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The gene conferring susceptibility to spot blotch caused by Cochliobolus sativus is located at the Mla locus in barley cultivar Bowman

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We identified, fine mapped, and physically anchored a dominant spot blotch susceptibility gene Scs6 to a 125 kb genomic region containing the Mla locus on barley chromosome 1H.

Abstract

Spot blotch caused by Cochliobolus sativus is an important disease of barley, but the molecular mechanisms underlying resistance and susceptibility to the disease are not well understood. In this study, we identified and mapped a gene conferring susceptibility to spot blotch caused by the pathotype 2 isolate (ND90Pr) of C. sativus in barley cultivar Bowman. Genetic analysis of F1 and F2 progeny as well as F3 families from a cross between Bowman and ND 5883 indicated that a single dominant gene (designated as Scs6) conferred spot blotch susceptibility in Bowman. Using a doubled haploid (DH) population derived from a cross between Calicuchima-sib (resistant) and Bowman-BC (susceptible), we confirmed that Scs6, contributed by Bowman-BC, was localized at the same locus as the previously identified spot blotch resistance allele Rcs6, which was contributed by Calicuchima-sib and mapped on the short arm of chromosome 1H. Using a genome-wide putative linear gene index of barley (Genome Zipper), 13 cleaved amplified polymorphism markers were developed from 11 flcDNA and two EST sequences and mapped to the Scs6/Rcs6 region on a linkage map constructed with the DH population. Further fine mapping with markers developed from barley genome sequences and F2 recombinants derived from Bowman × ND 5883 and Bowman × ND B112 crosses delimited Scs6 in a 125 kb genomic interval harboring the Mla locus on the reference genome of barley cv. Morex. This study provides a foundational step for further cloning of Scs6 using a map-based approach.

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Acknowledgements

The authors thank Joseph Mullins, Zhongxing Sun, Qiang Li, and Yue Zhou for assistance in greenhouse phenotyping experiments. This research was funded by the Triticeae-CAP project (2011-68002-30029) of the US Department of Agriculture National Institute of Food and Agriculture.

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Authors and Affiliations

  1. Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108-6050, USA

    Yueqiang Leng, Mingxia Zhao, Rui Wang, Robert S. Brueggeman & Shaobin Zhong

  2. Department of Plant Pathology, University of Minnesota, St. Paul, MN, 55108, USA

    Brian J. Steffenson

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  1. Yueqiang Leng
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  2. Mingxia Zhao
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  3. Rui Wang
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  4. Brian J. Steffenson
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  5. Robert S. Brueggeman
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  6. Shaobin Zhong
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Correspondence to Shaobin Zhong.

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Communicated by Xiaoquan Qi.

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Leng, Y., Zhao, M., Wang, R. et al. The gene conferring susceptibility to spot blotch caused by Cochliobolus sativus is located at the Mla locus in barley cultivar Bowman. Theor Appl Genet 131, 1531–1539 (2018). https://doi.org/10.1007/s00122-018-3095-5

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