Abstract
Key message
Shuhui498 (R498) is an elite parent of heavy panicle hybrid rice by pyramiding the rare gn1a and null gs3 alleles. This finding reveals the genetic basis and great potential application in future breeding of R498.
Abstract
The heavy panicle trait, defined as 5 g or more of grain weight per panicle, is one of the target traits in super-high-yield rice breeding programs. The use of heavy panicle-type hybrid rice has been shown to be a successful strategy for super-high-yield breeding programs, particularly under the environmental conditions of high humidity and deficient solar radiation in southwestern China. However, the genetic components of the heavy panicle trait in hybrid rice remain elusive. Here, we report that the combination of loss-of-function mutations in Grain number 1a (Gn1a) and Grain Size 3 (GS3) is responsible for the heavy panicle phenotype of the elite hybrid rice restorer line Shuhui498 (R498). The null gn1a allele is the determinant factor for heavy panicles through increased grain number, while gs3 is associated with grain size and weight. R498 pyramided the two major null alleles, resulting in heavy panicles with a high grain number and large grains. Clustering analysis revealed that the null gn1aR498 allele is a rare haplotype which has been innovatively utilized in R498, underscoring the great potential of R498 for breeding purposes. Our research thus sheds light on the distinct genetic compositions of heavy panicle-type rice and may potentially facilitate super-high-yield rice breeding.
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Acknowledgements
We thank Prof. Dengcai Liu, Prof. Wenming Wang, and Prof. Xuewei Chen of SCAU (Sichuan Agricultural University) for editing and providing comments regarding this manuscript. This research was supported by Grant NSFC 91735304 and 31571441 from the National Science Foundation of China, Breeding New Cultivar of Super Green Rice (2014AA10A604), State Key Laboratory of Hybrid Rice (2016KF09) and Key Research Project of Sichuan Provincial Education Department (14ZA0013).
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Communicated by Takuji Sasaki.
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Wang, S., Ma, B., Gao, Q. et al. Dissecting the genetic basis of heavy panicle hybrid rice uncovered Gn1a and GS3 as key genes. Theor Appl Genet 131, 1391–1403 (2018). https://doi.org/10.1007/s00122-018-3085-7
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DOI: https://doi.org/10.1007/s00122-018-3085-7