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Archives of Toxicology

, Volume 92, Issue 4, pp 1581–1591 | Cite as

Effects of bisphenol A on ovarian follicular development and female germline stem cells

  • Xiaoqin Zhu
  • Geng G. Tian
  • Baoli Yu
  • Yanzhou Yang
  • Ji Wu
Reproductive Toxicology

Abstract

Bisphenol A (BPA), one of the most frequently detected emerging pollutants in the environment, has been implicated in adverse effects in male and female reproduction at extremely low concentrations. This study aimed to investigate the effects and potential mechanism of BPA on mouse ovarian follicular development and female germline stem cells (FGSCs). Female CD-1 adult mice were administered gradient concentrations of BPA (12.5, 25, and 50 mg/kg/day) by intraperitoneal injection. We found that the number of atretic ovarian follicles was significantly increased at high BPA concentrations. Additionally, the numbers of primordial follicles, primary follicles, and corpus luteum (CL) were significantly reduced at high BPA concentrations. Interestingly, the number of FGSCs was remarkably reduced in BPA-treated ovaries. Furthermore, the increased apoptotic rate of FGSCs in vitro was triggered by BPA accompanied by increased BPA concentrations. To investigate the mechanism of BPA in ovarian follicular development, 193 differentially expressed proteins were identified in BPA-treated ovaries by the isobaric tags for relative and absolute quantification-coupled 2D liquid chromatography-mass spectrometry technique. A total of 106 proteins were downregulated and 85 proteins were upregulated. Among these proteins, the apoptosis-related protein SAFB-like transcriptional modulator (SLTM) was remarkably upregulated, and this result was consistent with western blotting. Taken together, our results suggest that an ovarian follicular development, especially, the development of primordial follicles, primary follicles, and the CL, is inhibited by high BPA concentrations, and the ovarian follicle atresia is initiated by BPA through upregulated expression of SLTM. Furthermore, BPA induces apoptosis of cultured FGSCs. The effect of BPA on ovarian follicular development and FGSCs, especially the effect on FGSCs, suggests a novel mechanism of how BPA causes female infertility.

Keywords

Bisphenol A (BPA) Ovarian follicle development Female germline stem cells (FGSCs) Apoptosis SAFB-like transcriptional modulator (SLTM) Proteomics 

Abbreviations

BPA

Bisphenol A

FGSCs

Female germline stem cells

CL

Corpus luteum

DMSO

Dimethylsulfoxide

EDU

5-Ethynyl-2′-deoxyuridine

BSA

Bovine serum albumin

TUNEL

Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling

DAPI

4′,6-Diamidino-2-phenylindole

PI

Propidium iodide

SDS-PAGE

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

LC–MS/MS

Liquid chromatography–mass spectrometry

DEPs

Differentially expressed proteins

GO

Gene ontology

KEGG

Kyoto Encyclopedia of Genes and Genomes

Notes

Acknowledgements

This work was supported by National Natural Science Foundation of China (81720108017), National Basic Research Program of China (2017YFA0504201), and Shanghai Jiao Tong University Medicine-Engineering Fund (YG2017ZD11).

Supplementary material

204_2018_2167_MOESM1_ESM.doc (2.3 mb)
Supplementary material 1 (DOC 2318 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Xiaoqin Zhu
    • 1
  • Geng G. Tian
    • 1
  • Baoli Yu
    • 1
  • Yanzhou Yang
    • 2
  • Ji Wu
    • 1
    • 2
  1. 1.Renji Hospital, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, School of MedicineShanghai Jiao Tong UniversityShanghaiChina
  2. 2.Key Laboratory of Fertility Preservation and Maintenance of Ministry of EducationNingxia Medical UniversityYinchuanChina

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