A major QTL (QLr.cau-2BL) for APR to leaf rust was detected on 2BL; an SSR marker was developed to closely link with QLr.cau-2BL and validated for effectiveness of MAS.
The wheat landrace Hongmazha (HMZ) possesses adult plant resistance (APR) to leaf rust. To detect and validate quantitative trait locus (QTL) for the APR, four wheat populations were assessed for leaf rust severity in a total of eight field and greenhouse experiments. The mapping population Aquileja × HMZ (120 recombinant inbred lines, RILs) was genotyped using 90 K SNP markers. A major QTL (QLr.cau-2BL) was detected between the markers IWB3854 and IWB21922 on chromosome 2BL. IWB3854 and IWB21922 were positioned at approximately 531.14 Mb and 616.48 Mb, respectively, on 2BL of IWGSC RefSeq v1.0 physical map. Based on the sequences between 531.14 and 616.48 Mb on 2BL of IWGSC RefSeq v1.0, 415 simple sequence repeat (SSR) markers were developed. These markers and 28 previously published SSR makers were screened; the resulted polymorphic markers were used to genotype the relatively larger population RL6058 × HMZ (371 RILs). QLr.cau-2BL was mapped within a 1.5 cM interval on 2BL map of RL6058 × HMZ, and a marker (Ta2BL_ssr7) was identified to closely link with QLr.cau-2BL. Effectiveness of selection for QLr.cau-2BL based on Ta2BL_ssr7 was validated using two populations (RL6058 × HMZ F2:3 and Jimai22 × HMZ BC4F2:3). In addition, polymorphism at Ta2BL_ssr7 was detected among a panel of 282 commercial wheat cultivars. We believe, therefore, that Ta2BL_ssr7 should be useful for introducing QLr.cau-2BL into commercial wheat cultivars and for accumulating QLr.cau-2BL with other APR QTL.
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The long arm of chromosome 2B
Analysis of variance
Adult plant resistance
The area under the disease progress curve
- H 2 :
Hongmazha, a wheat landrace
Logarithm of odds
- Lr gene:
Leaf rust resistance gene
Phenotypic variation explained
Quantitative trait locus / loci
Recombinant inbred line
Single nucleotide polymorphism
Azzimonti G, Marcel TC, Robert O, Paillard C, Lannou C, Goyeau H (2014) Diversity, specificity and impacts on field epidemics of QTL involved in components of quantitative resistance in the wheat leaf rust pathosystem. Mol Breed 34:549–567
Bassam BJ, Caetano-Anolles G, Gresshoff PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem 196:80–83
Brown-Guerdira GL, Singh S, Fritz AK (2003) Performance and mapping of leaf rust resistance to wheat from Triticum timopheevii subsp. ameniacum. Phytopathology 93:784–789
Buerstmayr M, Matiasch L, Mascher F, Vida G, Ittu M, Robert O, Holdgate S, Flath K, Neumayer A, Buerstmayr H (2014) Mapping of quantitative adult plant field resistance to leaf rust and stripe rust in two European winter wheat populations reveals colocation of three QTL conferring resistance to both rust pathogen. Theor Appl Genet 127:2011–2028
Caldwell RM, Schafer JF, Compton LE, Patterson FL (1957) A mature plant type of wheat leaf-rust resistance of composite origin. Phytopathology 47:691–692
Clark JA (1935) Registration of improved wheat varieties VIII. J Am Soc Agron 27(1):71
Dangl JL, Horvath DM, Staskawicz BJ (2013) Pivoting the plant immune system from dissection to deployment. Science 341:746–751
Du ZY, Che MZ, Li GH, Chen J, Quan W, Guo Y, Wang Z, Ren JD, Zhang HY, Zhang ZJ (2015) A QTL with major effect on reducing leaf rust severity on the short arm of chromosome 1A of wheat detected across different genetic backgrounds and diverse environments. Theor Appl Genet 128:1579–1594
Gerard GS, Kobiljskic B, Lohwasserd U, Borner A, Simon MR (2018) Genetic architecture of adult plant resistance to leaf rust in a wheat association mapping panel. Plant Pathol 67:584–594
Herrera-Foessel SA, Singh RP, Huerta-Espino J, Rosewarne GM, Periyannan SK, Viccars J, Calvo-Salazar V, Lan C, Lagudah ES (2012) Lr68: a new gene conferring slow rusting resistance to leaf rust in wheat. Theor Appl Genet 124:1475–1486
Holland JB, Nyquist WE, Cervantes-Martinez C (2003) Estimating and interpreting heritability for plant breeding: an update. Plant Breeding Reviews 22:9–112
