Analytical and Bioanalytical Chemistry

, Volume 408, Issue 10, pp 2383–2406 | Cite as

Oligonucleotide-based biosensors for in vitro diagnostics and environmental hazard detection

  • Il Young Jung
  • Eun Hee Lee
  • Ah Young Suh
  • Seung Jin Lee
  • Hyukjin LeeEmail author
Part of the following topical collections:
  1. ABC Highlights: authored by Rising Stars and Top Experts


Oligonucleotide-based biosensors have drawn much attention because of their broad applications in in vitro diagnostics and environmental hazard detection. They are particularly of interest to many researchers because of their high specificity as well as excellent sensitivity. Recently, oligonucleotide-based biosensors have been used to achieve not only genetic detection of targets but also the detection of small molecules, peptides, and proteins. This has further broadened the applications of these sensors in the medical and health care industry. In this review, we highlight various examples of oligonucleotide-based biosensors for the detection of diseases, drugs, and environmentally hazardous chemicals. Each example is provided with detailed schematics of the detection mechanism in addition to the supporting experimental results. Furthermore, future perspectives and new challenges in oligonucleotide-based biosensors are discussed.


Biosensors Oligonucelotide In vitro diagnosis Environmental harzard detection 


Amyloid β


Aptamer-coated nanogold


Alzheimer’s disease


Amyloid β derived diffusible ligand


Gold nanoparticle


Nanoparticle consisting of a gold core and a platinum shell


Bisphenol A


Complementary DNA


Carcinoembryonic antigen


Cerebrospinal fluid


Endocrine-disrupting compound


Fluorescence resonance energy transfer




Hepatitis B virus


Hyperbranched rolling circle amplification


Horseradish peroxidase


Localized surface plasmon resonance


Gold nanoparticles with magnetic microbeads inside


Multibranched hybridization chain reaction


Multiwalled carbon nanotube


Nanoporous gold


Ochratoxin A


Polymerase chain reaction


Platelet-derived growth factor


Cellular prion protein


Quartz crystal microbalance


Quantum dot


Rolling circle amplification


Reverse transcription polymerase chain reaction


Systemic evolution of ligands by exponential enrichment


Surface-enhanced resonance Raman scattering


Single-stranded DNA




Triple-helix molecular switch



I.Y.J. and E.H.L and A.Y.S. contributed equally to this work. This work was supported by the Basic Science Research Program (2012R1A1A1A05027352), The National Research Foundation of South Korea: BioMedical Technology Development Program (2015M3A9D7031026), and the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Science, ICT & Future Planning (NRF-2013K2A2A6000467)

Compliance with ethical standard

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Il Young Jung
    • 1
  • Eun Hee Lee
    • 1
  • Ah Young Suh
    • 1
  • Seung Jin Lee
    • 1
  • Hyukjin Lee
    • 1
    Email author
  1. 1.College of Pharmacy, Graduate School of Pharmaceutical SciencesEwha Womans UniversitySeoulRepublic of Korea

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