Abstract
Microfluidic systems enable superior control of fluidics. We have developed a novel size-separation method utilizing secondary flow within a microchannel. Using confocal fluorescence microscopy and computer simulation, we confirmed that separation occurred as a result of specific molecular localization in the curving part of the microchannel. Maximum separation efficiency was achieved by optimizing microchannel design and flow rate for individual separation targets. In addition, more effective separation was achieved by use of plural microchannel curves. This method was used for sequence-selective DNA sensing. Double-stranded DNA formed by hybridization between target DNA and a complementary probe had different elution profiles from those of the single-stranded non-complementary sequence. Moreover, the response depends on the length of the DNA molecules. This method does not require immobilization of either probe or target DNA, because all reactions occurred in the solution phase. Such features may reduce experimental error and the difference between data from different operators.
Similar content being viewed by others
References
Larive CK, Lunte SM, Zhong M, Perkins MD, Wilson GS, Gokulrangan G, Williams T, Afroz F, Schöneich C, Derrick TS, Middaugh CR, Bogdanowich-Knipp S (1999) Anal Chem 71:389R–423R
Wang H, Hanash SJ (2003) Chromatogr B 787:11–18
Veenstra TD (1999) Biophys Chem 79:63–79
Wan KX, Shibue T, Gross ML (2000) J Am Chem Soc 122:300–307
Wang J (1999) Chem Eur J 5:1681–1685
Chan X, Zehnbauer B, Gnirke A, Kwok PY (1997) Proc Natl Acad Sci USA 94:10756–10761
Dubiley S, Kirilov E, Lysov Y, Mizabekov A (1997) Nucleic Acids Res 25:2259–2265
Pease AC, Solas D, Sullivan EJ, Cronin MT, Holmes CP, Fodor APA (1994) Proc Natl Acad Sci USA 91:5022–5026
Howell WM, Jobs M, Gyllensten U, Brookes AJ (1999) Nat Biotechnol 17:87–88
Hashimoto K, Ito K, Ishimori Y (1994) Anal Chem 66:3830–3833
Boon EM, Ceres DM, Drummond TG, Hill MG, Barton JK (2000) Nat Biotechnol 18:1096–1100
Takenaka S, Yamashita K, Takagi M, Uto Y, Kondo H (2000) Anal Chem 72:1334–1341
Marrazza G, Chiti G, Maschini M, Anichini M (2000) Clin Chem 46:31–37
Yamashita K, Yamaguchi Y, Miyazaki M, Nakamura H, Shimizu H, Maeda H (2004) Chem Eng J 101:157–161
Kenis PJA, Ismagilov RF, Whitesides GM (1999) Science 285:83–85
Weigl BH, Yager P (1999) Science 283:346–347
Tokeshi M, Minagawa T, Uchiyama K, Hibara A, Sato K, Hisamoto H, Kitamori T (2002) Anal Chem 74:1565–1571
Yamashita K, Yamaguchi Y, Miyazaki M, Nakamura H, Shimizu H, Maeda H (2004) Lab Chip 4:1–3
Kawazumi H, Tashiro A, Ogino K, Maeda H (2002) Lab Chip 1:8–10
Marin MC, Jost CA, Brooks LA, Irwin MS, O’Nions J, Tidy JA, James N, McGregor JM, Harwood CA, Yulug IG, Vousden KH, Allday MJ, Gusterson B, Ikawa S, Hinds PW, Crook T, Kaelin WG Jr (2000) Nat Genet 25:47–54
Kovacs GTA (1998) Micromachined transducer sourcebook. McGraw–Hill, Boston
Ismagilov RF, Stroock AD, Kenis PAJ, Whitesides GM (2000) Appl Phys Lett 76:2376–2378
Tanford C (1961) Physical chemistry of macromolecules. Wiley, New York
Atkins PW (1990) Physical chemistry, 4th edn. Oxford University Press, Oxford
Yamaguchi Y, Takagi F, Yamashita K, Maeda H (2004) AIChE J 50:1530–1535
Acknowledgements
We thank Professor Makoto Takagi of Kyushu University for helpful discussion. This study was supported by Industrial Technology Research Grant Program from NEDO. This work was also supported in part by grants from MEXT of Japan.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yamashita, K., Ogura, D., Yamaguchi, Y. et al. Specific molecule localization in microchannel laminar flow and its application for non-immobilized-probe analysis. Anal Bioanal Chem 382, 1477–1483 (2005). https://doi.org/10.1007/s00216-005-3368-0
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00216-005-3368-0