Fig. 4 | Diabetologia

Fig. 4

From: Mesenchymal stromal cell-derived exosomes ameliorate peripheral neuropathy in a mouse model of diabetes

Fig. 4

 Protective effect of MSC-exosomes on the myelinated axon fibres in the sciatic nerve of diabetic mice. (a) Semi-thin toluidine blue-stained cross-sections of sciatic nerves; scale bar, 20 μm. (b) Scatter plot shows g-ratios of individual fibres as a function of the respective axon diameter. MSC-exosomes reduced the hypomyelination state of diabetic mice across all axon diameters but predominantly of the small and medium diameter axons. (c, d) Histograms showing the frequency distribution of the diameters of axons (c) and myelinated fibres (d). n = 8 per group; >300 axons per animal. Representative immunofluorescence images (e) and quantification (f) of sciatic nerves labelled with NF200 (red) for visualising nerve fibres and MBP (green) for myelin sheath. Diabetic mice exhibited axonal and myelin damage. Administration of MSC-exosomes reversed the nerve fibre demyelination. n = 5/group. Scale bar, 50 μm. Data are mean ± SEM and were analysed by one-way ANOVA with Tukey’s post hoc test (n = 5/group). †††p < 0.001 db/db vs db/m; **p < 0.01 db/db vs db + exo. db/db, diabetic group treated with saline; db + exo, diabetic group treated with MSC-exosomes; db/m, non-diabetic group

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