Skip to main content
SpringerLink
Log in

Characterization of Pm59, a novel powdery mildew resistance gene in Afghanistan wheat landrace PI 181356

  • Original Article
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Key message

A new powdery mildew resistance gene, designated Pm59, was identified in Afghanistan wheat landrace PI 181356, and mapped in the terminal region of the long arm of chromosome 7A.

Abstract

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is an important foliar disease of wheat worldwide. In the Great Plains of the USA, Bgt isolates virulent to widely used powdery mildew resistance genes, such as Pm3a, were previously identified. The objectives of this study were to characterize the powdery mildew resistance gene in Afghanistan landrace PI 181356, which exhibited high resistance to Bgt isolates collected in southern Great Plains, and identify molecular markers for marker-assisted selection. An F2 population and F2:3 lines derived from a cross between PI 181356 and OK1059060-126135-3 were used in this study. Genetic analysis indicated that PI 181356 carries a single dominant gene, designated Pm59, in the terminal region of the long arm of chromosome 7A. Pm59 was mapped to an interval between sequence tag site (STS) markers Xmag1759 and Xmag1714 with genetic distances of 0.4 cM distal to Xmag1759 and 5.7 cM proximal to Xmag1714. Physical mapping suggested that Pm59 is in the distal bin 7AL 0.99–1.00. Pm59 is a novel powdery mildew resistance gene, and confers resistance to Bgt isolates collected from the Great Plains and the state of Montana. Therefore, Pm59 can be used to breed powdery mildew-resistant cultivars in these regions. Xmag1759 is ideal for marker-assisted selection of Pm59 in wheat breeding.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

Abbreviations

Bgt :

Blumeria graminis f. sp. tritici

cM:

Centimorgan

Pm gene:

Powdery mildew resistance gene

STS:

Sequence tag site

SSR:

Simple sequence repeat

References

  • Bennett FGA (1984) Resistance to powdery mildew in wheat: a review of its use in agriculture and breeding programmes. Plant Pathol 33:279–300. https://doi.org/10.1111/j.1365-3059.1984.tb01324.x

    Article  Google Scholar 

  • Cenci A, D’ovidio R, Tanzarella OA et al (1999) Identification of molecular markers linked to Pm13, an Aegilops longissima gene conferring resistance to powdery mildew in wheat. Theor Appl Genet 98:448–454

    Article  CAS  Google Scholar 

  • Chen XM, Luo YH, Xia XC et al (2005) Chromosomal location of powdery mildew resistance gene Pm16 in wheat using SSR marker analysis. Plant Breed 124:225–228

    Article  CAS  Google Scholar 

  • Chhuneja P, Kumar K, Stirnweis D et al (2012) Identification and mapping of two powdery mildew resistance genes in Triticum boeoticum L. Theor Appl Genet 124:1051–1058

    Article  CAS  PubMed  Google Scholar 

  • Cowger C, Parks R, Marshall D (2009) Appearance of powdery mildew of wheat caused by Blumeria graminis f. sp. tritici on Pm17-bearing cultivars in North Carolina. Plant Dis 93:1219

    Article  Google Scholar 

  • Cowger C, Mehra LK, Arellano C et al (2017) Virulence differences in Blumeria graminis f. sp. tritici from the central and eastern United States. Phytopathology (accepted)

  • Dubcovsky J, Galvez AF, Dvořák J (1994) Comparison of the genetic organization of the early salt-stress-response gene system in salt-tolerant Lophopyrum elongatum and salt-sensitive wheat. Theor Appl Genet 87:957–964

    Article  CAS  PubMed  Google Scholar 

  • FAOSTAT D (2013) Food and agriculture organization of the United Nations. Statistical database

  • Flor HH (1955) Host-parasite interactions in flax rust-its genetics and other implications. Phytopathology 45:680–685

    Google Scholar 

  • Foley JA, Ramankutty N, Brauman KA et al (2013) Solutions for a cultivated planet. Nat Lond 501:S35–S40

    Google Scholar 

  • Friebe B, Heun M, Tuleen N et al (1994) Cytogenetically monitored transfer of powdery mildew resistance from rye into wheat. Crop Sci 34:621–625

    Article  Google Scholar 

  • Fu B, Chen Y, Li N et al (2013) pmX: a recessive powdery mildew resistance gene at the Pm4 locus identified in wheat landrace Xiaohongpi. Theor Appl Genet 126:913–921

    Article  CAS  PubMed  Google Scholar 

  • Hsam SLK, Huang XQ, Ernst F et al (1998) Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em Thell.). 5. Alleles at the Pm1 locus. Theor Appl Genet 96:1129–1134

    Article  CAS  Google Scholar 

  • Hsam SLK, Huang XQ, Zeller FJ (2001) Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em Thell.) 6. Alleles at the Pm5 locus. Theor Appl Genet 102:127–133

