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Analytical and Bioanalytical Chemistry

, Volume 410, Issue 14, pp 3315–3323 | Cite as

Precise, accurate and user-independent blood collection system for dried blood spot sample preparation

  • Ricardo Neto
  • Andrew Gooley
  • Michael C. Breadmore
  • Emily F. Hilder
  • Florian Lapierre
Research Paper

Abstract

An accurate and precise 3 μL blood collection and dispensing system is presented for the preparation of dried blood spot (DBS) samples. Using end-to-end glass capillaries in conjugation with pre-punched DBS pads, a blood micro collection system was developed to eliminate the haematocrit dispersion, widely associated with DBS technology, while providing better levels of accuracy and precision during sample preparation. This methodology is compared to traditional micro-volume blood collection systems, such as a pipette and a digitally controlled analytical syringe. Results showed that % of recovery for the capillary methodology was closer to 100% across the three haematocrit (HCT) levels tested and when prepared by two users (98 to 100% for capillaries, 78 to 104% for pipette and 93 to 97% for digital syringe) attesting a higher accuracy. Additionally, by taking advantage of the capillary action mechanism to collect and dispense autonomously the desired volume of blood onto the DBS pad, coefficients of variation between two individuals were significantly lower than with standard methodologies (capillaries—0.05 to 0.77%, pipette—12.71 to 18.53% and digital syringe—0.72 to 1.77%). This alternate aspiration and dispensing methodology could be used by different users without compromising accuracy or precision when handling low volumes of blood during the pre-analytical steps.

Graphical abstract

Comparison of novel capillary dispensing methodology for dried blood spot sample preparation with pipette and digital syringe methodologies through accuracy and precision measurements of caffeine

Keywords

Dried blood spot Microsampling Glass capillary Blood 

Notes

Acknowledgements and Funding information

This research was conducted by the Australian Research Council (ARC) Training Centre for Portable Analytical Separation Technologies (IC140100022). MCB is a recipient of an ARC Future Fellowship (FT130100101). RN is a recipient of an ARC ICHDR scholarship and an International Research Tuition Scholarship from the University of South Australia. Support from the University of South Australia, University of Tasmania and Trajan Scientific and Medical is gratefully acknowledged.

Compliance with ethical standards

All volunteers signed a consent form stating their understanding of the experiments and protecting their privacy. Ethics approval was provided by the University of South Australia (application number: 0000036188) and by the University of Tasmania (reference number H0015476) from their respective ethics committees.

Conflict of interest

The authors declare the following competing financial interest(s): Andrew Gooley is an executive director of Trajan Scientific and Medical. Florian Lapierre is subject to intellectual property rights. All the other authors declare that they have no conflict of interest.

Supplementary material

216_2018_993_MOESM1_ESM.pdf (537 kb)
ESM 1 (PDF 536 kb)
216_2018_993_MOESM2_ESM.mp4 (5.3 mb)
ESM 2 (MP4 5457 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Future Industries InstituteUniversity of South AustraliaAdelaideAustralia
  2. 2.ARC Training Centre for Portable Analytical Separation Technologies (ASTech)HobartAustralia
  3. 3.Trajan Scientific and MedicalRingwoodAustralia
  4. 4.Australian Centre for Research on Separation Science, School of Natural SciencesUniversity of TasmaniaHobartAustralia

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