Abstract
A green technique was designed for assessing toxic equivalence (TEQ) levels of polychlorinated dibenzo-p-dioxins/furans (PCDD/Fs) in sediments and soil from dioxin-contaminated areas. This technique combines a fast cleanup system with a dioxin-responsive-element (DRE)-driven luciferase bioassay. Samples from sediment (n = 10) and soil (n = 11) were analyzed with the technique; levels of PCDD/Fs ranged from 75.1 to 2,670 ng DRE-driven luciferase activity (DL)-TEQ/kg dry weight (d.w.). Significant correlations were found between DL-TEQs (by the bioassay) and PCDD/F WHO-TEQs (by HRGC/HRMS). DL-TEQs is significantly correlated with WHO-TEQs of 2,3,7,8-TCDD and 1,2,3,7,8-PeCDD using a multiple stepwise linear regression model (adjusted R2 = 0.962, p < 0.001).
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Acknowledgments
The research was supported by a grant (EPA-98-1604-02-01) from the Environmental Analysis Laboratory of Taiwan’s Environmental Protection Administration. The authors thank the researchers of EAL/EPA for assisting with analysis of PCDD/Fs in chemical assay. We are grateful to Mr. Mark Swofford for critical editing of the manuscript.
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How-Ran Chao and Ya-Fen Wang are the authors contributed equally.
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Chao, HR., Wang, YF., Lin, DY. et al. Fast Cleanup System Combined with a Dioxin-responsive Element-driven Luciferase Bioassay for Analysis of Polychlorinated Dibenzo-p-dioxins/furans in Sediments and Soils. Bull Environ Contam Toxicol 86, 278–282 (2011). https://doi.org/10.1007/s00128-011-0205-3
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DOI: https://doi.org/10.1007/s00128-011-0205-3