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Analytical and Bioanalytical Chemistry

, Volume 410, Issue 20, pp 4805–4813 | Cite as

Colorimetric determination of glutathione in human serum and cell lines by exploiting the peroxidase-like activity of CuS-polydopamine-Au composite

  • Yanying Wang
  • Yaqin Liu
  • Fang Ding
  • Xiaoyan Zhu
  • Li Yang
  • Ping Zou
  • Hanbing Rao
  • Qingbiao Zhao
  • Xianxiang Wang
Research Paper

Abstract

In this study, we developed a simple colorimetric approach to detect glutathione (GSH). The proposed approach is based on the ability of CuS-PDA-Au composite material to catalytically oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to ox-TMB to induce a blue color with an absorption peak centered at 652 nm. However, the introduction of GSH can result in a decrease in oxidized TMB; similarly, it can combine with Au nanoparticles (Au NPs) on the surface of CuS-PDA-Au composite material. Both approaches can result in a fading blue color and a reduction of the absorbance at 652 nm. Based on this above, we proposed a technique to detect GSH quantitatively and qualitatively through UV-Vis spectroscopy and naked eye, respectively. This approach demonstrates a low detection limit of 0.42 μM with a broad detection range of 5 × 10−7–1 × 10−4 M with the assistance of UV-Vis spectroscopy. More importantly, this approach is convenient and rapid. This method was successfully applied to GSH detection in human serum and cell lines.

Graphical abstract

A colorimetric approach has been developed by exploiting the peroxidase-like activity of CuS-polydopamine-Au composite for sensitive glutathione detection.

Keywords

Glutathione CuS-PDA-Au composite material Peroxidase-like activity Cancer cells 

Notes

Acknowledgments

We thank the anonymous reviewers for their valuable suggestions.

Funding information

This work was supported by a grant from the National Natural Science Foundation of China (21305097) and Two-way Support Plan Foundation of Sichuan Agricultural University.

Compliance with ethical standards

The project was approved by the Third People’s Hospital of Chengdu. All experiments were performed in compliance with the relevant laws and institutional guidelines.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2018_1117_MOESM1_ESM.pdf (406 kb)
ESM 1 (PDF 406 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Yanying Wang
    • 1
  • Yaqin Liu
    • 1
  • Fang Ding
    • 2
    • 3
  • Xiaoyan Zhu
    • 1
  • Li Yang
    • 1
  • Ping Zou
    • 1
  • Hanbing Rao
    • 1
  • Qingbiao Zhao
    • 4
  • Xianxiang Wang
    • 1
  1. 1.College of ScienceSichuan Agricultural UniversityYa’anChina
  2. 2.Suzhou Institute of Systems MedicineSuzhouChina
  3. 3.Center for Systems Medicine, Institute of Basic Medical SciencesChinese Academy of Medical Sciences & Peking Union Medical CollegeBeijingChina
  4. 4.Key Laboratory of Polar Materials and Devices, Ministry of Education, Department of Electronic EngineeringEast China Normal UniversityShanghaiChina

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