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Novel electrochemical aptasensor for ultrasensitive detection of sulfadimidine based on covalently linked multi-walled carbon nanotubes and in situ synthesized gold nanoparticle composites

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Abstract

In the current study, a sensitive electrochemical sensing strategy based on aptamer (APT) for detection of sulfadimidine (SM2) was developed. A bare gold electrode (AuE) was first modified with 2-aminoethanethiol (2-AET) through self-assembly, used as linker for the subsequent immobilization of multi-walled carbon nanotubes and gold nanoparticle composites (MWCNTs/AuNPs). Then, the thiolated APT was assembled onto the electrode via sulfur-gold affinity. When SM2 existed, the APT combined with SM2 and formed a complex structure. The specific binding of SM2 and APT increased the impedance, leading to hard electron transfer between the electrode surface and the redox probe [Fe(CN)6]3−/4− and producing a significant reduction of the signal. The SM2 concentration could be reflected by the current difference of the peak currents before and after target binding. Under optimized conditions, the linear dynamic range is from 0.1 to 50 ng mL−1, with a detection limit of 0.055 ng mL−1. The sensor exhibited desirable selectivity against other sulfonamides and performs successfully when analyzing SM2 in pork samples.

A new electrochemical biosensor for ultrasensitive detection of sulfadimidine (SM2) by using a gold electrode modified with MWCNTs/AuNPs for signal amplification and aptamer (APT) for selectivity improvement.

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Funding

This work was supported by the National Natural Science Foundation of China (Grant No. 61301037), Henan Science and Technology Cooperation Project (Grant No. 172106000014), Cultivation Plan for Young Core Teachers in Universities of Henan Province (No. 2017GGJS072), Foundation of Henan Educational Committee (Grant No. 13A550194), Fundamental Research Funds for the Henan Provincial Colleges and Universities (Grant No. 2014YWQQ05), and Youth Backbone Teacher Training Program of Henan University of Technology.

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  1. School of Food Science and Technology, Henan University of Technology, Lianhua Road 100#, Zhengzhou, Henan, 450001, China

    Baoshan He & Gengan Du

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  1. Baoshan He
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  2. Gengan Du
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Correspondence to Baoshan He.

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He, B., Du, G. Novel electrochemical aptasensor for ultrasensitive detection of sulfadimidine based on covalently linked multi-walled carbon nanotubes and in situ synthesized gold nanoparticle composites. Anal Bioanal Chem 410, 2901–2910 (2018). https://doi.org/10.1007/s00216-018-0970-5

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  • DOI: https://doi.org/10.1007/s00216-018-0970-5

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