Analytical and Bioanalytical Chemistry

, Volume 410, Issue 29, pp 7711–7721 | Cite as

Chromatographic study of nucleoside-lipids by RP-UHPLC-DAD/CAD

  • Ludivine Ferey
  • Sara Albe Slabi
  • Claude-Eric Roy
  • Philippe Barthelemy
  • Karen GaudinEmail author
Research Paper


Today, one of the most popular strategies in drug delivery is the encapsulation of therapeutic agents in supramolecular nanosystems formed from amphiphilic molecules. Synthetic nucleoside-lipids, composed of one nucleoside and lipidic chains, constitute promising new amphiphilic excipients under research in the field of pharmaceutical and biomedical applications. The aim of this work was to study the chromatographic behavior of these nucleoside-lipids in reversed-phase HPLC to establish appropriate chromatographic conditions for their analysis in drug delivery systems. The effect of the stationary phase, the organic solvent, the pH* values, and pH modifier nature of the mobile phase were studied on retention, peak shape, and detection. Good chromatographic performance was achieved on both Syncronis® C18 and Acquity® BEH C18 with mobile phases composed of MeOH/water, 95:5 (v/v) mixtures at apparent pH above 5. Dual detection by diode array detection (DAD) and charged aerosol detection (CAD) was investigated. CAD signal was found to be dependent on the type of pH modifiers added to the mobile phase. In isocratic elution, the same order of magnitude of CAD responses was obtained for the tested nucleoside-lipids. This study led to suitable chromatographic conditions for purity and stability studies of nucleoside-lipids. The purity of the synthetized molecules was established to be superior to 98%. Different stability in organic solvents was noticed depending on nucleoside-lipid structure. This first study will allow quantitative applications to establish loading ratio and encapsulation yield in future drug delivery systems composed of nucleoside-lipid-based assemblies.


Amphiphiles Charged aerosol detector Diode array detector Reversed-phase UHPLC 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Ludivine Ferey
    • 1
  • Sara Albe Slabi
    • 1
  • Claude-Eric Roy
    • 1
  • Philippe Barthelemy
    • 1
  • Karen Gaudin
    • 1
    Email author
  1. 1.ChemBioPharmARNA LaboratoryBordeauxFrance

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