Fig. 3 | Diabetologia

Fig. 3

From: Mesenchymal stromal cell-derived exosomes ameliorate peripheral neuropathy in a mouse model of diabetes

Fig. 3

 MSC-exosome treatment increases blood flow perfusion in diabetic mice. (a) Representative laser Doppler images of the plantar skin (footpad; upper mages) and sciatic nerve (lower images) of mice. Flux within tissues was measured and perfusion signals are shown as different colours: blood flow from low to high is shown as blue–green–yellow–orange–red. All of the mice were evaluated under the same experimental conditions. (b) Quantification of blood flow measurement results in (a), expressed in PU/ROI. n = 8/group. (c, d) FITC-dextran perfused vessels from whole mounted sciatic nerves (c), and quantification of FITC-dextran perfused vessel density (d). n = 3/group. Scale bar, 100 μm. (e, f) PGP9.5 staining of IENFs in hind paw plantar skin of all groups (e) and quantification of PGP9.5+ nerve fibre density (f). The epidermis showed a profound loss of PGP9.5+ IENF density in the db/db group. Treatment with MSC-exosomes reversed the diabetes-induced loss of IENFs. n = 8/group. Scale bar, 50 μm. Data are mean ± SEM and were analysed by one-way ANOVA with Tukey’s post hoc test. ††p < 0.01, †††p < 0.001 db/db vs db/m; *p < 0.05 db/db vs db + exo. db/db, diabetic group treated with saline; db + exo, diabetic group treated with MSC-exosomes; db/m, non-diabetic group

Back to article page