Skip to main content
SpringerLink
Log in

Molecularly-imprinted polypyrrole-modified stainless steel frits for selective solid phase preconcentration of ochratoxin A

  • Original Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

A molecularly-imprinted polymer (MIP) was prepared by electropolymerization of pyrrole (Py) onto a stainless steel frit, using ochratoxin A (OTA) as the template, in order to make a micro solid phase preconcentration (μSPP) device. The OTA template was removed with 1% triethylamine (TEA) in methanol. Compared to non-imprinted polypyrrole (PPy), the molecularly-imprinted polypyrrole (MIPPy) enhanced the selective binding of OTA. The percentage recovery improved from 0 to 40% when the OTA sample solution was acidified with 1 M HCl (1% by volume). At a flow rate of 0.2 mL/min, maximum OTA binding was reached in 6 min after a total loading of 3.2 ng OTA. Final elution of the OTA was analyzed by high performance liquid chromatography (HPLC) with fluorescence detection, using 20:80 v/v acetonitrile–ammonia buffer (NH4Cl/NH3, 20 mM, pH 9.2) as the mobile phase. The MIPPy-μSPP-HPLC results clearly demonstrated that the MIPPy-μSPP device afforded selective preconcentration of OTA from red wine samples, at OTA concentration levels as low as 0.05 ppb, prior to HPLC analysis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2a–b
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Monaci L, Palmisano F (2004) Anal Bioanal Chem 378:96–103

