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Analytical and Bioanalytical Chemistry

, Volume 410, Issue 6, pp 1845–1854 | Cite as

Preparation and application of a molecularly imprinted monolith for specific recognition of domoic acid

  • Fan Yang
  • Ruirui Wang
  • Guangshui Na
  • Qilun Yan
  • Zhongsheng Lin
  • Zhifeng Zhang
Research Paper

Abstract

In this work, a molecularly imprinted monolithic column was synthesized by a facile procedure and was applied for specific recognition of domoic acid, an amnesic shellfish poison. The poly(4-vinylpyridine-co-ethylene glycol dimethacrylate) molecularly imprinted monolith was synthesized in a stainless steel column by in situ polymerization. Pentane-1,3,5-tricarboxylic acid was used as a dummy imprinting template instead of the highly toxic and expensive target molecule. It is the first time that a molecularly imprinted monolith is introduced for separation and detection of domoic acid. After optimizing the preparation conditions, the prepared imprinted monolith was systematically characterized and exhibited excellent stability and permeability as a HPLC stationary phase. The results of chromatographic analysis demonstrated that the molecularly imprinted monolith exhibited specific retention and selective recognition toward domoic acid, with an imprinted factor up to 3.77. Furthermore, the molecularly imprinted monolith was successfully applied for selective enrichment of domoic acid from biological samples.

Graphical abstract

A molecularly imprinted monolith was prepared by using a dummy imprinted template and was successfully applied for specific recognition of domoic acid.

Keywords

Molecular imprinting Monolith Domoic acid High performance liquid chromatography 

Notes

Funding Information

The authors are grateful to the support of National Natural Science Foundation of China (No 21405026).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Fan Yang
    • 1
  • Ruirui Wang
    • 1
  • Guangshui Na
    • 1
  • Qilun Yan
    • 1
  • Zhongsheng Lin
    • 1
  • Zhifeng Zhang
    • 1
  1. 1.Key Laboratory for Ecological Environment in Coastal Areas (SOA), National Marine Environmental Monitoring CenterDalianChina

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