Establishment of pressurized-liquid extraction by response surface methodology approach coupled to HPLC-DAD-TOF-MS for the determination of phenolic compounds of myrtle leaves
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Myrtus communis L. (myrtle) is native to the Mediterranean region and Western Asia. Its leaves have demonstrated its potential effect towards different bioactivities like anti-diabetic, anti-diarrheic, anti-ulcer, anti-cancer, among others. These activities have been associated with its phenolic content. In this sense, the aim of this work has been to develop a new pressurized-liquid extraction procedure (PLE), by using a response surface methodology (RSM), to evaluate the phenolic composition from myrtle leaves by HPLC-DAD-TOF-MS. Previously, different solvents such as methanol, ethanol, and acetone/water mixtures were tested by using ultrasound-assisted extraction (UAE) in order to select the most suitable one. Subsequently, a Box-Behnken design (BBD) was performed according to the effect of ethanol/water ratio (50, 75, and 100% (v/v)), temperature (50, 125, and 200 °C), and extraction time (5, 18, and 30 min). The optimal conditions achieved with the established method were 71% ethanol/water, 137 °C, and 19 min. The analysis of the obtained extracts by HPLC-DAD-TOF-MS allowed the characterization of 15 new compounds in myrtle leaves. Finally, high amounts of gallic and ellagic acid were found in the optimized PLE extracts (3.31 ± 0.03 and 3.88 ± 0.09 mg/g leaf dry weight (d.w.), respectively), and PLE reported greater recovery of total phenolic compounds than UAE (30 ± 1 and 22.4 ± 0.6 mg/g leaf d.w., respectively).
KeywordsMyrtus communis L. Ultrasound-assisted extraction Pressurized liquid extraction Response surface methodology HPLC-DAD-TOF-MS Phenolic compounds
This work was funded by projects AGL2015-67995-C3-2-R (Spanish Ministry of Science and Innovation), as well as by project P11-CTS-7625 (Andalusian Regional Government Council of Innovation and Science)
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human or animal subjects.
Informed consent was not applicable.
- 1.Gençler Özkan AM, Gençler GÇ. A Mediterranean: Myrtus communis L. (Myrtle). In: Morel J-P, Mercuri AM, editors. Plants and culture: seeds of the cultural heritage of Europe. Bari: Edipuglia srl; 2009. p. 159–68.Google Scholar
- 2.Alipour G, Dashti S, Hosseinzadeh H. Review of pharmacological effects of Myrtus communis L. and its active constituents, 2014. Phyther Res. 1136:1125–36.Google Scholar
- 11.Rodríguez-Pérez C, Gilbert-López B, Mendiola JA, Quirantes-Piné R, Segura-Carretero A, Ibáñez E. Optimization of microwave-assisted extraction and pressurized liquid extraction of phenolic compounds from Moringa oleifera leaves by multiresponse surface methodology. Electrophoresis. 2016:1938–46.Google Scholar
- 14.Hossain MB, Barry-Ryan C, Martin-Diana AB, Brunton NP. Optimisation of accelerated solvent extraction of antioxidant compounds from rosemary (Rosmarinus officinalis L.), marjoram (Origanum majorana L.) and oregano (Origanum vulgare L.) using response surface methodology. Food Chem. 2011;126(1):339–46.CrossRefGoogle Scholar
- 15.Sarker SD, Nahar L. Hyphenated techniques and their applications in natural products analysis. In: Natural products isolation Methods and protocols. 2009. p. 75–88.Google Scholar
- 18.Miller JN, Miller JC. Statistics and chemometrics for analytical chemistry. Vol. 6th Ed, Prentice Hall. 2010.Google Scholar
- 19.Bruker Daltonics Technical Note #008. Molecular formula determination under automation.Google Scholar
- 20.Fatiha B, Khodir M, Farid D, Tiziri R, Karima B, Sonia O, et al. Optimisation of solvent extraction of antioxidants (phenolic compounds) from Algerian mint (Mentha spicata L.). Pharmacogn Commun. 2012;2(4):72–86.Google Scholar
- 22.Vihakas M. Flavonoids and other phenolic compounds: characterization and interactions with lepidopteran and sawfly larvae. Turun Yliopiston Julkaisuja – Annales Universitatis Turkuensis, 2014.Google Scholar
- 23.Tanaka T, Tong H-H, Xu Y-M, Ishimaru K, Nonaka G, Nishioka I. Tannins and related compounds. CXVII. Isolation and characterization of three new ellagitannins, Lagerstannins A, B and C, having a gluconic acid core, from Lagerstroemia speciosa (L.) Pers. Chem Pharm Bull (Tokyo). 1992;40(11):2975–80.CrossRefGoogle Scholar
- 24.Gallo MBC, Rocha WC, da Cunha US, Diogo FA, da Silva FC, Vieira PC, et al. Bioactivity of extracts and isolated compounds from Vitex polygama (Verbenaceae) and Siphoneugena densiflora (Myrtaceae) against Spodoptera frugiperda (Lepidoptera: Noctuidae). Pest Manag Sci. 2006;62:1072–81.CrossRefPubMedGoogle Scholar
- 25.Tanaka T, Orii Y, Nonaka G, Nishioka I. Tannins and related compounds. CXXIII. Chromone, acetophenone and phenylpropanoid glycosides and their galloyl and/or hexahydroxydiphenoyl esters from the leaves of Syzygium aromaticum Merr. et Perry. Chem Pharm Bull (Tokyo). 1993;41(7):1232–7.CrossRefGoogle Scholar
- 29.Djoukeng JD, Abou-Mansour E, Tapondjou LA, Lontsi D, Tabacchi R. Identification of ellagic acid derivatives from stem bark of Syzygium guineense (Myrtaceae). Nat Prod Commun. 2007;2:1–6.Google Scholar
- 34.Jamil DAK. Preliminary phytochemical and screening of biocomponents by GC-MS technique in Myrtus communis L. plant flowers. AL-Qadisiyha J Pure Sci. 2016;1(21):23–33.Google Scholar
- 37.Varughese T, Rahaman M, Kim N, Cho S, Moon S. Gamnamoside, a phenylpropanoid glycoside from persimmon leaves (Diospyros kaki) with an inhibitory effect against an alcohol metabolizing enzyme. Bull Kor Chem Soc. 2009;30(5):1–4.Google Scholar
- 40.Pfundstein B, El Desouky SK, Hull WE, Haubner R, Erben G, Owen RW. Polyphenolic compounds in the fruits of Egyptian medicinal plants (Terminalia bellerica, Terminalia chebula and Terminalia horrida): characterization, quantitation and determination of antioxidant capacities. Phytochemistry. 2010;71(10):1132–48.CrossRefPubMedGoogle Scholar