Antibacterial potential of secondary metabolites produced by Aspergillus sp., an endophyte of Mitrephora wangii
Mitrephora wangii, an ethnomedicinal plant, has been used as a natural antibiotic and immunity booster in Thailand. A total of 22 fungi were isolated from M. wangii flowers. The fungal isolates were categorized into six genera including Agrocybe, Aspergillus, Colletotrichum, Nigrospora, Puccinia and Ustilago. Most extracts exhibited antibacterial activity against at least one of the test bacteria. Aspergillus sp. MFLUCC16-0845 was identified as the most bioactive fungus. Chemical composition of Aspergillus sp. MFLUCC16-0845 investigated using gas chromatography–mass spectrometry indicated that the major antibacterial compound was β-thujaplicin. Moreover, the newly isolated Aspergillus sp. MFLUCC16-0845 could be exploited as a potential source of bioactive compounds and plant defense activators. In addition, it is the first time that strain of Aspergillus sp. isolated and cultured from M. wangii flowers could produce β-thujaplicin at high yield with strong antimicrobial spectrum, which may lead to wide utilization in producing cosmetics and clinical products.
KeywordsAntibacterial Aspergillus Endophyte β-Thujaplicin Mitrephora wangii Monoterpene
The authors are thankful to Department of Medical Science, Ministry of Health, Bangkok, Thailand for bacterial pathogens. Authors express thanks to Institute of Excellence in Fungal Research and Botanical garden of Mae Fah Luang University for collecting of isolated fungal endophytes and identifying of plants, respectively. The financial support from Mae Fah Luang University is highly appreciated.
Compliance with ethical standards
Conflict of interest
The authors have no conflict of interest to declare.
- Adams RP (2007) Identification of essential oil components by gas chromatography/mass spectroscopy, 4th edn. Allured Publishing Corporation, IllinoisGoogle Scholar
- Arima Y, Nakai Y, Hayakawa R, Nishino T (2003) Antibacterial effect of β-thujaplicin on staphylococci isolated from atopic dermatitis: relationship between changes in the number of viable bacterial cells and clinical improvement in an eczematous lesion of atopic dermatitis. J Antimicrob Chemother 51:113–122CrossRefPubMedGoogle Scholar
- Barnett HL, Hunter BB (1998) Illustrated genera of imperfect fungi. Illustrated genera of imperfect fungi, 3rd edn. APS Press, MinnesotaGoogle Scholar
- Kurose D, Furuya N, Tsuchiya K, Tsushima S, Evans H (2012) Endophytic fungi associated with Fallopia japonica (Polygonaceae) in Japan and their interactions with Puccinia polygoni-amphibii var. tovariae, a candidate for classical biological control. Fungal Biol 116:785–791CrossRefPubMedGoogle Scholar
- Márquez SS, Bills GF, Zabalgogeazcoa I (2007) The endophytic mycobiota of the grass Dactylis glomerata. Fungal Divers 27:171–195Google Scholar
- Miller MA, Pfeiffer W, Schwartz T (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the gateway computing environments workshop (GCE), 14 November, Ieee, Louisiana, pp 1–8Google Scholar
- Stojanović-Radić Z, Čomić L, Radulović N, Blagojević P, Denić M, Miltojević A, Mihajilov-Krstev T (2012) Antistaphylococcal activity of Inula helenium L. root essential oil: eudesmane sesquiterpene lactones induce cell membrane damage. Eur J Clin Microbiol Infect Dis 31:1015–1025CrossRefPubMedGoogle Scholar
- Sureshjani MH, Yazdi FT, Mortazavi SA, Behbahani BA, Shahidi F (2014) Antimicrobial effects of Kelussia odoratissima extracts against food borne and food spoilage bacteria “in vitro”. J Paramed Sci 5:115–120Google Scholar
- Tanamatayarat P, Sotanaphun U, Limsirichaikul S, Girmay S (2012) Cytotoxic dihydrobenzofurans from Mitrephora Wangii Hu. Pharm Biol 50:578–579Google Scholar
- Weerasooriya AD, Saunders RM (2010) Systematic botany monographs: monograph of mitrephora (Annonaceae). American Society of Plant Taxonomists Press, MichiganGoogle Scholar
- Zhao X, Sugawara T, Kuroda S, Arisawa J, Kimura K (2009b) Antimicrobial activity of Beta-Thujaplicin (Hinokitiol) on heterotrophic bacteria isolated from reverse osmosis water using for the preparation of hemodialysis fluids. In: Proceedings of the world congress on medical physics and biomedical engineering, 7–12 September, Springer Berlin Heidelberg, Munich, pp 30–33Google Scholar