Huerta-Espino J, Singh RP, Germán S, McCallum BD, Park RF, Chen WQ, Bhardwaj SC, Goyeau H (2011) Global status of wheat leaf rust caused by Puccinia triticina. Euphytica 179:143–160
International Wheat Genome Sequencing Consortium (IWGSC) (2014) A chromosome-based draft sequence of the hexaploid bread wheat (Triticum aestivum) genome. Science 345:1251788
International Wheat Genome Sequencing Consortium (IWGSC) (2018) Shifting the limits in wheat research and breeding using a fully annotated reference genome. Science 361:7191–7203
Johnson R (1981) Durable resistance: definition of, genetic control, and attainment in plant breeding. Phytopathology 71:567–568
Joukhadar R, Hollaway G, Shi F, Kant S, Forrest K, Wong D, Petkowski J, Pasam R, Tibbits J, Bariana H, Bansal U, Spangenberg G, Daetwyler H, Gendall T, Hayden M (2020) Genome-wide association reveals a complex architecture for rust resistance in 2300 worldwide bread wheat accessions screened under various Australian conditions. Theor Appl Genet 133:2695–2712
Kolmer JA (2015) Collections of Puccinia triticina in different provinces of China are highly related for virulence and molecular genotype. Phytopathology 105:700–706
Kolmer JA, Garvin DF, Jin Y (2011) Expression of a Thatcher wheat adult plant stem rust resistance QTL on chromosome arm 2BL is enhanced by Lr34. Crop Sci 51:526–533
Krattinger SG, Lagudah ES, Spielmeyer W, Singh RP, Huerta-Espino J, McFadden H, Bossolini E, Selter LL, Keller B (2009) A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science 323:1360–1363
Kuraparthy V, Sood S, Chhuneja P, Dhaliwal HS, Kaur S, Bowden RL, Gill BS (2007) A cryptic wheat-Aegilops triuncialis translocation with leaf rust resistance gene Lr58. Crop Sci 47:1995–2003
Lagudah ES (2011) Molecular genetics of race non-specific rust resistance in wheat. Euphytica 179:81–91
Lagudah ES, Krattinger SG, Herrera-Foessel S, Singh RP, Huerta-Espino J, Spielmeyer W, Brown-Guedira G, Selter LL, Keller B (2009) Gene-specific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens. Theor Appl Genet 119:889–898
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Li HH, Ye GY, Wang JK (2007) A modified algorithm for the improvement of composite interval mapping. Genetics 175:361–374
Li ZF, Xia XC, He ZH, Li X, Zhang LJ, Wang HY, Meng QF, Yang WX, Li GQ, Liu DQ (2010) Seedling and slow rusting resistance to leaf rust in Chinese wheat cultivars. Plant Dis 94:45–53
Liu TG, Chen WQ (2012) Race and virulence dynamics of Puccinia triticina in China during 2000–2006. Plant Dis 96:1601–1607
Long DL, Kolmer JA (1989) A North American system of nomenclature for Puccinia recondita f. sp. tritici. Phytopathology 79:525–529
Long DL, Kolmer JA, Leonard KJ, Hughes ME (2002) Physiologic specialization of Puccinia triticina on wheat in the United States in 2000. Plant Dis 86:981–986
Ma Y, Liu T, Liu B, Gao L, Chen W (2020) Population genetic structures of Puccinia triticina in five provinces of China. Eur J Plant Pathol 156:1135–1145
McDonald RA, Linde C (2002) Pathogen population genetics, evolutionary potential, and durable resistance. Annu Rev Phytopathol 40:349–379
McIntosh RA, Dubcovsky J, Rogers JW, Morris C, Xia CX (2017) Catalogue of gene symbols for wheat: 2017 supplement. https ://shige n.nig.ac.jp/wheat /komug i/genes /macge ne/suppl ement 2017.pdf. Accessed from 23 Aug 2020
Moore JW, Herrera-Foessel S, Lan CX, Schnippenkoetter W, Ayliffe M, Huerta-Espino J, Lillemo M, Viccars L, Milne R, Periyannan S, Kong X, Spielmeyer W, Talbot M, Bariana H, Patrick JW, Dodds P, Singh R, Lagudah E (2015) A recently evolved hexose transporter variant confers resistance to multiple pathogens in wheat. Nat Genet 47:1494–1498
Nelson JC, Singh RP, Autrique JE, Sorrells ME (1997) Mapping genes conferring and suppressing leaf rust resistance in wheat. Crop Sci 37:1928–1935
Park RF, McIntosh RA (1994) Adult plant resistances to Puccinia recondita f. sp. tritici in wheat. N Z J Crop Hortic Sci 22:151–158
Peterson RF, Campbell AB, Hannah AE (1948) A diagrammatic scale for estimating rust intensity of leaves and stems of cereals. Can J Res 26:496–500
