Abstract
Contamination of fresh water bodies like riverine system is utmost concerned environmental issue. This study was aimed to assess the concentration of heavy metals in River Kabul and their bioaccumulation by freshwater mussel. Comet assay was used to evaluate the genotoxic effect of heavy metals on hemocytes of freshwater mussel. The concentration of heavy metals in water was in the order of Zn > Pb > Ni > Cu > Mn > Fe > Cr > Cd, in sediments were Fe > Zn > Cr > Ni > Mn > Pb > Cu > Cd and in the soft tissues of freshwater mussel were in order of Fe > Zn > Mn > Pb > Cu > Cr > Ni > Cd. The hemocytes of mussels from polluted sites showed significantly higher (p < 0.05) DNA damage as compared to reference site. The study showed that pollutants from industries, municipal, domestic and agricultural sources cause heavy metals contamination in River Kabul.
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References
Akin HK, Unlu E (2007) Heavy metal concentrations in water, sediment, fish and some benthic organisms from Tigris River Turkey. Environ Monit Assess 131:323–337
Alloway BJ, Ayres DC (1993) Chemical principles of environmental pollution. Blackie, Academic. pp 140–149
Bryan GW, Langston WJ, Hummerstone LG (1980) The use of biological indicators of heavy metal contamination in estuaries. Occasional Publications Marine Biological Association of the United Kingdom occasional publications, Plymouth 1:1–73
Canli M, Ay O, Kalay M (1998) Levels of heavy metals (Cd, Pb, Cu, Cr and Ni) in tissues of Cyprinus carpio, Barbus capito and Chondrostoma regium from the Seyhan river Turkey. Turk J Zool 22:149–157
Chakraborty S, Ray M, Ray S (2010) Toxicity of sodium arsenite in the gill of an economically important mollusk of India. Fish Shellfish Immunol 29:136–146
Collins AR (2004) The comet assay for DNA damage and repair: principles, applications, and limitations. Mol Biotech 26:249–261
DWAF (Department of water affairs and forestry) (1996) South African water quality guidelines, vol 7 Aquatic ecosystem DWAF Pretoria
Farris JL, Van Hassel JH (2007) Freshwater bivalve ecotoxicology. CRC Press, New York, p 375
Fournier M, Pellerin J, Clermont Y, Morin Y, Brousseau P (2001) Effects of in vivo exposure of Myaarenaria to organic and inorganic mercury on phagocytic activity of haemocytes. Toxicol 161:210–211
Gundacker C (2000) Comparison of heavy metal bioaccumulation in freshwater mollusks of urban river habitats in Vienna. Environ Pollut 110:61–67
Hartwell LH, Hood L, Goldberg ML (2000) Genetics: from genes to genomes. McGraw Hill Higher Education, New York, pp 70–98
Igwilo IO, Afonne OJ, Maduabuchi UJ, Orisakwe OE (2006) Toxicological study of the Anambra River in Otuocha, Anambra State. Nigeria Arch Environ Occup Health 61:205–208
Jha AN, Dogra Y, Turner A, Millward GE (2005) Impact of low doses of tritium on the marine mussel, Mytilus edulis: genotoxic effects and tissue-specific bioconcentration. Mutat Res 586:47–57
Khan T, Muhammad S, Khan B, Khan H (2011) Investigating the levels of selected heavy metals in surface water of Shah Alam River (A tributary of River Kabul, Khyber Pakhtunkhwa). J Himalayan Earth Sci 44:71–79
Khisroon M, Khan A, Naseem M, Ali N, Khan S, Rasheed SB (2015a) Evaluation of DNA damage in lymphocytes of radiology personnel by comet assay. J Occup Health 57:268–274
Khisroon M, Gul A, Khan A, Ali N, Zaidi F, Rasheed SB, Ahmadullah (2015b) Comet assay based DNA evaluation of fuel filling stations and automobile workshop workers from Khyber Pakhtunkhwa province, Pakistan. J Occup Med Toxicol 10:27
Khisroon M, Khan A, Imran M, Zaidi F, Ahmadullah Fatima SH (2017) Biomonitoring of DNA damage in individuals exposed to brick kiln pollution from Peshawar, Khyber Pakhtunkhwa, Pakistan. Arch Environ Occup Health 73:115–120
Królak E, Zdanowski B (2001) The bioaccumulation of heavy metals by the mussels Anodonta woodiana (Lea, 1834) and Dreissena polymorpha (Pall.) in the heated Konin lakes. Arch Pol Fish 9:229–237
Liu H, Yang J, Gan J (2010) Trace element accumulation in bivalve mussels Anodonta woodiana from Taihu Lake. China Arch Environ Contam Toxicol 59:593–601
Malle KG (1992) Zink in der Umwelt. Acta Hydrochimica et Hydrobiologica 20:196–204
