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Electrochemiluminescence (ECL) immunosensor for detection of Francisella tularensis on screen-printed gold electrode array

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Abstract

An electrochemiluminescence (ECL) immunosensor for the rapid detection of the Francisella tularensis pathogen using whole antibodies or antibody fragments as capture biomolecule is described. A sandwich immunoassay was used with either lipopolysaccharide (LPS) or the whole inactivated bacterial cell (LVS) as a target, while Ru(bpy)3 2+-encapsulated silicate nanoparticles were linked to the secondary antibody and used as ECL labels. The assay was performed in a fluidic chip housed in a custom-built black box incorporating electronics, optics and fluidics. The obtained limit of detection for LPS was 0.4 ng/mL, while for the LVS it was 70 and 45 bacteria/mL when the capturing molecule was the whole antibody and the antibody F(ab) fragment, respectively.

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References

  1. Seo Y, Kim J, Jeong Y, Lee KH, Hwang J, Hong J, et al. Nanoscale. 2016;8:1944.

    Article  CAS  Google Scholar 

  2. Silvestri EE, Perkins SD, Rice EW, Stone H, Shaefer III FW. Anal Microbiol. 2016;66:77–89.

    Article  CAS  Google Scholar 

  3. Hua F, Zhang P, Zhang F, Zhao Y, Li C, Sun C, et al. Sci Rep. 2015;5:17178.

    Article  CAS  Google Scholar 

  4. Seo SH, Lee Y-R, Jeon JH, Hwang Y-R, Park P-G, Ahn D-R, et al. Biosens Bioelectron. 2015;64:69–73.

    Article  CAS  Google Scholar 

  5. Kleo K, Schafer D, Klar S, Jacob D, Grunow R, Lisdat F. Anal Bioanal Chem. 2012;404:843–51.

    Article  CAS  Google Scholar 

  6. Dulay S, Gransee R, Julich S, Tomaso H, O’Sullivan CK. Biosens Bioelectron. 2014;59:342–9.

    Article  CAS  Google Scholar 

  7. Lazcka O, Del Campo FJ, Munoz FX. Biosens Bioelectron. 2007;22:1205–17.

    Article  CAS  Google Scholar 

  8. Richter MM. Chem Rev. 2004;104:3003–36.

    Article  CAS  Google Scholar 

  9. Bertoncello P, Forster RJ. Biosens Bioelectron. 2009;24:3191–200.

    Article  CAS  Google Scholar 

  10. Muzyka K. Biosens Bioelectron. 2014;54:393–407.

    Article  CAS  Google Scholar 

  11. Blackburn GF, Shag HP, Kenten JH, Leland J, Kamin RA, Link J, et al. Clin Chem. 1991;37(9):1534–9.

    CAS  Google Scholar 

  12. Miao W, Bard AJ. Anal Chem. 2004;76(23):7109–13.

    Article  CAS  Google Scholar 

  13. Spehar-Délèze A, Gransee R, Martinez Montequin S, Bejarano D, Dulay S, Julich S, et al. Anal Bional Chem. 2015;407(22):6657–67.

    Article  Google Scholar 

  14. Zhang LH, Dong SJ. Anal Chem. 2006;78:5119–23.

    Article  CAS  Google Scholar 

  15. Sardesai NP, Barron J, Rusling JF. Anal Chem. 2011;83:6698–703.

    Article  CAS  Google Scholar 

  16. Qian J, Zhou Z, Cao X, Liu S. Anal Chim Acta. 2010;665:32–8.

    Article  CAS  Google Scholar 

  17. Morel N, Volland H, Dano J, Lamourette P, Sylvestre P, Mock M, et al. Appl Environ Microbiol. 2012;78(18):6491–8.

    Article  CAS  Google Scholar 

  18. Nassef HM, Civit L, Fragoso A, O’Sullivan CK. Anal Chem. 2009;81:5299–307.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work has been carried out with financial support from the Commission of the European Communities, RTD programme “The lab-free CBRN detection device for the identification of biological pathogens on nucleic acid and immunological level as lab-on-a-chip system applying multisensor technologies”, MultisenseChip [FP7-SEC-2010-1]. The authors thank microfluidic ChipShop (http://www.microfluidic-chipshop.com/) for provision of the microfluidics.

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Authors and Affiliations

  1. Departament d’Enginyeria Quimica, Universitat Rovira i Virgili, Avinguda Països Catalans 26, 43007, Tarragona, Spain

    Anna-Maria Spehar-Délèze, Samuel B. Dulay & Ciara K. O’Sullivan

  2. Fraunhofer ICT-IMM, Carl-Zeiss-Strasse 18-20, 55129, Mainz, Germany

    Rainer Gransee

  3. Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffer-Institut, Naumburger Str. 96A, 07743, Jena, Germany

    Sandra Julich & Herbert Tomaso

  4. Institució Catalana de Recerca i Estudis Avancats, Passeig Lluís Companys, 23, 08010, Barcelona, Spain

    Ciara K. O’Sullivan

Authors
  1. Anna-Maria Spehar-Délèze
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  2. Sandra Julich
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  3. Rainer Gransee
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  4. Herbert Tomaso
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  5. Samuel B. Dulay
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  6. Ciara K. O’Sullivan
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Corresponding authors

Correspondence to Anna-Maria Spehar-Délèze or Ciara K. O’Sullivan.

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The authors declare that they have no conflicts of interest.

Additional information

Published in the topical collection Analytical Electrochemiluminescence with guest editors Hua Cui, Francesco Paolucci, Neso Sojic, and Guobao Xu.

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Spehar-Délèze, AM., Julich, S., Gransee, R. et al. Electrochemiluminescence (ECL) immunosensor for detection of Francisella tularensis on screen-printed gold electrode array. Anal Bioanal Chem 408, 7147–7153 (2016). https://doi.org/10.1007/s00216-016-9658-x

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  • DOI: https://doi.org/10.1007/s00216-016-9658-x

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