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Production of a complete set of wheat–barley group-7 chromosome recombinants with increased grain β-glucan content

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Wheat–barley group-7 recombinant chromosomes were selected using molecular cytogenetics and SNP markers; increased grain β-glucan content was observed in wheat plants with two and four copies of HvCslF6.

Abstract

The soluble dietary fiber (1–3)(1–4) mixed linked β-d-glucan from cereal grains is a valuable component of a healthy diet, which reduces risks of coronary disease and diabetes. Although wheat is an important cereal crop providing a substantial portion of daily calories and protein intake in the human diet, it has a low level of β-glucan. Owing to the plasticity of the polyploid wheat genome, agronomically important traits absent in the wheat primary gene pool can be introgressed from distant relatives. Barley (Hordeum vulgare L.) has a high grain β-glucan content. Earlier, we introgressed this trait into wheat in the form of whole arm compensating Robertsonian translocations (RobT) involving group-7 chromosomes of barley and all three sub-genomes of hexaploid wheat (Triticum aestivum L). In the presented research, we shortened the barley 7HL arms in these RobTs to small pericentromeric segments, using induced wheat–barley homoeologous recombination. The recombinants were selected using SNP markers and molecular cytogenetics. Plants, comprising barley cellulose synthase-like F6 gene (HvCslF6), responsible for β-glucan synthesis, had a higher grain β-glucan content than the wheat control. Three wheat–barley group-7 recombinant chromosomes involving the A, B and D sub-genomes laid the basis for a multiple-copy gene introgression to hexaploid wheat. It is hypothesized that further increases in the β-glucan content in wheat grain can be obtained by increasing the number of HvCslF6 copies through combining several recombinant chromosomes in one line. The wheat lines with four copies of HvCslF6 exceeded the β-glucan content of the lines with two copies.

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Acknowledgements

We thank W. John Raupp for critical editorial review of the manuscript, Duane Wilson for technical assistance, Dr. Gengjun Chen for providing equipment for grain moisture measurement; Dr. Alina Akhunova for real time PCR and spectrophotometry equipment; Drs. Anita Dille and James Stack for providing growth chambers. This is contribution number 19-288-J from the Kansas Agricultural Experiment Station, Kansas State University, Manhattan, KS 66506-5502, U.S.A.

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  1. Tatiana V. Danilova

    Present address: Department of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA

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  1. Department of Plant Pathology, Wheat Genetics Resource Center, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS, 66506-5502, USA

    Tatiana V. Danilova, Jesse Poland & Bernd Friebe

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122_2019_3411_MOESM1_ESM.pdf

Fig. S1 Karyotypes of plants, homozygous for recombinant, translocation chromosomes (pointed by arrows) and their combinations. a. T7AS·7HL-7AL, b. T7BS·7HL-7BL, c. T7DS·7HL-7DL d. T7AS·7ALdel·7HL, e. T7AS·7HL-7AL + RobT7BS·7HL, f. T7AS·7HL-7AL + RobT7DS·7HL. FGISH images: barley chromatin is green, (GAA)n repeat is white, pAs1 repeat is red, chromosomes counterstained with DAPI are blue. Bar = 10 μm

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Danilova, T.V., Poland, J. & Friebe, B. Production of a complete set of wheat–barley group-7 chromosome recombinants with increased grain β-glucan content. Theor Appl Genet 132, 3129–3141 (2019). https://doi.org/10.1007/s00122-019-03411-3

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