Abstract
Key message
QTL analyses of two bi-parental mapping populations with AC Barrie as a parent revealed numerous FHB-resistance QTL unique to each population and uncovered novel variation near Fhb1.
Abstract
Fusarium head blight (FHB) is a destructive disease of wheat worldwide, leading to severe yield and quality losses. The genetic basis of native FHB resistance was examined in two populations: a recombinant inbred line population from the cross Cutler/AC Barrie and a doubled haploid (DH) population from the cross AC Barrie/Reeder. Numerous QTL were detected among the two mapping populations with many being cross-specific. Photoperiod insensitivity at Ppd-D1 and dwarfing at Rht-B1 and Rht-D1 was associated with increased FHB susceptibility. Anthesis date QTL at or near the Vrn-A1 and Vrn-B1 loci co-located with major FHB-resistance QTL in the AC Barrie/Reeder population. The loci were epistatic for both traits, such that DH lines with both late alleles were considerably later to anthesis and had reduced FHB symptoms (i.e., responsible for the epistatic interaction). Interestingly, AC Barrie contributed FHB resistance near the Fhb1 locus in the Cutler population and susceptibility in the Reeder population. Analyses of the Fhb1 candidate genes PFT and TaHRC confirmed that AC Barrie, Cutler, and Reeder do not carry the Sumai-3 Fhb1 gene. Resistance QTL were also detected at the expected locations of Fhb2 and Fhb5. The native FHB-resistance QTL detected near Fhb1, Fhb2, and Fhb5 do not appear to be as effective as Fhb1, Fhb2, and Fhb5 from Sumai-3. The presence of awns segregated at the B1 awn inhibitor locus in both populations, but was only associated with FHB resistance in the Cutler/AC Barrie population suggesting linkage caused the association rather than pleiotropy.
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Data supporting the current study can be obtained by contacting the corresponding author (curt.mccartney@canada.ca).
Abbreviations
- ANOVA:
-
Analysis of variance
- BLAST:
-
Basic local alignment search tool
- BLUPs:
-
Best linear unbiased predictors
- C/B:
-
Cutler/AC Barrie
- DH:
-
Doubled haploid
- ICIM:
-
Inclusive composite interval mapping
- IM:
-
Interval mapping
- LOD:
-
Logarithm of odds
- META-R:
-
Multi-environment trial analysis with R
- PVE:
-
Phenotypic variation explained
- QTL:
-
Quantitative trait loci
- B/R:
-
AC Barrie/Reeder
- RIL:
-
Recombinant inbred line
- SNP:
-
Single-nucleotide polymorphism
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Acknowledgements
The authors thank technical staff from the participating laboratories for their contributions to this research. The authors wish to acknowledge Dr. Mark Jordan for providing laboratory facilities to carry out FHB candidate gene sequencing work. This project was funded by AAFC Growing Forward II, Western Grains Research Foundation, and as part of CTAG and CTAG2, Genome Prairie projects funded by Genome Canada, Manitoba Agriculture, Saskatchewan Ministry of Agriculture, and Western Grain Research Foundation.
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DT developed the linkage maps, conducted QTL analysis, characterized candidate genes, interpreted the data, and co-wrote the manuscript. AB, BB, GF, AF, JG, MH, DM, RM, BM, DS, MI, CP, and AN designed and conducted the field experiments and generated Fusarium head blight data. CM planned and organized the study, participated in the analysis and interpretation of data, and co-wrote the manuscript. All authors contributed to and approved the final manuscript.
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Supplementary file 2: Figure S1
CLUSTAL alignment of (a) DNA sequences and (b) deduced amino acid sequences of the predicted complete ORF of TaHRC gene amplified from a panel of wheat cultivars including three parents AC Barrie, Cutler, and Reeder using gene specific primer pair TaHRC-GSM-F/TaHRC-GSM-R (Su et al. 2018). Identical residues indicated by asterisks (*) and gaps are identified by dashes. Conserved amino acid substitutions are denoted with colon (:) and semi-conserved substitutions are indicated by a dot (.). Numbers on the right indicate the position number. The red rectangular frames show the locations of the start (ATG (+1)) and stop (TAA) codons, respectively. Fhb1-resistant haplotypes similar to Hap_Ning (Su et al. 2019) carried a large deletion including the start codon (ATG (+1)) and a 22 bp sequence downstream of the original ATG (shown in figure S1a), and part of upstream sequence (not shown in the Figure S1a) (PDF 134 kb)
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Thambugala, D., Brûlé-Babel, A.L., Blackwell, B.A. et al. Genetic analyses of native Fusarium head blight resistance in two spring wheat populations identifies QTL near the B1, Ppd-D1, Rht-1, Vrn-1, Fhb1, Fhb2, and Fhb5 loci. Theor Appl Genet 133, 2775–2796 (2020). https://doi.org/10.1007/s00122-020-03631-y
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DOI: https://doi.org/10.1007/s00122-020-03631-y