Fig. 6 | Diabetologia

Fig. 6

From: Mesenchymal stromal cell-derived exosomes ameliorate peripheral neuropathy in a mouse model of diabetes

Fig. 6

 MSC-exosomes reduce inflammatory response via regulating target gene expression. (a, b) MSC-exosome treatment reduced serum TNF-α (a) and IL-1β (b). (c) Representative images of western blot to assess levels of markers of M1 and M2 macrophages in sciatic nerves of mice treated with MSC-exosomes or saline in vivo. n = 8/group. (d, e) Quantification of relative expression levels of markers of M1 and M2 macrophages, respectively, in sciatic nerves from all groups. n = 5/group. (f, g) Representative images of western blot and the relative expression levels of M1 and M2 markers in intraperitoneal macrophages isolated from db/db mice that were treated with MSC-exosomes at a dose of 108/well, 109/well or PBS for 48 h in vitro. n = 3/group. Data are mean ± SEM and were analysed by one-way ANOVA with Tukey’s post hoc test. ††p < 0.01, †††p < 0.001 db/db vs db/m; *p < 0.05, **p < 0.01 db/db vs db + exo. db/db, diabetic group treated with saline (or PBS in f, g); db + exo, diabetic group treated with MSC-exosomes; db/m, non-diabetic group

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