Skip to main content
SpringerLink
Log in

Development of a colloidal gold-based lateral-flow immunoassay for the rapid simultaneous detection of zearalenone and deoxynivalenol

  • Short Communication
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

A multianalyte lateral-flow technique using colloidal gold-labeled monoclonal antibodies was developed for the rapid simultaneous detection of deoxynivalenol (DON) and zearalenone (ZEA). The results of this qualitative one-step test were interpreted visually. A very simple and fast sample preparation was used, and the assay procedure could be accomplished within 10 min. When applied to spiked wheat samples, the technique gave accurate and reproducible results. Cut-off levels of 1500 and 100 μg kg−1 for DON and ZEA, respectively, were observed. The described multianalyte format can be used as a reliable, rapid and cost-effective on-site screening technique for the simultaneous determination of mycotoxins in grain samples.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  1. Cahill LM, Kruger SC, McAlice BT, Ramsey CS, Prioli R, Kohn B (1999) J Chromatogr A 859:23

    Article  CAS  Google Scholar 

  2. Krska R, Josephs R (2001) Fresenius J Anal Chem 369:469

    Article  CAS  Google Scholar 

  3. Langseth W, Rundberget T (1998) J Chromatogr A 815:103

    Article  CAS  Google Scholar 

  4. Vrabcheva T, Gessler R, Usleber E, Märtlbauer E (1996) Mycopathologia 136:47

    Article  CAS  Google Scholar 

  5. Curtui VG, Usleber E, Dietrich R, Lepschy J, Märtlbauer E (1998) Mycopathologia 143:97

    Article  CAS  Google Scholar 

  6. Curtui VG, Gareis M, Usleber E, Märtlbauer E (2001) Food Addit Contam 18:730

    Article  CAS  Google Scholar 

  7. Schollenberger M, Müller H-M, Rüfle M, Suchy S, Planck S, Drochner W (2005) Int J Food Microbiol 97:317

    Article  CAS  Google Scholar 

  8. Papadopoulou-Bouraoui A, Vrabcheva T, Valzacchi S, Stroka J, Anklam E (2004) Food Addit Contam 21:607

    Article  CAS  Google Scholar 

  9. Miller JD, Trenholm HL (eds) (1994) Mycotoxins in grain: compounds other than aflatoxins. Eagan, St. Paul, MN, p 359

  10. Stecher G, Jarukamjorn K, Zaborski P, Bakry R, Hucka CW, Bonn GK (2007) Talanta DOI 10.1016/j.talanta.2007.03.028

  11. Pichler H, Krska R, Székács A, Grasserbauer M (1998) Fresenius J Anal Chem 362:176

    Article  CAS  Google Scholar 

  12. Speijers GJA, Speijers MHM (2004) Toxicol Lett 153:91

    Article  CAS  Google Scholar 

  13. EC (2006) Commission Regulation 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. Off J Eur Union L364:5

    Google Scholar 

  14. Krska R, Baumgartner S, Josephs R (2001) Fresenius J Anal Chem 371:285

    Article  CAS  Google Scholar 

  15. Koch P (2004) Toxicol Lett 153:109

    Article  CAS  Google Scholar 

  16. Krska R, Welzig E, Berthiller F, Molinelli A, Mizaikoff B (2005) Food Addit Contam 22:345

    Article  CAS  Google Scholar 

  17. Schneider E, Curtui V, Seidler C, Dietrich R, Usleber E, Märtlbauer E (2004) Toxicol Lett 153:113

    Article  CAS  Google Scholar 

  18. Maragos C, Busman M, Sugita-Konishi Y (2006) Food Addit Contam 23:816

    Article  CAS  Google Scholar 

  19. Székács A (1998) Food Technol Biotechnol 36:105

    Google Scholar 

  20. Suzuki T, Munakata Y, Morita K, Shinoda T, Ueda H (2007) Anal Sci 23:65

    Article  Google Scholar 

  21. Yu W, Yu F-Y, Undersander DJ, Chu FS (1999) Food Agric Immunol 11:307

    Article  CAS  Google Scholar 

  22. Maragos CM, Jolley ME, Nasir MS (2002) Food Addit Contam 19:400

    Article  CAS  Google Scholar 

  23. Maragos CM, Plattner RD (2002) J Agric Food Chem 50:1827

    Article  CAS  Google Scholar 

