Abstract
In this contribution, the great potential of surface enhanced Raman spectroscopy (SERS) in a lab-on-a-chip (LOC) device for the detection of analyte molecules in a complex environment is demonstrated. Using LOC-SERS, the enzyme activity of thiopurine S-methyltransferase (TPMT) is analysed and identified in lysed red blood cells. The conversion of 6-mercaptopurine to 6-methylmercaptopurine catalysed by TPMT is observed as it gives evidence for the enzyme activity. Being able to determine the TPMT activity before starting a treatment using 6-mercaptopurine, an optimized dosage can be applied to each patient and serious toxicity appearing within thiopurine treatment will be prevented.
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Acknowledgement
We gratefully acknowledge the Free State of Thuringia and the European Union (EFRE) for financial support under support code 2008FE9112 and 2008FE9113 (BioOptiSens).
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Published in the special issue Biophotonics with Guest Editors Jürgen Popp and Reiner Salzer.
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März, A., Mönch, B., Rösch, P. et al. Detection of thiopurine methyltransferase activity in lysed red blood cells by means of lab-on-a-chip surface enhanced Raman spectroscopy (LOC-SERS). Anal Bioanal Chem 400, 2755–2761 (2011). https://doi.org/10.1007/s00216-011-4811-z
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DOI: https://doi.org/10.1007/s00216-011-4811-z