and epigallocatechin gallate on its stability under alkaline conditions. J Agric Food Chem. 2013;61:966–72.

    Article  CAS  Google Scholar 

  25. Deshpande S. Investigation of acyl migration in mono- and di-caffeoylquinic acids under aqueous basic, aqueous acidic, and dry roasting conditions. J Agric Food Chem. 2014;62:9160–70.

    Article  CAS  PubMed  Google Scholar 

  26. Hong P. A review size exclusion chromatography for the analysis of protein biotherapeutics and their aggregates. J Liq Chromatogr Relat Technol. 2012;35:2923–50.

    Article  CAS  Google Scholar 

  27. Ivanova P. Optimization of protein extraction from sunflower meal produced in Bulgaria. Bulg J Agric Sci. 2012;18:153–60.

    Google Scholar 

  28. Weisz GM. Sustainable sunflower processing - II. Recovery of phenolic compounds as a by-product of sunflower protein extraction. Innov Food Sci Emerg Technol. 2013;17:169–79.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratoire Réactions et Génie des Procédés, Université de Lorraine, CNRS, LRGP, F-54000, Nancy, France

    Sara Albe Slabi, Christelle Mathé, Xavier Framboisier, Claire Defaix, Odile Mesieres & Romain Kapel

  2. Avril Group, 75008, Paris, France

    Sara Albe Slabi, Claire Defaix & Olivier Galet

Authors
  1. Sara Albe Slabi
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  2. Christelle Mathé
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  3. Xavier Framboisier
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  4. Claire Defaix
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  5. Odile Mesieres
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  7. Romain Kapel
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Correspondence to Romain Kapel.

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Albe Slabi, S., Mathé, C., Framboisier, X. et al. A new SE-HPLC method for simultaneous quantification of proteins and main phenolic compounds from sunflower meal aqueous extracts. Anal Bioanal Chem 411, 2089–2099 (2019). https://doi.org/10.1007/s00216-019-01635-2

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  • DOI: https://doi.org/10.1007/s00216-019-01635-2

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