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Establishment of pressurized-liquid extraction by response surface methodology approach coupled to HPLC-DAD-TOF-MS for the determination of phenolic compounds of myrtle leaves

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Abstract

Myrtus communis L. (myrtle) is native to the Mediterranean region and Western Asia. Its leaves have demonstrated its potential effect towards different bioactivities like anti-diabetic, anti-diarrheic, anti-ulcer, anti-cancer, among others. These activities have been associated with its phenolic content. In this sense, the aim of this work has been to develop a new pressurized-liquid extraction procedure (PLE), by using a response surface methodology (RSM), to evaluate the phenolic composition from myrtle leaves by HPLC-DAD-TOF-MS. Previously, different solvents such as methanol, ethanol, and acetone/water mixtures were tested by using ultrasound-assisted extraction (UAE) in order to select the most suitable one. Subsequently, a Box-Behnken design (BBD) was performed according to the effect of ethanol/water ratio (50, 75, and 100% (v/v)), temperature (50, 125, and 200 °C), and extraction time (5, 18, and 30 min). The optimal conditions achieved with the established method were 71% ethanol/water, 137 °C, and 19 min. The analysis of the obtained extracts by HPLC-DAD-TOF-MS allowed the characterization of 15 new compounds in myrtle leaves. Finally, high amounts of gallic and ellagic acid were found in the optimized PLE extracts (3.31 ± 0.03 and 3.88 ± 0.09 mg/g leaf dry weight (d.w.), respectively), and PLE reported greater recovery of total phenolic compounds than UAE (30 ± 1 and 22.4 ± 0.6 mg/g leaf d.w., respectively).

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Acknowledgements

This work was funded by projects AGL2015-67995-C3-2-R (Spanish Ministry of Science and Innovation), as well as by project P11-CTS-7625 (Andalusian Regional Government Council of Innovation and Science)

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avd. Fuentenueva s/n, 18071, Granada, Spain

    Elixabet Díaz-de-Cerio, David Arráez-Román, Antonio Segura-Carretero & Ana María Gómez-Caravaca

  2. Research and Development Functional Food Centre, Health Science Technological Park, Avd. del Conocimiento 36, Bioregion building, 18016, Granada, Spain

    Elixabet Díaz-de-Cerio, David Arráez-Román, Antonio Segura-Carretero & Ana María Gómez-Caravaca

  3. Department of Agricultural Sciences, University of Naples Federico II, Via Università, 100, 80055, Portici, NA, Italy

    Pasquale Ferranti, Rosario Nicoletti & Giuseppe Mirko Perrotta

  4. Consiglio per la Ricerca in Agricoltura e l’Analisi dell’Economia Agraria, Via Po, 14, 00198, Rome, Italy

    Rosario Nicoletti

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  1. Elixabet Díaz-de-Cerio
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Correspondence to Ana María Gómez-Caravaca.

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Published in the topical collection Discovery of Bioactive Compounds with guest editors Aldo Laganà, Anna Laura Capriotti and Chiara Cavaliere.

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Díaz-de-Cerio, E., Arráez-Román, D., Segura-Carretero, A. et al. Establishment of pressurized-liquid extraction by response surface methodology approach coupled to HPLC-DAD-TOF-MS for the determination of phenolic compounds of myrtle leaves. Anal Bioanal Chem 410, 3547–3557 (2018). https://doi.org/10.1007/s00216-018-0914-0

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