Field Survey and Comparative Study of Pteris Vittata and Pityrogramma Calomelanos Grown on Arsenic Contaminated Lands with Different Soil pH

  • Bui Thi Kim Anh
  • Nguyen Ngoc Minh
  • Nguyen Thi Hoang Ha
  • Dang Dinh Kim
  • Nguyen Trung Kien
  • Nguyen Quang Trung
  • Tran Thien Cuong
  • Luu Thai Danh


In field survey, Pteris vittata and Pityrogramma calomelanos were only found in arsenic (As) contaminated areas with soil pH 7.2–8.8 and 2.3–4.2, respectively. In the first pot experiment, two fern species were grown on the soil amended with 300 mg kg−1 As at soil pH of 5.1, 7.2 and 9. P. calomelanos survived all pH treatments, and had the highest frond As concentration and soil As removal efficiency at soil pH 5.1. All P. vittata plants were dead at soil pH 5.1. P. vittata had higher frond As concentration, biomass and the amount of As removed from the soil than those of P. calomelanos at soil pH of 7.2 and 9. In the second pot experiment, P. vittata was demonstrated to have greater life time, biomass, As tolerance and accumulation than those of P. calomelanos as planted on alkaline soil (pH 7.8) spiked with various concentrations of As.


Pteris vittta Pityrogramma calomelanos Arsenic Soil pH Phytoextraction 



This research was funded by the National Foundation for Science and Technology Development of Vietnam (NAFOSTED) under Grant Number 105.08-2014.12.


  1. Anh BTK, Kim DD, Kuschk P, Tua TV, Hue NT, Minh NN (2013) Effect of soil pH on arsenic hyperaccumulation capacity in Pityrogramma calomelanos L. J Environ Biol 34:237–242Google Scholar
  2. Carbonell-Barrachina AA, Aarabi MA, Delaune RD, Gambrell RP, Patrick, WHJr (1998) The influence of arsenic chemical form and concentration on Spartina patens and Spartina alterniflora growth and tissue arsenic concentration. Plant Soil 198:33–43CrossRefGoogle Scholar
  3. Carrier M, Loppinet-Serani A, Absalon C, Marias F, Aymonier C, Mench M (2011) Conversion of fern (Pteris vittata L.) biomass from a phytoremediation trial in sub- and supercritical water conditions. Biomass Bioenerg 35:872–883CrossRefGoogle Scholar
  4. Cox MS, Bell PE (1996) Differential tolerance of canola to arsenic when grown hydroponically or in soil. J Plant Nutr 19:1599–1610CrossRefGoogle Scholar
  5. Cullen WR, Reimer KJ (1989) Arsenic speciation in the environment. Chem Rev 89:713–764CrossRefGoogle Scholar
  6. Cunningham SD, Shann JR, Crowley DE, Anderson TA (1997) Phytoremediation of contaminated water and soil. In: Kruger EL, Anderson TA, Coats JR (eds) Phytoremediation of soil and water contaminants. ACS symposium series 664. American Chemical Society, Washington (DC), pp 2–19CrossRefGoogle Scholar
  7. Danh LT, Truong P, Mammucari R, Foster N (2014) A critical review of the arsenic uptake mechanisms and phytoremediation potential of Pteris vittata. Int J Phytoremed 16:429–453CrossRefGoogle Scholar
  8. Duc L, Trinh NCT, Dzung PV, Nhan NTT (2008) Arsenic compounds in soil contaminated by mining activities in Thai Nguyen province. Vietnam Soil Sci 30:87–105Google Scholar
  9. Ha NT, Sakakibara M, Sano S, Nhuan MT (2011) Uptake of metals and metalloids by plants growing in a lead–zinc mine area, Northern Vietnam. J Hazard mater 186(2–3):1384–1391CrossRefGoogle Scholar
  10. Khan AG (2005) Role of soil microbes in the rhizospheres of plants growing on trace metal contaminated soils in phytoremediation. J Trace Elem Med Biol 18:355–364CrossRefGoogle Scholar
  11. Kukier U, Peters CA, Chaney RL, Angle JS, Roseberg RJ (2004) The effect of pH on metal accumulation in two species. J Environ Qual 33(6):2090–2102CrossRefGoogle Scholar
  12. Liao XY, Chen TB, Lei M, Huang ZC, Xiao XY, An ZZ (2004) Root distributions and elemental accumulations of Chinese brake (Pteris vittata L.) from As-contaminated soils. Plant Soil 261(1–2):109–116CrossRefGoogle Scholar
  13. Ma LQ, Komar KM, Tu C, Zhang W, Cai Y, Kennelley ED (2001) A fern that hyperaccumulates arsenic. Nature 409(6820):579–579CrossRefGoogle Scholar
  14. Niazi NK, Singh B, Van Zwieten L, Kachenko AG (2011) Phytoremediation potential of Pityrogramma calomelanos var. austroamericana and Pteris vittata L. grown at a highly variable arsenic contaminated site. Int J Phytorem 13(9):912–932CrossRefGoogle Scholar
  15. Niazi NK, Singh B, Van Zwieten L, Kachenko AG (2012) Phytoremediation of an arsenic-contaminated site using Pteris vittata L. and Pityrogramma calomelanos var. austroamericana: a long-term study. Environ Sci Pollut Res 19(8):3506–3515CrossRefGoogle Scholar
  16. Padmavathiamma PK, Li LY (2007) Phytoremediation technology: hyper-accumulation metals in plants. Water Air Soil Pollut 184:105–126CrossRefGoogle Scholar
  17. Ryan J, Estefan G, Rashid A (2007) Soil and plant analysis laboratory manual. ICARDA, AleppoGoogle Scholar
  18. Tu S, Ma LQ (2003) Interactive effects of pH, arsenic and phosphorus on uptake of As and P and growth of the arsenic hyperaccumulator Pteris vittata L. under hydroponic conditions. Environ Exp Bot 50(3):243–252CrossRefGoogle Scholar
  19. Tu S, Ma L, Luongo T (2004) Root exudates and arsenic accumulation in arsenic hyperaccumulating Pteris vittata and non-hyperaccumulating Nephrolepis exaltata. Plant Soil 258(1):9–19CrossRefGoogle Scholar
  20. Visoottiviseth P, Francesconi K, Sridokchan W (2002) The potential of Thai indigenous plant species for the phytoremediation of arsenic contaminated land. Environ Pollut 118:453–461CrossRefGoogle Scholar
  21. Yan XL, Chen TB, Liao XY, Huang ZC, Pan JR, Hu TD, Nie CJ, Xie H (2008) Arsenic transformation and volatilization during incineration of the hyperaccumulator Pteris vittata L. Environ Sci Technol 42:1479–1484CrossRefGoogle Scholar
  22. Yong JW, Tan SN, Ng YF, Low KK, Peh SF, Chua JC, Lim AA (2010) Arsenic hyperaccumulation by Pteris vittata and Pityrogramma calomelanos: a comparative study of uptake efficiency in arsenic-treated soils and waters. Water Sci Technol 61(12):3041–3049Google Scholar
  23. Zhao FJ, Dunham SJ, McGrath SP (2002) Arsenic hyperaccumulation by different fern species. New Phytol 156:27–31CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Vietnam Academy of Science and TechnologyHanoiVietnam
  2. 2.VNU University of ScienceHanoiVietnam
  3. 3.Can Tho UniversityCan ThoVietnam

Personalised recommendations