  24. Nasir MS, Jolley ME (2003) Com Chem High T Scr 6(3):267

    Google Scholar 

  25. Lippolis V, Pascale M, Visconti A (2006) J Food Prot 69:2712

    CAS  Google Scholar 

  26. Maragos CM, Kim EK (2004) J Food Prot 67:1039

    CAS  Google Scholar 

  27. Schnerr H, Vogel RF, Niessen L (2002) Food Agric Immunol 14:313

    Article  CAS  Google Scholar 

  28. Tüdös AJ, Lucas-van den Bos ER, Stigter ECA (2003) J Agric Food Chem 51:5843

    Article  CAS  Google Scholar 

  29. Ngundi MM, Qadri SA, Wallace EV, Moore MH, Lassman ME, Shriver-Lake LC, Ligler FS, Taitt CR (2006) Environ Sci Technol 40:2352

    Article  CAS  Google Scholar 

  30. Ngundi MM, Shriver-Lake LC, Moore MH, Ligler FS, Taitt CR (2006) J Food Prot 69:3047

    CAS  Google Scholar 

  31. Carter RM, Blake RC, Mayer HP, Echevarria AA, Nguyen TD, Bostanian LA (2000) Anal Lett 33:405

    Article  CAS  Google Scholar 

  32. Berthiller F, Schuhmacher R, Buttinger G, Krska R (2005) J Chromatogr A 1062:209

    Article  CAS  Google Scholar 

  33. Sulyok M, Berthiller F, Krska R, Schuhmacher R (2006) Rapid Commun Mass Spectrom 20:2649

    Article  CAS  Google Scholar 

  34. Van der Gaag B, Spath S, Dietrich H, Stigter E, Boonzaaijer G, Van Osenbruggen T, Koopal K (2003) Food Control 14:251

    Article  CAS  Google Scholar 

  35. Abouzied MM, Pestka JJ (1994) J AOAC Int 77:495

    CAS  Google Scholar 

  36. Schneider E, Usleber E, Märtlbauer E, Dietrich R, Terplan G (1995) Food Addit Contam 12:387

    CAS  Google Scholar 

  37. Goryacheva IYu, De Saeger S, Delmulle B, Lobeau M, Eremin SA, Barna-Vetró I, Van Peteghem C (2007) Anal Chim Acta 590:118

    Article  CAS  Google Scholar 

  38. Saha D, Acharya D, Roy D, Shrestha D, Dhar TK (2007) Anal Chim Acta 584:343

    Article  CAS  Google Scholar 

  39. Gabaldon JA, Cascales JM, Morias S, Maquieira A, Puchades R (2003) Food Addit Contam 20:707

    Article  CAS  Google Scholar 

  40. Delmulle BS, De Saeger SMDG, Sibanda L, Barna-Vetró I, Van Peteghem C (2005) J Agric Food Chem 53:3364

    Article  CAS  Google Scholar 

  41. Wang S, Quan Y, Lee N, Kennedy IR (2006) J Agric Food Chem 54:2491

    Article  CAS  Google Scholar 

  42. Kolosova AYu, Shim W-B, Yang Z-Y, Eremin SA, Chung D-H (2006) Anal Bioanal Chem 384:286

    Article  CAS  Google Scholar 

Download references

Acknowledgement

This work was financially supported by IWT-Flanders (research project 020448). The authors would like to thank Dr. Chris Maragos (USDA) for providing DON monoclonal antibodies.

Author information

Authors and Affiliations

  1. Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000, Ghent, Belgium

    Anna Yu Kolosova, Sarah De Saeger & Carlos Van Peteghem

  2. TOXI-TEST NV, Industrielaan 9a, 9990, Maldegem, Belgium

    Liberty Sibanda

  3. Euro-Diagnostica BV, Beijerinckweg 18, 6827 BN, Arnhem, The Netherlands

    Ron Verheijen

Authors
  1. Anna Yu Kolosova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sarah De Saeger
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Liberty Sibanda
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ron Verheijen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Carlos Van Peteghem
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Anna Yu Kolosova.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kolosova, A.Y., De Saeger, S., Sibanda, L. et al. Development of a colloidal gold-based lateral-flow immunoassay for the rapid simultaneous detection of zearalenone and deoxynivalenol. Anal Bioanal Chem 389, 2103–2107 (2007). https://doi.org/10.1007/s00216-007-1642-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-007-1642-z

Keywords

Search

Navigation