Skip to main content
SpringerLink
Log in

Pm62, an adult-plant powdery mildew resistance gene introgressed from Dasypyrum villosum chromosome arm 2VL into wheat

  • Original Article
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Key message

Pm62, a novel adult-plant resistance (APR) gene against powdery mildew, was transferred from D. villosum into common wheat in the form of Robertsonian translocation T2BS.2VL#5.

Abstract

Powdery mildew, which is caused by the fungus Blumeria graminis f. sp. tritici, is a major disease of wheat resulting in substantial yield and quality losses in many wheat production regions of the world. Introgression of resistance from wild species into common wheat has application for controlling this disease. A Triticum durum-Dasypyrum villosum chromosome 2V#5 disomic addition line, N59B-1 (2n = 30), improved resistance to powdery mildew at the adult-plant stage, which was attributable to chromosome 2V#5. To transfer this resistance into bread wheat, a total of 298 BC1F1 plants derived from the crossing between N59B-1 and Chinese Spring were screened by combined genomic in situ hybridization and fluorescent in situ hybridization, 2V-specific marker analysis, and reaction to powdery mildew to confirm that a dominant adult-plant resistance gene, designated as Pm62, was located on chromosome 2VL#5. Subsequently, the 2VL#5 (2D) disomic substitution line (NAU1825) and the homozygous T2BS.2VL#5 Robertsonian translocation line (NAU1823), with normal plant vigor and full fertility, were identified by molecular and cytogenetic analyses of the BC1F2 generation. The effects of the T2BS.2VL#5 recombinant chromosome on agronomic traits were also evaluated in the F2 segregation population. The results suggest that the translocated chromosome may have no distinct effect on plant height, 1000-kernel weight or flowering period, but a slight effect on spike length and seeds per spike. The translocation line NAU1823 has being utilized as a novel germplasm in breeding for powdery mildew resistance, and the effects of the T2BS.2VL#5 recombinant chromosome on yield-related and flour quality characters will be further assessed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Bettgenhaeuser J, Gilbert B, Ayliffe M, Moscou MJ (2014) Non-host resistance to rust pathogens—a continuation of continua. Front Plant Sci 5:664

    Article  Google Scholar 

  • Blanco A, Simeone R (1988) Use of Dasypyrum villosum (L.) Candargy in durum wheat improvement. In: Wittmer G (ed) Proceedings of 3rd international symposium on durum wheat. Foggia, Italy, pp 215–228

  • Chen PD, Qi LL, Zhou B, Zhang SZ, Liu DJ (1995) Development and molecular cytogenetic analysis of wheat-Haynaldia villosa 6VS/6AL translocation lines specifying resistance to powdery mildew. Theor Appl Genet 91:1125–1128

    CAS  PubMed  Google Scholar 

  • Conner RL, Kuzyk AD, Su H (2003) Impact of powdery mildew on the yield of soft white spring wheat cultivars. Can J Plant Sci 83:725–728

    Article  Google Scholar 

  • De Pace C, Vaccino P, Cionini G, Pasquini M, Bizzarri M, Qualset CO (2011) Dasypyrum. In: Kole C (ed) Wild crop relatives: genomic and breeding resources, cereals, vol 1, chapter 4. Springer, Heidelberg, pp 185–292

  • Dieguez MJ, Pergolesi MF, Velasquez SM, Ingala L, Lopez M, Darino M, Paux E, Feuillet C, Sacco F (2014) Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS. Theor Appl Genet 127:1133–1141

    Article  CAS  Google Scholar 

  • Du P, Zhuang LF, Wang YZ, Li Y, Wang Q, Wang DR, Dawa DD, Tan LJ, Shen J, Xu HB, Zhao H, Chu CG, Qi ZJ (2017) Development of oligonucleotides and multiplex probes for quick and accurate identification of wheat and Thinopyrum bessarabicum chromosomes. Genome 60:93–103

    Article  CAS  Google Scholar 

  • Favret EA, Saione HA, Franzone PM (1983) New approaches in breeding for disease resistance. In: Cereal breeding and production symposium. Argentina Special Report 718. Oregon State University

  • Gradzielewska A (2006) The genus Dasypyrum—part 2. Dasypyrum villosum: a wild species used in wheat improvement. Euphytica 152:441–454

    Article  Google Scholar 

  • Hysing SC, Hsam SLK, Singh RP, Huerta-Espino J, Boyd LA, Koebner RMD, Cambron S, Johnson JW, Bland DE, Liljeroth E, Merker A (2007) Agronomic performance and multiple disease resistance in T2BS.2RL wheat-rye translocation lines. Crop Sci 47:254–260

    Article  Google Scholar 

  • Johnson R (1981) Durable resistance: definition of, genetic control, and attainment in plant breeding. Phytopathology 71:567–568