Matsumoto ST, Janaina R, Mario SM, Maria AM (2005) Evaluation of the genotoxic potential due to the action of an effluent contaminated with chromium, by the comet assay in CHO-K1 cultures. Caryologia 58:40–46
Morillo J, Usero J, Gracia I (2004) Heavy metal distribution in marine sediments from the southwest coast of. Spain Chemosphere 55:431–442
NEQS (National Environmental Quality Standards) (2010) The gazette of Pakistan, extraordinary published by authority Islamabad, Part II. Statutory notifications (S.R.O). Ministry of Environment Notifications Government of Pakistan
Oguzie FA, Okhagbuzo GA (2010) Concentrations of heavy metals in effluent discharges downstream of Ikpoba river in Benin City. Nigeria Afr J Biotechnol 9:319–325
Pandy S, Parvez S, Sayeed I, Haque R, Bin-Hafeez B, Raisuddin S (2003) Biomarkers of oxidative stress: a comparative study of river Yamuna fish Wallagoattu (Bl. & Schn). Sci Total Environ 309:105–115
Pavlica M, Klobucar GIV, Vetma N, Erben R, Papes D (2000) Detection of micronuclei in haemocytes of zebra mussel and great ramshorn snail exposed to pentachlorophenol. Mutat Res 465:145–150
Phillips DJH, Rainbow PS (1988) Barnacles and mussels as biomonitors of trace elements: a comparative study. Marine Ecol Prog Ser 49:83–93
Pourang N, Richardson CA, Mortazavi MS (2010) Heavy metal concentrations in the soft tissues of swan mussel (Anodonta cygnea) and surficial sediments from Anzali wetland, Iran. Environ Monit Asses 163:195–213
Ravera O, Beone GM, Cenci R, Lodigiani P (2003a) Metal concentrations in Unio pictorum mancus (Mollusca, Lamellibranchia) from of 12 Northern Italian lakes in relation to their trophic level. J Limnol 62:121–138
Ravera O, Cenci R, Beone GM, Dantas M, Lodigiani P (2003b) Trace element concentrations in freshwater mussels and macrophytes as related to those in their environment. J Limnol 62:61–70
Ravera O, Trincherini PR, Beone GM, Maiolini B (2005) The trend from 1934 to 2001 of metal concentrations in bivalve shells (Unio pictorum) from two small lakes: Lake Levico and Lake Caldonazzo (Trento Province, Northern Italy). J Limnol 64:113–118
Serpi U, Ayla C, Ozlem FC, Aysin G (2003) Antioxidant and pro-oxidant effects of tannins in digestive cells of the freshwater mussel Unio tumidus. Mutat Res 539:19–28
Serpi U, Ayla C, Ozlem FC, Aysin G (2006) Cadmium-induced genotoxicity, cytotoxicity and lipid peroxidation in Allium sativum and Viciafaba. Oxford J Life Sci Med Mutag 21:77–81
Siraj M, Khisroon M, Khan A (2016) Bioaccumulation of heavy metals in different organs of Wallago attu from River Kabul Khyber Pakhtunkhwa, Pakistan. Biol Trace Elem Res 12:242–250
Siraj M, Khisroon M, Khan A, Zaidi F, Ullah A, Rahman G (2018) Bio-monitoring of tissue accumulation and genotoxic effect of heavy metals in Cyprinus carpio from River Kabul Khyber Pakhtunkhwa Pakistan. Bull Environ Contam Toxicol 100:344–349
Tabinda AB, Bashir S, Yasar A, Hussain M (2013a) Metals concentrations in the riverine water, sediments and fishes from river Ravi at Balloki headworks. J Anim Plant Sci 23:76–84
Tabinda AB, Bashir S, Yasar A, Hussain M (2013b) (20013) Heavy Metals concentrations in water, sediments and fishes in river Sutlej at Sulemanki headworks. Pak J Zool 45:1663–1668
Tanabe S, Subramanian A (2006) Bioindicators of POPs: monitoring in developing countries. Kyoto University Press, Kyoto
Tekin-Ozan S, Aktan N (2012))mRelationship of heavy metals in water, sediment and tissues with total length, weight and seasons of Cyprinus carpio L., 1758 From Işikli Lake (Turkey). Pakistan J Zool 44:1405–1416
Usero J, Izquierdo C, Morillo J, Gracia I (2003) Heavy metals in fish (Solea vulgaris, Anguilla anguilla and Liza aurata) from Salt Marshes on the Southern Atlantic Coast of Spain. Environ Int 1069:1–8
Zhou XW, Zhu GN, Sun JH, Mwalilino J (2002) The liver cellular DNA breaks and repair of the fish (Carassius auratus) induced by the sublethal metal mixture. Nuclear Tech 25:408–412
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Khan, M.I., Zahoor, M., Khan, A. et al. Bioaccumulation of Heavy Metals and their Genotoxic Effect on Freshwater Mussel. Bull Environ Contam Toxicol 102, 52–58 (2019). https://doi.org/10.1007/s00128-018-2492-4
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DOI: https://doi.org/10.1007/s00128-018-2492-4