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A MnO2 nanosheet-based ratiometric fluorescent nanosensor with single excitation for rapid and specific detection of ascorbic acid

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Abstract

Ascorbic acid (AA) detection in biological sample and food sample is critical for human health. Herein, a MnO2 nanosheet (MnO2-NS)-based ratiometric fluorescent nanosensor has been developed for high sensitive and specific detection of AA. The MnO2-NS presents peroxidase-like activity and can oxidize non-fluorescent substrate of o-phenylenediamine (OPDA) into fluorescent substrate, presenting maximum fluorescence at 568 nm (F568). If MnO2-NS is premixed with AA, the MnO2-NS is then decomposed as Mn2+ by AA, decreasing the fluorescent intensity of F568. Meantime, AA is oxidized as dehydroascorbic acid (DHAA), which can react with OPDA to generate fluorescent substrate. A new fluorescence response is found at 425 nm (F425). The dual fluorescent responses can be excited with a universal excitation wavelength, simplifying the detection procedure. With F425/F568 as readout, limit of detection for AA reaches as low as 10.0 nM. Satisfactory recoveries are found for AA detection in serum and diverse beverages. The ratiometric strategy significantly eliminates false-negative and false-positive results, providing a cost-effective, rapid, and reliable way for AA detection in real sample.

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Funding

This work is supported by the National Natural Science Foundation of China (No. 21575138 and No. 21775108), the China International Science and Technology Cooperation Based of Food Nutrition/Safety and Medicinal Chemistry, the Tianjin Municipal Science and Technology Commission (Project No. 16PTSYJC00130), and the International Science and Technology Cooperation Program of China (Project No. 2014DFR30350).

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Authors and Affiliations

  1. State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety (Ministry of Education), College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China

    Yanlong Lyu, Zhanhui Tao, Xiaodong Lin, Pengcheng Qian, Yunfei Li & Yaqing Liu

  2. Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin, 300071, China

    Shuo Wang

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  1. Yanlong Lyu
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  2. Zhanhui Tao
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  6. Shuo Wang
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  7. Yaqing Liu
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Correspondence to Shuo Wang or Yaqing Liu.

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Published in the topical collection New Insights into Analytical Science in China with guest editors Lihua Zhang, Hua Cui, and Qiankun Zhuang.

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Lyu, Y., Tao, Z., Lin, X. et al. A MnO2 nanosheet-based ratiometric fluorescent nanosensor with single excitation for rapid and specific detection of ascorbic acid. Anal Bioanal Chem 411, 4093–4101 (2019). https://doi.org/10.1007/s00216-018-1439-2

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  • DOI: https://doi.org/10.1007/s00216-018-1439-2

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