Effects of Dracontomelon duperreanum Leaf Litter on the Growth and Photosynthesis of Microcystis aeruginosa
This study investigated the use of Dracontomelon duperreanum leaf litter extract (DDLLE) in inhibiting the growth and photosynthesis of the algae Microcystis aeruginosa. The goal of the study was to evaluate a potential solution for cyanobacterial bloom prevention. M. aeruginosa was exposed to extract concentrations from 0.4 to 2.0 g L−1. Chlorophyll-a (Chl-a) content and photosynthesis levels were assessed using pulse amplitude modulated fluorimetry phytoplankton analyzer. Results suggested that the extract could efficiently suppress M. aeruginosa growth. The content of Chl-a was only 19.0 µg L−1 and achieved 96.0% inhibition rate when exposed to 2.0 g L−1 on day 15. Growth rate in response to different extract concentrations were consistent with changes in the photosynthesis efficiency (alpha), maximal relative electron transport rate and maximal photochemical efficiency of photosystem II (F v /F m ). Furthermore, several kinds of volatile chemicals and their concentrations in DDLLE had been identified by GC–MS, which of them play major role to suppress the growth of M. aeruginosa should be further studied.
KeywordsAllelopathy Dracontomelon duperreanum Growth suppression Microcystis aeruginosa Phyto-PAM
This work was supported by the National Natural Science Foundation (NSFC) of China, Grant No. 5137836 and the Science, Industry, Trade and Information Technology Commission of Shenzhen Municipality (JCYJ20150430163657307) for their financial support.
- Gao L, Xie L (2011) Analysis of the influence of meteorological condition on cyanobacterial bloom and treatment methods in Taihu Lake. China Resour Compr Utilization 29:35–38 (In Chinese)Google Scholar
- Jia CS (2006) Calculatiang the LC50 of insecticides with software SPSS. Chin Bull Entomol 43:414–417 (Chinese)Google Scholar
- Macías FA, Oliva RM, Simonet AM, Galindo JCG (1998) What are allelochemicals? In: Olofsdotter M (ed) Allelopathy in Rice. IRRI Press, ManillaGoogle Scholar
- Pan Q, Zou GY, Song XF, Nakai SS, Fu ZS (2014) Inhibitory effects of the roots of floating bed plants of Canna indica on Microcystis aeruginosa. Res of Environ Sci 27:193–1198 (Chinese)Google Scholar
- Platt T, Gallegos CL, Harrison WG (1980) Photoinhibition of photosynthesis in natural assemblages of marine phytoplankton. J Mar Res 38:687–701Google Scholar
- Rice EL (1984) Allelopathy, 2nd edn. Academic Press, OrlandoGoogle Scholar
- Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY (1979) Genetic assignments, stain histories and properties of pure culture of cyanobacteria. J Gen Microbiol 111:1–61Google Scholar
- Su XF, Nong WT (2010) Study on antimicrobial activity of extracts from Dracontomelon duperreanum. Chin Pharm 21:2115–2117 (Chinese)Google Scholar