Qureshi N, Bariana H, Kumran VV, Muruga S, Forrest KL, Hayden MJ, Bansal U (2018) A new leaf rust resistance gene Lr79 mapped in chromosome 3BL from the durum wheat landrace Aus26582. Theor Appl Genet 131:1091–1098
Ren Y, Singh RP, Basnet BR, Lan CX, Huerta-Espino J, Lagudah ES, Ponce-Molina LJ (2017) Identification and mapping of adult plant resistance loci to leaf rust and stripe rust in common wheat cultivar Kundan. Plant Dis 101:456–463
Riaz A, Athiyannan N, Periyannan SK, Afanasenko O, Mitrofanova OP, Platz GJ, Aitken EAB, Snowdon RJ, Lagudah ES, Hickey LT, Voss-Fels KP (2018) Unlocking new alleles for leaf rust resistance in the Vavilov wheat collection. Theor Appl Genet 131:127–144
Röder MS, Korzun V, Wandehake K, Planschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023
Rosewarne GM, Herrera-Foessel SA, Singh RP, Huerta-Espino J, Lan CX, He ZH (2013) Quantitative trait loci of stripe rust resistance in wheat. Theor Appl Genet 126:2427–2449
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8018
Silva GBP, Zanella CM, Martinelli JA, Chaves MS, Hiebert CW, McCallum BD, Boyd LA (2018) Quantitative trait loci conferring leaf rust resistance in hexaploid wheat. Phytopathology 108:1344–1354
Singh RP, Mujeeb-Kazi A, Huerta-Espino J (1998) Lr46: a gene conferring slow-rusting resistance to leaf rust in wheat. Phytopathology 88:890–894
Singh RP, Huerta-Espino J, Rajaram S (2000) Achieving near-immunity to leaf and stripe rusts in wheat by combining slow rusting resistance genes. Acta Phytopathol Entomol Hung 35:133–139
Stam P (1993) Construction of integrated genetic linkage maps by means of a new computer package: JOINMAP. Plant J 3:739–744
Turner MK, Kolmer JA, Pumphrey MO, Bulli P, Chao S, Anderson JA (2017) Association mapping of leaf rust resistance loci in a spring wheat core collection. Theor Appl Genet 130:345–361
Wang S, Basten JC, Zeng ZB (2010) Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, NC. http://statgen.ncsu.edu/qtlcart/WQTLCart.htm. Accessed from 24 Jul 2020
Wang S, Wong D, Forrest K, Allen A, Chao S, Huang BE, Maccaferri M, Salvi S, Milner SG, Cattivelli L, Mastrangelo AM, Whan A, Stephen S, Barker G, Wieseke R, Plieske J, International Wheat Genome Sequencing Consortium, Lillemo M, Mather D, Appels R, Dolferus R, Brown-Guedira G, Korol A, Akhunova AR, Feuillet C, Salse J, Morgante M, Pozniak C, Luo MC, Dvorak J, Morell M, Dubcovsky J, Ganal M, Tuberosa R, Lawley C, Mikoulitch I, Cavanagh C, Edwards KJ, Hayden M, Akhunov E (2014) Characterization of polyploid wheat genomic diversity using a high-density 90000 single nucleotide polymorphism array. Plant Biotechnol J 12:787–796
Xu X, Bai G, Carver BF, Shaner GE, Hunger RM (2005a) Molecular characterization of slow leaf-rusting resistance in wheat. Crop Sci 45:758–765
Xu XY, Bai GH, Carver BF, Shaner GE, Hunger RM (2005b) Mapping of QTL prolonging the latent period of Puccinia triticina infection in wheat. Theor Appl Genet 110:244–251
We thank Junzhi Wang, Xinfa Mou, Xinhu Wang, Zonghai Bai, and Yanrun Lin for providing excellent technical assistance during the field work. We thank Dr. Ziyi Du for providing the picture of Figure 1. We acknowledge the enormous help of numerous graduate, undergraduate, and high school students who were associated with the Program for Accumulating Quantitative Resistance to Diseases, Plant Pathology Department, China Agricultural University. This study was supported by the China Postdoctoral Science Foundation (2020M670538), National Key Research and Development Program of China (2016YFD02000 and 2018YFD0200500), and Beijing Academy of Agriculture and Forestry Sciences Sci-Tech Innovation Capacity Building Program (KJCX20200115).
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Communicated by Urmil Bansal.
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Zhang, Y., Wang, Z., Quan, W. et al. Mapping of a QTL with major effect on reducing leaf rust severity at the adult plant growth stage on chromosome 2BL in wheat landrace Hongmazha. Theor Appl Genet (2021). https://doi.org/10.1007/s00122-021-03776-4