Abstract
The present research work was designed to examine the neuroprotective effect of ethanolic extract of Solanum virginianum Linn. (SV) in chronic construction injury (CCI) of sciatic nerve–induced neuropathic pain in rats. The extract was initially standardized by high-performance thin-layer chromatography using solasodine as a biomarker and was then subjected to assess the degree of mechanical allodynia, thermal allodynia, mechanical hyperalgesia, thermal hyperalgesia and biochemical evaluations. Administration of SV (100 and 200 mg/kg; p.o.) and pregabalin (10 mg/kg; p.o.) as a reference standard significantly debilitated hyperalgesia and allodynia and notably restored the altered antioxidant level and pro-inflammatory cytokine (IL-1β and TNF-α) expression in a dose-dependent manner. Further, to appraise the mechanistic approach of solasodine, docking simulation studies were done on the 3D structure of the voltage-gated N-type calcium channel (Cav 2.2), R-type calcium channel (Cav 2.3) and sodium channel (Nav 1.7), and the results revealed that solasodine properly positioned into Phe 19, Leu 32, Met 51 and Met 71 (FLMM pocket) of Cav 2.2 and Cav 2.3 and being a competitor of Ca2+/N-lobe it may inactivate these calcium channels but did not bind into the desired binding pocket of Nav 1.7. Thus, the study confirmed the role of solasodine as a major biomarker for the observed neuroprotective nature of Solanum virginianum.
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Acknowledgements
The authors would like to acknowledge the valuable contribution of the University Institute of Pharmaceutical Sciences, UGC Centre of Advanced Study, Panjab University, Chandigarh 160 014, India, for providing the lab facility for conducting the experimental work.
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SKS, AK and SKP designed the framework of the study, while RSP and SKP performed the phytochemical evaluations and standardization of extracts. SV, AK and RB were involved in the initial behavioural evaluation of the pharmacological studies. SV, AS and RSP were involved in performing the biochemical evaluations and cytokine estimations, while SKS and SKP performed the molecular docking studies and its interpretation. SV, AS, RB and RSP contributed significantly in compilation of results and interpretation of data. SV, SKS, SKP and AK contributed in initial drafting of the manuscript, which was further finalized by SKS and SKP. All the authors have read and approved the manuscript before its submission.
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The experimental protocol was approved by Institutional Animal Ethics Committee of the university (LRIP/IAEC/2019/PH-03) and was carried out in accordance with the guidelines of Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA), Government of India, and Indian National Science Academy Guidelines for the use and care of experimental animals (Publication No. 85–23, revised 1985)
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Verma, S., Kuhad, A., Bhandari, R. et al. Effect of ethanolic extract of Solanum virginianum Linn. on neuropathic pain using chronic constriction injury rat model and molecular docking studies. Naunyn-Schmiedeberg's Arch Pharmacol 393, 1715–1728 (2020). https://doi.org/10.1007/s00210-020-01872-8
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DOI: https://doi.org/10.1007/s00210-020-01872-8