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Improved electrochemiluminescence labels for heterogeneous microbead immunoassay

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Abstract

Ruthenium(II) complexes with carboxylic acid as a bioconjugatable group, i.e., [Ru(bathophenanthroline disulfonate)(2,2′-bipyridine)(4-methyl-4′-(3-carboxypropyl)-2,2′-bipyridine)]0, (C49H38N6O8S2Ru), and [Ru(bathophenanthroline disulfonate)2(4-methyl-4′-(3-carboxypropyl)-2,2′-bipyridine)]2− · 2Na+, (C63H44N6O14S4RuNa2) were characterized spectroscopically and electrochemically. As potential labels for electrochemiluminescence (ECL) immunoassays, the ECL intensities of the free labels in homogenous aqueous buffer solutions were compared under a condition that is similar to the one employed by a commercial clinical immunoassay system. The two labels were found to be more emissive and, thus, can be detected at 10- 12 pM compared with 5× 10−12 pM of the label currently used in the commercial ECL system. Furthermore, the improved ECL emission of the free labels in homogenous solutions was proven to be translated into more intense ECL signal in heterogeneous sandwich immunoassay and, thus, leading to a lower limit of detection in immunoassay. The data obtained from these ECL labels shed light on the further development of ECL-based clinical immunoassay technology.

Electrochemiluminescence immunoassays were carried out with three different ruthenium(II) complex labels. It was proved that the higher signal intensities found with the novel labels in homogeneous solutions were maintained in heterogeneous sandwich format.

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Authors and Affiliations

  1. SunaTech Inc., bioBAY, Suzhou Industrial Park, Suzhou, Jiangsu, 215123, China

    Linpo Yu & Yang Liu

  2. Rubipy Scientific Inc., 880 Taylor Creek Drive, Ottawa, Ontario, K1C 1T1, Canada

    Ming Zhou

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  1. Linpo Yu
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  2. Yang Liu
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  3. Ming Zhou
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Correspondence to Ming Zhou.

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Published in the topical collection Analytical Electrochemiluminescence with guest editors Hua Cui, Francesco Paolucci, Neso Sojic, and Guobao Xu.

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Yu, L., Liu, Y. & Zhou, M. Improved electrochemiluminescence labels for heterogeneous microbead immunoassay. Anal Bioanal Chem 408, 7095–7103 (2016). https://doi.org/10.1007/s00216-016-9583-z

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