Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry
In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60 nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling.
KeywordsDot blot Antibody labelling Gold nanoparticles LA-ICP-MS Immunochemistry
Financial support was provided by Grant Agency of Czech Republic (GACR 17-12774S) and project CEITEC 2020 (LQ1601) with financial support from the Ministry of Education, Youth and Sports of the Czech Republic under the National Sustainability Program II. LPV was supported by project 6SA17676 that received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie and which is co-financed by the South Moravian Region under the grant agreement No. 665860.
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Conflict of interest
The authors declare that they have no conflict of interest.
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