    Article  CAS  Google Scholar 

  • Huang XQ, Hsam SLK, Zeller FJ et al (2000a) Molecular mapping of the wheat powdery mildew resistance gene Pm24 and marker validation for molecular breeding. Theor Appl Genet 101:407–414

    Article  CAS  Google Scholar 

  • Huang XQ, Hsam SLK, Zeller FJ (2000b) Chromosomal location of powdery mildew resistance genes in Chinese wheat (Triticum aestivum L. em. Thell.) landraces Xiaobaidong and Fuzhuang 30. J Genet Amp Breed 54:311–317

    CAS  Google Scholar 

  • Huang X, Wang L, Xu M, Röder M (2003) Microsatellite mapping of the powdery mildew resistance gene Pm5e in common wheat (Triticum aestivum L.). Theor Appl Genet 106:858–865

    Article  CAS  PubMed  Google Scholar 

  • Ji X, Xie C, Ni Z et al (2008) Identification and genetic mapping of a powdery mildew resistance gene in wild emmer (Triticum dicoccoides) accession IW72 from Israel. Euphytica 159:385–390

    Article  CAS  Google Scholar 

  • Johnson JW, Baenziger PS, Yamazaki WT, Smith RT (1979) Effects of powdery mildew on yield and quality of isogenic lines of “Chancellor” wheat. Crop Sci 19:349–352

    Article  Google Scholar 

  • Kosambi DD (1943) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Article  Google Scholar 

  • Lander ES, Green P, Abrahamson J et al (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  CAS  PubMed  Google Scholar 

  • Li G, Xu X, Bai G et al (2016) Identification of novel powdery mildew resistance sources in wheat. Crop Sci 56:1817–1830

    Article  CAS  Google Scholar 

  • Li G, Xu X, Carver, BF et al (2018) Novel wheat powdery mildew resistance loci identified via genome-wide association mapping, and development of associated KASP markers. Sci Rep (Submitted)

  • Ma ZQ, Sorrells ME, Tanksley SD (1994) RFLP markers linked to powdery mildew resistance genes Pm1, Pm2, Pm3, and Pm4 in wheat. Genome 37:871–875

    Article  CAS  PubMed  Google Scholar 

  • Maxwell JJ, Lyerly JH, Cowger C et al (2009) MlAG12: a Triticum timopheevii-derived powdery mildew resistance gene in common wheat on chromosome 7AL. Theor Appl Genet 119:1489–1495

    Article  CAS  PubMed  Google Scholar 

  • McIntosh RA, Yamazaki Y, Dubcovsky J, et al (2013) Catalogue of gene symbols for wheat. In: Ogihara Y (ed) Proceeding of the 12th international wheat genetics symposium, Yokohama, Japan, 8–13 Sept 2013, pp 8–13

  • McIntosh RA, Dubcovsky J, Rogers WJ et al (2017) Catalogue of gene symbols for wheat. 2017. Supplement. Annu Wheat Newsl 53:1–20

    Google Scholar 

  • Miranda LM, Perugini L, Srnić G et al (2007) Genetic mapping of a Triticum monococcum-derived powdery mildew resistance gene in common wheat. Crop Sci 47:2323–2329

    Article  CAS  Google Scholar 

  • Morgounov A, Tufan HA, Sharma R et al (2012) Global incidence of wheat rusts and powdery mildew during 1969–2010 and durability of resistance of winter wheat variety Bezostaya 1. Eur J Plant Pathol Dordr 132:323–340. https://doi.org/10.1007/s10658-011-9879-y

    Article  Google Scholar 

  • Nematollahi G, Mohler V, Wenzel G et al (2008) Microsatellite mapping of powdery mildew resistance allele Pm5d from common wheat line IGV1-455. Euphytica 159:307–313

    Article  CAS  Google Scholar 

  • Neu C, Stein N, Keller B (2002) Genetic mapping of the Lr20-Pm1 resistance locus reveals suppressed recombination on chromosome arm 7AL in hexaploid wheat. Genome 45:737–744

    Article  CAS  PubMed  Google Scholar 

  • Ouyang S, Zhang D, Han J et al (2014) Fine physical and genetic mapping of powdery mildew resistance gene MlIW172 originating from wild emmer (Triticum dicoccoides). PLoS One 9:e100160

    Article  PubMed  PubMed Central  Google Scholar 

  • Parks R, Carbone I, Murphy JP et al (2008) Virulence structure of the eastern US wheat powdery mildew population. Plant Dis 92:1074–1082

    Article  Google Scholar 

  • Perugini LD, Murphy JP, Marshall D, Brown-Guedira G (2008) Pm37, a new broadly effective powdery mildew resistance gene from Triticum timopheevii. Theor Appl Genet 116:417–425

    Article  CAS  PubMed  Google Scholar 

  • Pugsley AT, Carter MV (1953) The resistance of twelve varieties of Triticum vulgare to Erysiphe graminis tritici. Aust J Biol Sci 6:335–346