    Article  CAS  PubMed  Google Scholar 

  2. Valenta H (1998) J Chromatogr A 815:75–92

    Article  CAS  PubMed  Google Scholar 

  3. Domijan AM, Peraica M, Miletic-Medved M, Lucic A, Fuchs R (2003) J Chromatogr B 798:317–321

    Article  CAS  Google Scholar 

  4. Maier NM, Buttinger G, Welhartizki S, Gavioli D, Lindner W (2004) J Chromatogr A 804:103

    Article  CAS  Google Scholar 

  5. Jornet D, Busto O, Guasch J (2000) J Chromatogr A 882:29–35

    Article  CAS  PubMed  Google Scholar 

  6. Kataoka H (2003) Trends Anal Chem 22:232–244

    Article  CAS  Google Scholar 

  7. Alltech Chromatography Sourcebook (2004) Catalog 550:572–573

    Google Scholar 

  8. Li KM, Thompson MR, McGregor IS (2004) J Chromatogr B 804:319–326

    Article  CAS  Google Scholar 

  9. Wu J, Yu X, Lord H, Pawliszyn J (2000) Analyst 125:391–394

    Article  CAS  Google Scholar 

  10. Sadki S, Schottland P, Brodie N, Sabouraud G (2000) Chem Soc Rev 29:283–293

    Article  Google Scholar 

  11. Chen F, Shi G, Fu M, Qu L, Hong X (2003) Synth Met 132:125–132

    Article  CAS  Google Scholar 

  12. Wu J, Pawliszyn J (2001) J Chromatogr A 909:37–52

    Article  CAS  PubMed  Google Scholar 

  13. Liljegren G, Pettersson J, Markides KE, Nyholm L (2002) Analyst 127:591–597

    Article  CAS  PubMed  Google Scholar 

  14. Yu JCC, Lai EPC (2005) Anal Bioanal Chem 381:948–952

    Article  CAS  PubMed  Google Scholar 

  15. Korri-Youssoufi H, Garnier F, Kassmi AE, Godillot P, Srivastava P (1997) Synth Met 84:169–170

    Article  CAS  Google Scholar 

  16. Zhou SN, Lai EP, Miller JD (2004) Anal Bioanal Chem 378(8):1903–1906

    Article  CAS  PubMed  Google Scholar 

  17. Jodlbauer J, Maier NM, Lindner W (2002) J Chromatogr A 945:45–63

    Article  CAS  PubMed  Google Scholar 

  18. Ersoz A, Gavalas VG, Bachas LG (2002) Anal Bioanal Chem 372(7–8):786–790

    Article  PubMed  Google Scholar 

  19. Shiigi H, Kishimoto M, Yakabe H, Deore B, Nagaoka T (2002) Anal Sci 18(1):41–44

    Article  CAS  PubMed  Google Scholar 

  20. Ramanaviciene A, Ramanavicius A (2004) Biosens Bioelectron 20(6):1076–1082

    Article  CAS  PubMed  Google Scholar 

  21. Gong JL, Gong FC, Kuang Y, Zeng GM, Shen GL, Yu RQ (2004) Anal Bioanal Chem 379:302–307

    Article  CAS  PubMed  Google Scholar 

  22. Weetall HH, Rogers KR (2004) Talanta 62:329–335

    Article  CAS  Google Scholar 

  23. González-Peñas E, Leache C, Viscarret M, Pérez de Obanos A, Araguás C, López de Cerain A (2004) J Chromatogr A 1025:163–168

    Article  PubMed  Google Scholar 

  24. Blesa J, Soriano JM, Moltó JC, Mañes J (2004) J Chromatogr A 1054:397–401

    Article  CAS  PubMed  Google Scholar 

  25. Antonio M, Carmine N, Luca T, Pietro DL (2003) J Wine Res 14(2–3):115–120

    Article  Google Scholar 

  26. Visconti A, Pascale M, Centonze G (1999) J Chromatogr A 864:89–101

    Article  CAS  PubMed  Google Scholar 

  27. Stefanaki I, Foufa E, Tsatsou-Dritsa A, Dais P (2003) Food Addit Contam 20:74–83

    Article  CAS  PubMed  Google Scholar 

  28. Shephard GS, Fabiani A, Stockenström S, Mshicileli N, Sewram V (2003) J Agric Food Chem 51:1102–1106

    Article  CAS  PubMed  Google Scholar 

  29. Soufleros EH, Tricard C, Bouloumpasi EC (2003) J Sci Food Agric 83:173–179

    Article  CAS  Google Scholar 

  30. Yu JCC, Krushkova S, Lai EPC, Dabek-Zlotorzynska E (2005) Synth Met (submitted)

  31. Jodlbauer J, Maier NM, Lindner W (2002) J Chromatogr A 945:45–63

    Article  CAS  PubMed  Google Scholar 

  32. Maier NM, Buttinger G, Welhartizki S, Gavioli E, Lindner W (2004) J Chromatogr B 804:103–111

    Article  CAS  Google Scholar 

  33. Turner NW, Piletska EV, Karim K, Whitcombe M, Malecha M, Magan N, Baggiani C, Piletsky SA (2004) Biosens Bioelectr 20:1060–1067

    Article  CAS  Google Scholar 

  34. Feng SY, Lai EPC, Dabek-Zlotorzynska E, Sadeghi S (2004) J Chromatogr A 1027:155–160

    CAS  PubMed  Google Scholar 

Download references

Acknowledgments

Financial support of the Natural Sciences and Engineering Research Council (NSERC) Canada is gratefully acknowledged. S.K. thanks Environment Canada for funding of a Science Horizons Youth Internship Program placement.

Author information

Authors and Affiliations

  1. Department of Chemistry, Ottawa-Carleton Chemistry Institute, Carleton University, Ottawa, ON K1S 5B6, Canada

    Jorn C. C. Yu, Svetla Krushkova & Edward P. C. Lai

  2. Environmental Technology Centre, Environment Canada, 335 River Road, Ottawa, ON, K1A 0H3, Canada

    Ewa Dabek-Zlotorzynska

Authors
  1. Jorn C. C. Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Svetla Krushkova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Edward P. C. Lai
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ewa Dabek-Zlotorzynska
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Edward P. C. Lai.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yu, J.C.C., Krushkova, S., Lai, E.P.C. et al. Molecularly-imprinted polypyrrole-modified stainless steel frits for selective solid phase preconcentration of ochratoxin A. Anal Bioanal Chem 382, 1534–1540 (2005). https://doi.org/10.1007/s00216-005-3342-x

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-005-3342-x

Keywords

Search

Navigation