Identification of Differentially Expressed Genes of Rice Under Cadmium Stress Using DDRT-PCR Approach

Abstract

Cadmium (Cd) is one of the hazardous environmental pollutants, and it can be harmful to human health through consumption of food-plants capable of bioaccumulating Cd. Therefore, lowering cadmium accumulation in plants is highly desirable. Here, a rice cultivar ‘Qisanzhan’ was studied using differential display reverse transcription-polymerase chain reaction (DDRT-PCR). Fifty-six differentially expressed genes were found in the root tips of 4-leaf stage rice seedlings exposed to 4 and 12 h of 50 µmol/L Cd(NO3)2 in a nutrient solution using DDRT-PCR. Further validation using semi-quantitative RT-PCR showed that the expression patterns of 16 genes were consistent with those found in DDRT-PCR. These genes encode receptor-like protein kinase, pleiotropic drug resistance protein, aquaporin protein, plasma membrane ATPase, etc. The differentially genes identified here can be used to obtain a better understanding of the molecular mechanisms of Cd absorption and accumulation in plants.

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Acknowledgements

We sincerely thank Dr. David Leung (University of Canterbury, New Zealand) for his critical reading and editing of this manuscript. This work was sponsored by the Research Team Project of the Natural Science Foundation of Guangdong Province (2016A030312009), NSFC-Guangdong Joint Fund (U1501233), the Science and Technology Program of Guangzhou, China (201707010032) and the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, China ([2007]1108).

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Correspondence to Ce-hui Mo or Xue-wen Hou.

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Wang, J., Zhao, H., Mo, C. et al. Identification of Differentially Expressed Genes of Rice Under Cadmium Stress Using DDRT-PCR Approach. Bull Environ Contam Toxicol 102, 589–594 (2019). https://doi.org/10.1007/s00128-019-02554-9

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Keywords

  • Cadmium
  • DDRT-PCR
  • Differential gene expression
  • Rice
  • sqRT-PCR