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Mapping of a QTL with major effect on reducing leaf rust severity at the adult plant growth stage on chromosome 2BL in wheat landrace Hongmazha

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A major QTL (QLr.cau-2BL) for APR to leaf rust was detected on 2BL; an SSR marker was developed to closely link with QLr.cau-2BL and validated for effectiveness of MAS.

Abstract

The wheat landrace Hongmazha (HMZ) possesses adult plant resistance (APR) to leaf rust. To detect and validate quantitative trait locus (QTL) for the APR, four wheat populations were assessed for leaf rust severity in a total of eight field and greenhouse experiments. The mapping population Aquileja × HMZ (120 recombinant inbred lines, RILs) was genotyped using 90 K SNP markers. A major QTL (QLr.cau-2BL) was detected between the markers IWB3854 and IWB21922 on chromosome 2BL. IWB3854 and IWB21922 were positioned at approximately 531.14 Mb and 616.48 Mb, respectively, on 2BL of IWGSC RefSeq v1.0 physical map. Based on the sequences between 531.14 and 616.48 Mb on 2BL of IWGSC RefSeq v1.0, 415 simple sequence repeat (SSR) markers were developed. These markers and 28 previously published SSR makers were screened; the resulted polymorphic markers were used to genotype the relatively larger population RL6058 × HMZ (371 RILs). QLr.cau-2BL was mapped within a 1.5 cM interval on 2BL map of RL6058 × HMZ, and a marker (Ta2BL_ssr7) was identified to closely link with QLr.cau-2BL. Effectiveness of selection for QLr.cau-2BL based on Ta2BL_ssr7 was validated using two populations (RL6058 × HMZ F2:3 and Jimai22 × HMZ BC4F2:3). In addition, polymorphism at Ta2BL_ssr7 was detected among a panel of 282 commercial wheat cultivars. We believe, therefore, that Ta2BL_ssr7 should be useful for introducing QLr.cau-2BL into commercial wheat cultivars and for accumulating QLr.cau-2BL with other APR QTL.

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Abbreviations

2BL:

The long arm of chromosome 2B

ANOVA:

Analysis of variance

APR:

Adult plant resistance

AUDPC:

The area under the disease progress curve

H 2 :

Broad-sense heritability

HMZ:

Hongmazha, a wheat landrace

IT:

Infection type

LOD:

Logarithm of odds

Lr gene:

Leaf rust resistance gene

MAS:

Marker-assisted selection

PVE:

Phenotypic variation explained

QTL:

Quantitative trait locus / loci

RIL:

Recombinant inbred line

SNP:

Single nucleotide polymorphism

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Acknowledgement

We thank Junzhi Wang, Xinfa Mou, Xinhu Wang, Zonghai Bai, and Yanrun Lin for providing excellent technical assistance during the field work. We thank Dr. Ziyi Du for providing the picture of Figure 1. We acknowledge the enormous help of numerous graduate, undergraduate, and high school students who were associated with the Program for Accumulating Quantitative Resistance to Diseases, Plant Pathology Department, China Agricultural University. This study was supported by the China Postdoctoral Science Foundation (2020M670538), National Key Research and Development Program of China (2016YFD02000 and 2018YFD0200500), and Beijing Academy of Agriculture and Forestry Sciences Sci-Tech Innovation Capacity Building Program (KJCX20200115).

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Author notes

  1. Y. Zhang and Z. Wang contributed equally to this work.

Authors and Affiliations

  1. Department of Plant Pathology, China Agricultural University, Beijing, 100193, People’s Republic of China

    Yibin Zhang, Zhen Wang, Xu Jiang & Zhongjun Zhang

  2. Beijing Engineering and Technique Research Center for Hybrid Wheat, Beijing Academy of Agricultural and Forestry Sciences, Beijing, 100097, People’s Republic of China

    Wei Quan

  3. National Fisheries Technology Extension Center, Beijing, 100125, People’s Republic of China

    Xiang Zhang

  4. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, People’s Republic of China

    Jing Feng

  5. Key Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs, Beijing University of Agriculture, Beijing, 102206, People’s Republic of China

    Junda Ren

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  1. Yibin Zhang
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Contributions

ZZ and WQ conceived the study. YZ and ZW designed and managed the experiments, developed the SSR markers of 2BL, and constructed the genetic maps. YZ, ZW, WQ, JF, JR, ZZ and XJ performed the disease experiments and/or SSR genotyping. XZ statistically analyzed the disease data. ZZ and XZ collected the disease data from greenhouse experiment. ZZ, XZ, QW and JF developed the RILs. YZ and ZW drafted the manuscript. All authors read and approved the final manuscript.

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Correspondence to Wei Quan or Zhongjun Zhang.

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The authors declare that the experiments comply with the current laws of China.

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Communicated by Urmil Bansal.

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Zhang, Y., Wang, Z., Quan, W. et al. Mapping of a QTL with major effect on reducing leaf rust severity at the adult plant growth stage on chromosome 2BL in wheat landrace Hongmazha. Theor Appl Genet 134, 1363–1376 (2021). https://doi.org/10.1007/s00122-021-03776-4

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