    Article  CAS  PubMed  Google Scholar 

  • Qi L, Cao M, Chen P et al (1996) Identification, mapping, and application of polymorphic DNA associated with resistance gene Pm21 of wheat. Genome 39:191–197

    Article  CAS  PubMed  Google Scholar 

  • Schneider DM, Heun M, Fischbeck G (1991) Inheritance of the powdery mildew resistance gene Pm9 in relation to Pm1 and Pm2 of wheat. Plant Breed 107:161–164. https://doi.org/10.1111/j.1439-0523.1991.tb00545.x

    Article  Google Scholar 

  • Sears ER, Briggle LW (1969) Mapping the gene Pm1 for resistance to Erysiphe graminis f. sp. tritici on chromosome 7A of wheat. Crop Sci 9:96–97

    Article  Google Scholar 

  • Singrün C (2002) Untersuchungen zur Lokalisierung und Kartierung von Genen für Resistenz gegen Mehltau und Braunrost in Saatweizen (Triticum aestivum L.) und Dinkel (Triticum spelta L.). Dissertation. Technische Universität München

  • Singrün CH, Hsam SLK, Zeller FJ et al (2004) Localization of a novel recessive powdery mildew resistance gene from common wheat line RD30 in the terminal region of chromosome 7AL. Theor Appl Genet 109:210–214

    Article  PubMed  Google Scholar 

  • Srnić G, Murphy JP, Lyerly JH et al (2005) Inheritance and chromosomal assignment of powdery mildew resistance genes in two winter wheat germplasm lines. Crop Sci 45:1578–1586

    Article  Google Scholar 

  • Tilman D, Cassman KG, Matson PA et al (2002) Agricultural sustainability and intensive production practices. Nat Lond 418:671–677. https://doi.org/10.1038/nature01014

    Article  CAS  Google Scholar 

  • Wang ZL, Li LH, He ZH et al (2005) Seedling and adult plant resistance to powdery mildew in Chinese bread wheat cultivars and lines. Plant Dis 89:457–463

    Article  CAS  Google Scholar 

  • Wang Z, Li H, Zhang D et al (2015) Genetic and physical mapping of powdery mildew resistance gene MlHLT in Chinese wheat landrace Hulutou. Theor Appl Genet 128:365–373

    Article  CAS  PubMed  Google Scholar 

  • Worthington M, Lyerly J, Petersen S et al (2014) MlUM15: an Aegilops neglecta-derived powdery mildew resistance gene in common wheat. Crop Sci 54:1397–1406

    Article  CAS  Google Scholar 

  • Xiao M, Song F, Jiao J et al (2013) Identification of the gene Pm47 on chromosome 7BS conferring resistance to powdery mildew in the Chinese wheat landrace Hongyanglazi. Theor Appl Genet 126:1397–1403

    Article  CAS  PubMed  Google Scholar 

  • Xu H, Yi Y, Ma P et al (2015) Molecular tagging of a new broad-spectrum powdery mildew resistance allele Pm2c in Chinese wheat landrace Niaomai. Theor Appl Genet 128:2077–2084

    Article  CAS  PubMed  Google Scholar 

  • Xue F, Wang C, Li C et al (2012) Molecular mapping of a powdery mildew resistance gene in common wheat landrace Baihulu and its allelism with Pm24. Theor Appl Genet 125:1425–1432

    Article  CAS  PubMed  Google Scholar 

  • Yahiaoui N, Srichumpa P, Dudler R, Keller B (2004) Genome analysis at different ploidy levels allows cloning of the powdery mildew resistance gene Pm3b from hexaploid wheat. Plant J 37:528–538

    Article  CAS  PubMed  Google Scholar 

  • Yao G, Zhang J, Yang L et al (2007) Genetic mapping of two powdery mildew resistance genes in einkorn (Triticum monococcum L.) accessions. Theor Appl Genet 114:351–358

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We thank Dr. Robert McIntosh of Sydney University for reviewing this paper. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer.

Author information

Author notes

  1. Chengcheng Tan and Genqiao Li contributed equally to this work.

Authors and Affiliations

  1. Wheat, Peanut, and Other Field Crops Research Unit, USDA-ARS, Stillwater, OK, 74075, USA

    Chengcheng Tan, Genqiao Li & Xiangyang Xu

  2. Plant and Soil Science Department, Oklahoma State University, Stillwater, OK, 74078, USA

    Genqiao Li & Brett F. Carver

  3. Plant Science Research Unit, USDA-ARS, Raleigh, NC, 27695, USA

    Christina Cowger

Authors
  1. Chengcheng Tan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Genqiao Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Christina Cowger
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Brett F. Carver
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xiangyang Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xiangyang Xu.

Ethics declarations

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

Additional information

Communicated by Evans Lagudah.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tan, C., Li, G., Cowger, C. et al. Characterization of Pm59, a novel powdery mildew resistance gene in Afghanistan wheat landrace PI 181356. Theor Appl Genet 131, 1145–1152 (2018). https://doi.org/10.1007/s00122-018-3067-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-018-3067-9

Search

Navigation