    Article  Google Scholar 

  • Kumar N, Kulwal PL, Balyan HS, Gupta PK (2007) QTL mapping for yield and yield contributing traits in two mapping populations of bread wheat. Mol Breed 19:163–177

    Article  Google Scholar 

  • Law CN, Sutka J, Worland AJ (1978) A genetic study of day-length response in wheat. Heredity 41:185–191

    Article  Google Scholar 

  • Li K, Hegarty J, Zhang CZ, Wan AM, Wu JJ, Guedira GB, Chen XM, Amatriain MM, Fu DL, Dubcovsky J (2016) Fine mapping of barley locus Rps6 conferring resistance to wheat stripe rust. Theor Appl Genet 129:845–859

    Article  CAS  Google Scholar 

  • Liu DJ, Chen PD, Pei GZ, Wang YN, Qiu BX, Wang SL (1988) Transfer of Haynaldia villosa chromosomes into Triticum aestivum. In: Miller TE, Koebner RMD (eds) Proceedings of the 7th international wheat genetics symposium. Institute of Plant Sciences Research, Cambridge, England, pp 355–361

  • Liu C, Qi LL, Liu WX, Zhao WC, Wilson J, Friebe B, Gill BS (2011) Development of a set of compensating Triticum aestivum–Dasypyrum villosum Robertsonian translocation lines. Genome 54:836–844

    Article  CAS  Google Scholar 

  • Liu N, Liu ZL, Gong G, Zhang M, Wang X, Zhou Y, Qi XB, Chen HB, Yang JZ, Lio PG, Yang CP (2015) Virulence structure of Blumeria graminis f. sp. tritici and its genetic diversity by ISSR and SRAP profiling analyses. PLoS ONE. https://doi.org/10.1371/0130881

    Article  PubMed  PubMed Central  Google Scholar 

  • Liu WX, Koo DH, Xia Q, Li CX, Bai F, Song YL, Friebe B, Gill BS (2017) Homoeologous recombination-based transfer and molecular cytogenetic mapping of powdery mildewresistant gene Pm57 from Aegilops searsii into wheat. Theor Appl Genet 130:841–848

    Article  CAS  Google Scholar 

  • Lukaszewski AJ (1988) A comparison of several approaches in the development of disomic alien addition lines of wheat. In: Miller TE, Koebner RMD (eds) Proceedings of the 7th international wheat genetics symposium, vol 1. Institute of Plant Sciences Research, Cambridge, UK, pp 363–367

  • Lukaszewski AJ, Cowger C (2017) Re-engineering of the Pm21 transfer from Haynaldia villosa to bread wheat by induced homoeologous recombination. Crop Sci 57:2590–2594

    Article  CAS  Google Scholar 

  • McIntosh RA, Welllings CR, Park RF (1995) Wheat rusts: an atlas of resistance genes. Kluwer, Dordrecht

    Book  Google Scholar 

  • McIntosh RA, Yamazaki Y, Dubcovsky J, Morris C, Appels R, Xia XC (2013) Catalogue of gene symbols for wheat. In: Ogihara Y (ed) Proceeding of the 12th international wheat genetics symposium, Yokohama, Japan, 8–13 September 2013, pp 8–13

  • McIntosh RA, Dubcovsky J, Rogers WJ, Morris C, Appels R, Xia XC (2017) Catalogue of gene symbols for wheat: 2017 Supplement. Annu Wheat Newsl 53:1–20

    Google Scholar 

  • Messmer MM, Seyfarth R, Keller M, Schachermayr G, Winzeler M, Zanetti S, Feuillet C, Keller B (2000) Genetic analysis of durable leaf rust resistance in winter wheat. Theor Appl Genet 100:419–431

    Article  CAS  Google Scholar 

  • Muramatsu M (2009) A presumed genetic system determining the number of spikelets per rachis node in the tribe Triticeae. Breed Sci 59:617–620

    Article  Google Scholar 

  • Qi LL, Friebe B, Zhang P, Gill BS (2007) Homoeologous recombination, chromosome engineering and crop improvement. Chromosome Res 15:3–19

    Article  CAS  Google Scholar 

  • Qi ZJ, Du P, Qian BL, Zhuang LF, Chen HF, Chen TT, Shen J, Guo J, Feng YG, Pei ZY (2010) Characterization of a wheat-Thinopyrum bessarabicum (T2JS-2BS.2BL) translocation line. Theor Appl Genet 121:589–597

    Article  CAS  Google Scholar 

  • Qi LL, Pumphrey MO, Friebe B, Zhang P, Qian C, Bowden RL, Rouse MN, Jin Y, Gill BS (2011) A novel Robertsonian translocation event leads to transfer of a stem rust resistance gene (Sr52) effective against race Ug99 from Dasypyrum villosum into bread wheat. Theor Appl Genet 123:159–167

