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Characterization of Dickeya and Pectobacterium species by capillary electrophoretic techniques and MALDI-TOF MS

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Abstract

Dickeya and Pectobacterium species represent an important group of broad-host-range phytopathogens responsible for blackleg and soft rot diseases on numerous plants including many economically important plants. Although these species are commonly detected using cultural, serological, and molecular methods, these methods are sometimes insufficient to classify the bacteria correctly. On that account, this study was undertaken to investigate the feasibility of three individual analytical techniques, capillary zone electrophoresis (CZE), capillary isoelectric focusing (CIEF), and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), for reliable classification of Dickeya and Pectobacterium species. Forty-three strains, representing different Dickeya and Pectobacterium species, namely Dickeya dianthicola, Dickeya dadantii, Dickeya dieffenbachiae, Dickeya chrysanthemi, Dickeya zeae, Dickeya paradisiaca, Dickeya solani, Pectobacterium carotovorum, and Pectobacterium atrosepticum, were selected for this purpose. Furthermore, the selected bacteria included one strain which could not be classified using traditional microbiological methods. Characterization of the bacteria was based on different pI values (CIEF), migration velocities (CZE), or specific mass fingerprints (MALDI-TOF MS) of intact cells. All the examined strains, including the undetermined bacterium, were characterized and classified correctly into respective species. MALDI-TOF MS provided the most reliable results in this respect.

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Acknowledgments

This work was supported by a grant from the Ministry of the Interior of the Czech Republic (No. VG20112015021) and with the institutional support (RVO: 68081715).

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Authors and Affiliations

  1. Institute of Analytical Chemistry of the ASCR, v. v. i., Veveří 97, 602 00, Brno, Czech Republic

    Jiří Šalplachta, Anna Kubesová, Marie Tesařová & Marie Horká

  2. Division of Diagnostics, Central Institute for Supervising and Testing in Agriculture, Šlechtitelů 23, 77900, Olomouc, Czech Republic

    Jaroslav Horký & Hana Matoušková

Authors
  1. Jiří Šalplachta
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  2. Anna Kubesová
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  6. Marie Horká
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Correspondence to Jiří Šalplachta.

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Šalplachta, J., Kubesová, A., Horký, J. et al. Characterization of Dickeya and Pectobacterium species by capillary electrophoretic techniques and MALDI-TOF MS. Anal Bioanal Chem 407, 7625–7635 (2015). https://doi.org/10.1007/s00216-015-8920-y

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  • DOI: https://doi.org/10.1007/s00216-015-8920-y

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