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HP-β-CD-PLGA nanoparticles improve the penetration and bioavailability of puerarin and enhance the therapeutic effects on brain ischemia–reperfusion injury in rats

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Abstract

It is well known that puerarin attenuates ischemia–reperfusion injury and promotes function recovery of ischemic region. However, due to its reverse physiochemical properties, puerarin does not easily cross the blood–brain barrier. The aim of the present study is to create puerarin nanoparticles which increase and prolong the puerarin concentration in the brain. Using emulsion solvent evaporation techniques, we designed puerarin-loaded poly(d,l-lactic-co-glycolic acid) nanoparticles. Hydroxypropyl beta cyclodextrin (HP-β-CD) was used to increase the solubility of puerarin and gelatin to enhance viscosity of inner water phase, which improved puerarin entrapment. The drug release kinetics and nanoparticle degradation in phosphate buffered saline (PBS) were analyzed by electronic microscopy and high-performance liquid chromatography. Computerized tomography scans were used to detect the infarction volume and electroencephalogram (EEG) was recorded to estimate the recovery of brain function. The results showed that the combined HP-β-CD and gelatin significantly improved the entrapment efficiency. The infarction volume was significantly decreased on days 3 and 7 after the administration of puerarin nanoparticles compared with that of control and pure puerarin. EEG was also significantly improved. Puerarin nanoparticles are potentially applicable for the brain injury induced by ischemic–reperfusion.

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Acknowledgments

This work was supported by the Heilongjiang Provincial Natural Science Foundation (no. D201031), the Heilongjiang Provincial Postdoctoral Project (no. LRB08-313), and, in part, by a grant from the Innovation Fund of Daqing Hi-tech Industrial Development Zone (no. DQGX08YF035).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Neurosurgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China

    Hai-quan Tao, Hai-cheng Yang, Wei Ye, Li-zhuang Yang & Chuan-lu Jiang

  2. Department of Ophthalmology, First Affiliated Hospital of Harbin Medical University, Harbin, China

    Qingfeng Meng

  3. Department of Pharmaceutics, Daqing Branch, Harbin Medical University, Daqing, China

    Ming-hui Li, Hui Yu, Mei-fang Liu, Dan Du, Shou-li Sun, Yan-ming Wang, Da-ling Zhu & Hai-sheng Peng

  4. Research and Development of Natural Products Key Laboratory, Harbin Medical University, Daqing, China

    Ming-hui Li, Hui Yu, Mei-fang Liu, Dan Du, Shou-li Sun, Yan-ming Wang & Da-ling Zhu

Authors
  1. Hai-quan Tao
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  2. Qingfeng Meng
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  3. Ming-hui Li
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  4. Hui Yu
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  5. Mei-fang Liu
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  6. Dan Du
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  7. Shou-li Sun
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  8. Hai-cheng Yang
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  9. Yan-ming Wang
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  10. Wei Ye
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  11. Li-zhuang Yang
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  12. Da-ling Zhu
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  13. Chuan-lu Jiang
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  14. Hai-sheng Peng
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Corresponding authors

Correspondence to Chuan-lu Jiang or Hai-sheng Peng.

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The first two authors contributed equally to this work.

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Tao, Hq., Meng, Q., Li, Mh. et al. HP-β-CD-PLGA nanoparticles improve the penetration and bioavailability of puerarin and enhance the therapeutic effects on brain ischemia–reperfusion injury in rats. Naunyn-Schmiedeberg's Arch Pharmacol 386, 61–70 (2013). https://doi.org/10.1007/s00210-012-0804-5

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  • DOI: https://doi.org/10.1007/s00210-012-0804-5

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