    Article  CAS  Google Scholar 

  • Sawhney RN, Nayar SK, Sharma JB, Bedi R (1989) Mechanism of durable resistance: a new approach. Theor Appl Genet 78:229–232

    Article  CAS  Google Scholar 

  • Sears ER (1953) Addition of the genome of Haynaldia villosa to Triticum aestivum. Am J Bot 40:168–174

    Article  Google Scholar 

  • Shaner G (1973) Evaluation of slow-mildewing resistance of Knox wheat in the field. Phytopathology 63:867–872

    Article  Google Scholar 

  • Sheng BQ, Duan XY (1991) Modification on the evaluation methods of 0–9 level of powdery mildew infection on wheat. Biotech J Agric Sci 9:37–39

    Google Scholar 

  • Turner A, Beales J, Faure S, Dunford RP, Laurie DA (2005) The pseudo-response regulator Ppd-H1 provides adaptation to photoperiod in barley. Science 310:1031–1034

    Article  CAS  Google Scholar 

  • Wang ZL, Li LH, He ZH, Duan XY, Zhou YL, Chen XM, Lillemo M, Singh RP, Wanh H, Xia XC (2005) Seedling and adult plant resistance to powdery mildew in Chinese bread wheat cultivars and lines. Plant Dis 89:457–463

    Article  CAS  Google Scholar 

  • Wiersma AT, Pulman JA, Brown LK, Cowger C, Olson EL (2017) Identification of Pm58 from Aegilops tauschii. Theor Appl Genet 130:1123–1133

    Article  CAS  Google Scholar 

  • Xing LP, Hu P, LiuvJQ Witek K, Zhou S, Gao L, Huang ZP, Zhang RQ, Wang XE, Chen PD, Wang HY, Jones J, Karafiátová M, Vrána J, Bartos J, Dolezel J, Tian YC, Wu YF, Cao AZ (2018) Pm21 from Haynaldia villosa encodes a CC-NBS-LRR protein conferring powdery mildew resistance in wheat. Mol Plant. https://doi.org/10.1016/j.molp

    Article  PubMed  Google Scholar 

  • Zhang RQ, Zhang MY, Wang XE, Chen PD (2014) Introduction of chromosome segment carrying the seed storage protein genes from chromosome 1V of Dasypyrum villosum showed positive effect on bread-making quality of common wheat. Theor Appl Genet 127:523–533

    Article  CAS  Google Scholar 

  • Zhang RQ, Hou F, Feng YG, Zhang W, Zhang MY, Chen PD (2015) Characterization of a Triticum aestivum–Dasypyrum villosum T2VS·2DL translocation line expressing a longer spike and more kernels traits. Theor Appl Genet 128:2415–2425

    Article  CAS  Google Scholar 

  • Zhang RQ, Sun BX, Chen J, Cao AZ, Xing LP, Feng YG, Lan CX, Chen PD (2016) Pm55, a developmental-stage and tissue specific powdery mildew resistance gene introgressed from Dasypyrum villosum into common wheat. Theor Appl Genet 129:1975–1984

    Article  CAS  Google Scholar 

  • Zhang XD, Wei X, Xiao J, Yuan CX, Wu YF, Cao AZ, Xing LP, Chen PD, Zhang SZ, Wang HY (2017) Whole genome development of intron targeting (IT) markers specific for Dasypyrum villosum chromosomes based on next-generation sequencing technology. Mol Breed 37:115

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank Dr. Robert McIntosh, University of Sydney, for reviewing the manuscript. This work was supported by the Important National Science and Technology Specific Projects of Transgenic Research (2018ZX0800963B); Fundamental Research Funds for the Central Universities (KYZ201809); The National Natural Science Foundation of China (31671685, 31771779, 31871619); and the National Center of Teaching and Experiment for the Plant Science Project of Innovation and Training for Undergraduate Students (ZKF201702).

Author information

Authors and Affiliations

  1. College of Agronomy/JCIC-MCP/National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Ruiqi Zhang, Yali Fan, Lingna Kong, Zuojun Wang, Liping Xing, Aizhong Cao & Yigao Feng

  2. Institute of Germplasm Resources and Biotechnology/Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, People’s Republic of China

    Jizhong Wu

Authors
  1. Ruiqi Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yali Fan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lingna Kong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zuojun Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jizhong Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Liping Xing
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Aizhong Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Yigao Feng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ruiqi Zhang.

Ethics declarations

Conflict of interest

The authors declared that they have no conflict of interest.

Additional information

Communicated by Beat Keller.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, R., Fan, Y., Kong, L. et al. Pm62, an adult-plant powdery mildew resistance gene introgressed from Dasypyrum villosum chromosome arm 2VL into wheat. Theor Appl Genet 131, 2613–2620 (2018). https://doi.org/10.1007/s00122-018-3176-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-018-3176-5

Search

Navigation