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Olivibacter flavus sp. nov., a novel endophytic bacterium isolated from the root of Camellia sinensis

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Abstract

A novel Gram-negative rod, endophytic bacterium, designated strain TMCC 8258T, was isolated from the root of Camellia sinensis collected from Puer, south-west China. Comparative 16S rRNA gene sequence analysis showed that the strain belongs to the family Sphingobacteriaceae and a neighbour-joining phylogenetic tree suggested that strain TMCC 8258T formed a cluster with the type strain of Olivibacter ginsengisoli (showed the highest 16S rRNA gene sequence similarity of 95.8%). Chemotaxonomic data [major fatty acid iso-C15:0, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), iso-C17:0 3-OH and major respiratory quinone MK-7] confirmed the affiliation of strain TMCC 8258T to the genus Olivibacter. The G + C content was 39.1 mol %. The results of the phylogenetic analysis, together with the physiological, morphological and biochemical tests, suggested that strain TMCC 8258T should be classified as representing a novel species of the genus Olivibacter, for which the name Olivibacter flavus is proposed. The type strain is TMCC 8258T (=CGMCC 1.16141 = KCTC 42683).

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (No. 31270055, 31160003, 31270055), Project for Key Laboratory of Food Biotechnology of Colleges and University in Sichuan (Project No. Szjj2013-045), Project of Office of Education of Sichuan Province, China (Project No. 13205688, 13ZB0024) and Project of the City of Chengdu (Project No. 2015-NY02-00007-NC).

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Author notes

  1. Lei Tian and Tong-Wei Guan contributed equally to this work.

Authors and Affiliations

  1. College of Resources, Sichuan Agricultural University, Chengdu, 611130, People’s Republic of China

    Lei Tian & Xiao-Ping Zhang

  2. Institute of Microbiology, Xihua University, Chengdu, 610039, People’s Republic of China

    Lei Tian & Tong-Wei Guan

  3. The Key Laboratory for Microbial Resources of Ministry of Education, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Lei Tian, Ling-Ling Yang, Kai-Yang Lu, Bing-Bing Liu, Wei-Xun ChunYu, Min Yin, En-Yuan Li, Yang Ji & Shu-Kun Tang

  4. Microbial Research and Development Center of TAETEA Group, Kunming, 650200, People’s Republic of China

    Kai-Yang Lu & Shu-Kun Tang

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  1. Lei Tian
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  2. Tong-Wei Guan
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Corresponding authors

Correspondence to Xiao-Ping Zhang or Shu-Kun Tang.

Additional information

Communicated by Erko Stackebrandt.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain TMCC 8258T is KT328577.

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Tian, L., Guan, TW., Yang, LL. et al. Olivibacter flavus sp. nov., a novel endophytic bacterium isolated from the root of Camellia sinensis . Arch Microbiol 199, 1237–1242 (2017). https://doi.org/10.1007/s00203-017-1391-0

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  • DOI: https://doi.org/10.1007/s00203-017-1391-0

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