Cyanotrophic and arsenic oxidizing activities of Pseudomonas mendocina P6115 isolated from mine tailings containing high cyanide concentration

  • Alejandra Miranda-Carrazco
  • Juan M. Vigueras-Cortés
  • Lourdes Villa-Tanaca
  • César Hernández-Rodríguez
Original Paper

Abstract

Mine tailings and wastewater generate man-made environments with several selective pressures, including the presence of heavy metals, arsenic and high cyanide concentrations, but severe nutritional limitations. Some oligotrophic and pioneer bacteria can colonise and grow in mine wastes containing a low concentration of organic matter and combined nitrogen sources. In this study, Pseudomonas mendocina P6115 was isolated from mine tailings in Durango, Mexico, and identified through a phylogenetic approach of 16S rRNA, gyrB, rpoB, and rpoD genes. Cell growth, cyanide consumption, and ammonia production kinetics in a medium with cyanide as sole nitrogen source showed that at the beginning, the strain grew assimilating cyanide, when cyanide was removed, ammonium was produced and accumulated in the culture medium. However, no clear stoichiometric relationship between both nitrogen sources was observed. Also, cyanide complexes were assimilated as nitrogen sources. Other phenotypic tasks that contribute to the strain’s adaptation to a mine tailing environment included siderophores production in media with moderate amounts of heavy metals, arsenite and arsenate tolerance, and the capacity of oxidizing arsenite. P. mendocina P6115 harbours cioA/cioB and aoxB genes encoding for a cyanide-insensitive oxidase and an arsenite oxidase, respectively. This is the first report where P. mendocina is described as a cyanotrophic and arsenic oxidizing species. Genotypic and phenotypic tasks of P. mendocina P6115 autochthonous from mine wastes are potentially relevant for biological treatment of residues contaminated with cyanide and arsenic.

Keywords

Cyanide Arsenic Mine wastes Pseudomonas 

Notes

Acknowledgements

We thank Ricardo Monterrubio-López and Luis Vázquez-Méndez for their support of determinations of metal concentration and electrical conductivity, respectively. We also thank First Majestic Plata SA de CV for the facilities for sampling. AMC thanks Consejo Nacional de Ciencia y Tecnologia (CONACYT) for the graduate scholarship awarded and Beca de Estimulo Institucional de Formación de Investigadores-Instituto Politécnico Nacional (IPN) for a scholarship complement. LVT, CHR, and JMVC are fellows of Estimulos al Desempeño de Investigadores-IPN, Comisión de Operación y Fomento de Actividades Académicas-IPN and Sistema Nacional de Investigadores-CONACYT. This work was supported by Secretaria de Investigación y Posgrado-IPN with Grants 20161850 and 20171849.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

203_2018_1514_MOESM1_ESM.pdf (183 kb)
Supplementary material 1 (PDF 182 KB)

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Authors and Affiliations

  1. 1.Departamento de Microbiología, Escuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalCd. MéxicoMexico
  2. 2.Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional-Unidad DurangoInstituto Politécnico NacionalDurangoMexico

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