Histological and Genotoxic Biomarkers in Prochilodus lacustris (Pisces, Prochilodontidae) for Environmental Assessment in a Protected Area in the Northeast of Brazil

  • Rayssa de Lima CardosoEmail author
  • Raimunda Nonata Fortes Carvalho-Neta
  • Antonio Carlos Leal de Castro
  • Cássia Fernanda Chagas Ferreira
  • Marcelo Henrique Lopes Silva
  • James Werllen de Jesus Azevedo
  • João Reis Salgado Costa Sobrinho
  • Débora Martins Silva Santos


The quality of aquatic environments all around the world is being altered by different human activities that represent direct threat to the ecological system and the aquatic biota. This study aimed to evaluate the occurrence of histological and genotoxic alterations in Prochilodus lacustris as indicators of anthropic impacts in a lacustrine environment in northeast Brazil. The histological alterations were evaluated using the histological alteration index, and the genotoxic alterations were detected using the micronuclei test, at three sampling stations (S1, S2 and S3). The gills presented lesions with three stages of severity, with mild lesions more frequent in the specimens collected at station S1. Mild hepatic tissue lesions were the most frequent type in both areas. Micronucleus analysis showed that station S3 was the most affected. The biological responses observed indicated that the fish are under influence of environmental changes. It is important to highlight that the organisms collected at station S3 had a more compromised health status.


Liver Gill Histopathology Micronuclei Biomarkers 



The authors are grateful to the Biology and Aquatic Environment Study Group (BIOAqua/UEMA), the Animal Morphology Laboratory, the Aquatic Organism Biomarker Laboratory (LABOAQ/UEMA), the Fishing and Aquatic Ecology Laboratory (LABPEA/UEMA), the Soil Chemistry Laboratory (UEMA), the UEMA (Veterinary Medicine Department) Food and Water Microbiology Laboratory and the Oceanography Department of the Universidade Federal do Maranhão (DEOLI/UFMA) for assistance with the laboratory analyses.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Rayssa de Lima Cardoso
    • 1
    Email author
  • Raimunda Nonata Fortes Carvalho-Neta
    • 2
  • Antonio Carlos Leal de Castro
    • 3
  • Cássia Fernanda Chagas Ferreira
    • 2
  • Marcelo Henrique Lopes Silva
    • 4
  • James Werllen de Jesus Azevedo
    • 3
  • João Reis Salgado Costa Sobrinho
    • 5
  • Débora Martins Silva Santos
    • 2
  1. 1.Institute of Science and TechnologySão Paulo State University (Unesp)São PauloBrazil
  2. 2.Department of Chemistry and Biology, Postgraduate Program in Aquatic Resources and FisheriesState University of Maranhão (Uema)São LuísBrazil
  3. 3.Department of Oceanography and LimnologyFederal University of Maranhão (Ufma)São LuísBrazil
  4. 4.Postgraduate Program in Biodority and Biotechnology Network of the Legal Amazon (BIONORTE), Department of BiologyFederal University of Maranhão (Ufma)São LuísBrazil
  5. 5.Department of Agricultural Engineering, Soil Chemistry LaboratoryState University of Maranhão (Uema)São LuísBrazil

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