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Ecotoxicological Test Assay Using OECD TG 212 in Marine Java Medaka (Oryzias javanicus) and Freshwater Japanese Medaka (Oryzias latipes)

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Abstract

The lethal effects of chemicals is a serious concern to the protection of ecosystems, and the OECD TG 212 was established to estimate the lethal and sublethal effects on embryo and sac-fry stages of fish. It is still unclear, however, whether this test can effectively estimate the impacts of chemicals using marine fish. Therefore, this study aimed to use a recognized testing method on the marine fish Oryzias javanicus, and to assess differences in response to organotin compounds between a freshwater congener (Oryzias latipes) and O. javanicus. The lowest observed effect concentration (LOEC) of triphenyltin for lethal effect was the same in both species. The LOEC of tributyltin for lethal and sublethal effects were the same in both species. Our results provide the first evidence that O. javanicus and O. latipes are similarly affected by organotin compounds, suggesting that O. javanicus is a good model marine fish for the ecotoxicological assessment of chemicals.

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Acknowledgements

This study was supported partly by the Kurita Water and Environment Foundation, Japan (Grant No. 17B008) and a grant from the Ministry of Education, Culture, Sports, Science and Technology, Japan (Grant-in-Aid for Young Scientists (B) Grant No. 17K12828).

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Authors and Affiliations

  1. Faculty of Bioresource Sciences, Akita Prefectural University, 241-438 Kaidobata-Nishi, Nakano Shimoshinjo, Akita, 010-0195, Japan

    Yoshifumi Horie & Kei Yonekura

  2. Faculty of System Science and Technology, Akita Prefectural University, 84-4 Ebinokuchi, Tsuchiya, Yurihonjo, Akita, 015-0055, Japan

    Nobuhiro Kanazawa

  3. Center for Health and Environmental Risk Research, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki, 305-8506, Japan

    Takahiro Yamagishi

  4. Graduate School of Agriculture, Ehime University, Tarumi 3-5-7, Matsuyama, 790-8566, Japan

    Norihisa Tatarazako

Authors
  1. Yoshifumi Horie
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  2. Nobuhiro Kanazawa
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  3. Takahiro Yamagishi
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  4. Kei Yonekura
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  5. Norihisa Tatarazako
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Correspondence to Yoshifumi Horie.

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Horie, Y., Kanazawa, N., Yamagishi, T. et al. Ecotoxicological Test Assay Using OECD TG 212 in Marine Java Medaka (Oryzias javanicus) and Freshwater Japanese Medaka (Oryzias latipes). Bull Environ Contam Toxicol 101, 344–348 (2018). https://doi.org/10.1007/s00128-018-2398-1

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  • DOI: https://doi.org/10.1007/s00128-018-2398-1

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