Analytical and Bioanalytical Chemistry

, Volume 409, Issue 14, pp 3573–3585 | Cite as

Development of an enzymatic reactor applying spontaneously adsorbed trypsin on the surface of a PDMS microfluidic device

  • Adam Kecskemeti
  • Jozsef Bako
  • Istvan Csarnovics
  • Eva Csosz
  • Attila Gaspar
Research Paper

Abstract

Herein, a microfluidic device (MD) containing immobilized trypsin for rapid and efficient proteolysis was described. Trypsin was immobilized via non-specific protein adsorption onto the hydrophobic poly(dimethylsiloxane) (PDMS) channel wall of the MD. Peptide mapping of bovine serum albumin (BSA) samples was carried out to estimate the stability of trypsin adsorbed on PDMS surface. Peptide maps of BSA samples were obtained by capillary zone electrophoresis (CZE), the RSD% for migration times were under 1%. Several proteins (hemoglobin, myoglobin, lysozyme, and BSA) in a wide molecular size range (15–70 kDa) were digested efficiently with ∼50 s contact time. The number of separated peaks correlated well with the expected number of peptides formed in the complete tryptic digestion of the proteins. Peptide mass fingerprinting of BSA and human serum was carried out. Trypsin retained its activity for 2 h; within this period, the MD can be used for multiple digestions. The main properties of this device are simple channel pattern, simple immobilization procedure, regenerability, and disposability; all these features make this MD one of the simplest yet applicable enzymatic microreactors.

Graphical abstract

Development of microfluidic device including a serpentine channel as an enzyme reactor for protein digestion.

Keywords

Immobilization Trypsin Digestion Peptide mapping Enzyme reactor Microfluidic device 

Notes

Acknowledgements

The research was supported by the EU and co-financed by the European Regional Development Fund under the project GINOP-2.3.2-15-2016-00008, GINOP-2.3.3-15-2016-00004, and NTP-EFÖ-P-15-0003 project. The authors also acknowledge the financial support provided to this project by the National Research, Development and Innovation Office, Hungary (K111932). We would like to thank Prof. Dr. Csaba Hegedűs for measurement possibilities in the Biomaterials Research Lab on the Faculty of Dentistry.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2017_295_MOESM1_ESM.pdf (162 kb)
ESM 1 (PDF 162 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Adam Kecskemeti
    • 1
  • Jozsef Bako
    • 2
  • Istvan Csarnovics
    • 3
  • Eva Csosz
    • 4
  • Attila Gaspar
    • 1
  1. 1.Department of Inorganic and Analytical ChemistryUniversity of DebrecenDebrecenHungary
  2. 2.Department of Biomaterials and Prosthetic DentistryUniversity of DebrecenDebrecenHungary
  3. 3.Department of Experimental PhysicsUniversity of DebrecenDebrecenHungary
  4. 4.Department of Biochemistry and Molecular BiologyUniversity of DebrecenDebrecenHungary

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