Analytical and Bioanalytical Chemistry

, Volume 410, Issue 29, pp 7575–7589 | Cite as

Versatile nano-platform for tailored immuno-magnetic carriers

  • Emanuela Bonaiuto
  • Massimiliano Magro
  • Luca Fasolato
  • Enrico Novelli
  • Saeed Shams
  • Alessandra Piccirillo
  • Bita Bakhshi
  • Tahereh Tohidi Moghadam
  • Davide Baratella
  • Fabio VianelloEmail author
Paper in Forefront


Custom immuno-magnetic devices are desirable tools for biomedical and biotechnological applications. Herein, surface active maghemite nanoparticles (SAMNs) are proposed as a versatile platform for developing tailored immuno-magnetic nano-carriers by simple wet reactions. Two examples for conjugating native and biotinylated antibodies were presented along with their successful applications in the recognition of specific foodborne pathogens. Nanoparticles were functionalized with rhodamine B isothiocyanate (RITC), leading to a fluorescent nano-conjugate, and used for binding anti-Campylobacter fetus antibodies (SAMN@RITC@Anti-Cf). The microorganism was selectively captured in the presence of two other Campylobacter species (C. jejuni and C. coli), as verified by PCR. Alternatively, SAMNs were modified with avidin, forming a biotin-specific magnetic nano-carrier and used for the immobilization of biotinylated anti-Listeria monocytogenes antibodies (SAMN@avidin@Anti-Lm). This immuno-magnetic carrier was integrated in piezoelectric quartz crystal microbalance (QCM) sensor for the detection of L. monocytogenes in milk, showing a detection limit of 3 bacterial cells. The present work presents a new category of customized immuno-magnetic nano-carriers as a competitive option for suiting specific applications.

Graphical abstract


Immuno-magnetic separation Magnetic nano-carrier Antibody conjugation Campylobacter fetus Listeria monocytogenes Pathogen recognition 



Agar Listeria Ottavani and Agosti medium


Anchored piezoelectric-excited millimeter-sized cantilever


Capture efficiency


Campylobacter fetus


Colony-forming units


N-Cyclohexyl-N′-(2-morpholinoethyl) carbodiimide metho-p-toluenesulfonate




Enzyme-linked immunosorbent assay


Fourier transform infrared spectroscopy


Listeria monocytogenes


Limit of detection




Optical density


Phosphate-buffered saline


Plate count agar


Polymerase chain reaction


Quartz crystal microbalance


Rhodamine B isothiocyanate


Surface active maghemite nanoparticles


Transmission electron microscope


Tetramethylammonium perchlorate


Tryptone soya agar


Tryptone soya broth


X-ray powder diffraction



The authors thank the electron microscopy facility of the Biology Department of Padua University and the CARIPARO Foundation for the support.

Funding information

The present experimental work was partially funded by AIPOL Reg. CE n. 867/08 S.M.I, project “Miglioramento delle condizioni di magazzinaggio e di valorizzazione dei residui della produzione di olio di oliva e delle olive da tavola” approved by the Italian Ministry for Agricolture and Forest Politics and AGEA.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Emanuela Bonaiuto
    • 1
  • Massimiliano Magro
    • 1
    • 2
  • Luca Fasolato
    • 1
  • Enrico Novelli
    • 1
  • Saeed Shams
    • 1
    • 3
  • Alessandra Piccirillo
    • 1
  • Bita Bakhshi
    • 4
  • Tahereh Tohidi Moghadam
    • 5
  • Davide Baratella
    • 1
  • Fabio Vianello
    • 1
    Email author
  1. 1.Department of Comparative Biomedicine and Food ScienceUniversity of PaduaLegnaroItaly
  2. 2.Regional Centre of Advanced Technologies and MaterialsPalacký UniversityOlomoucCzech Republic
  3. 3.Cellular and Molecular Research CenterQom University of Medical SciencesQomIran
  4. 4.Department of Medical Bacteriology, Faculty of Medical SciencesTarbiat Modares UniversityTehranIran
  5. 5.Department of Nanobiotechnology, Faculty of Biological SciencesTarbiat Modares UniversityTehranIran

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