Abstract
The widespread contamination and persistence of the herbicide butachlor in the environment resulted in the exposure of non-target organisms. The present study investigated the toxicity effect of butachlor (1–15 µmol/L) and the protective effect of vitamin C (VC) against butachlor-induced toxicity in zebrafish. It was found that butachlor significantly increased the mortality and malformation rates in a dose-dependent manner, which caused elevation in reactive oxygen species (ROS) and malondialdehyde (MDA) after 72 h exposure. Compared with butachlor treatment group, the protective effect of VC against butachlor-induced toxicity were observed after adding 40, 80 mg/L VC respectively. VC significantly decreased the mortality, malformation rates, ROS, MDA, and normalized antioxidant enzymes activities of zebrafish after 72 h exposure. The result shows VC has mitigative effect on butachlor-induced toxicity and it can be used as an effective antioxidant in aquaculture.
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Acknowledgements
This work was supported by grants from the Project of Science and Technology in Zhejiang Food and Drug Administration (SP201717) and the Key Innovation Team of Science and Technology in Zhejiang Province (2010R50018). All authors participated in writing and editing of this manuscript. There are no conflicts of interest to declare.
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Xiang, Q., Xu, B., Ding, Y. et al. Oxidative Stress Response Induced by Butachlor in Zebrafish Embryo/Larvae: The Protective Effect of Vitamin C. Bull Environ Contam Toxicol 100, 208–215 (2018). https://doi.org/10.1007/s00128-017-2245-9
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DOI: https://doi.org/10.1007/s00128-017-2245-9